MBD8_ARATH
ID MBD8_ARATH Reviewed; 524 AA.
AC Q9LME6; Q8RXV1;
DT 08-MAR-2011, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 113.
DE RecName: Full=Methyl-CpG-binding domain-containing protein 8;
DE Short=AtMBD8;
DE Short=MBD08;
DE AltName: Full=Methyl-CpG-binding protein MBD8;
GN Name=MBD8; OrderedLocusNames=At1g22310; ORFNames=T16E15.7;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP ALTERNATIVE SPLICING, TISSUE SPECIFICITY, GENE FAMILY, AND NOMENCLATURE.
RX PubMed=12954765; DOI=10.1093/nar/gkg735;
RA Berg A., Meza T.J., Mahic M., Thorstensen T., Kristiansen K., Aalen R.B.;
RT "Ten members of the Arabidopsis gene family encoding methyl-CpG-binding
RT domain proteins are transcriptionally active and at least one, AtMBD11, is
RT crucial for normal development.";
RL Nucleic Acids Res. 31:5291-5304(2003).
RN [6]
RP SUBCELLULAR LOCATION.
RX PubMed=14605234; DOI=10.1104/pp.103.026708;
RA Ito M., Koike A., Koizumi N., Sano H.;
RT "Methylated DNA-binding proteins from Arabidopsis.";
RL Plant Physiol. 133:1747-1754(2003).
RN [7]
RP GENE FAMILY.
RX PubMed=15888682; DOI=10.1104/pp.105.060566;
RA Springer N.M., Kaeppler S.M.;
RT "Evolutionary divergence of monocot and dicot methyl-CpG-binding domain
RT proteins.";
RL Plant Physiol. 138:92-104(2005).
RN [8]
RP REVIEW.
RX PubMed=17208509; DOI=10.1016/j.tplants.2006.12.004;
RA Zemach A., Grafi G.;
RT "Methyl-CpG-binding domain proteins in plants: interpreters of DNA
RT methylation.";
RL Trends Plant Sci. 12:80-85(2007).
RN [9]
RP FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, AND DEVELOPMENTAL
RP STAGE.
RC STRAIN=cv. C24, and cv. Columbia;
RX PubMed=19374717; DOI=10.1111/j.1399-3054.2009.01218.x;
RA Stangeland B., Rosenhave E.M., Winge P., Berg A., Amundsen S.S.,
RA Karabeg M., Mandal A., Bones A.M., Grini P.E., Aalen R.B.;
RT "AtMBD8 is involved in control of flowering time in the C24 ecotype of
RT Arabidopsis thaliana.";
RL Physiol. Plantarum 136:110-126(2009).
CC -!- FUNCTION: Probable transcriptional regulator (By similarity). May
CC regulates developmental traits such as flowering time. {ECO:0000250,
CC ECO:0000269|PubMed:19374717}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:14605234}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1; Synonyms=AtMBD8a;
CC IsoId=Q9LME6-1; Sequence=Displayed;
CC Name=2; Synonyms=AtMBD8b;
CC IsoId=Q9LME6-2; Sequence=VSP_040660;
CC -!- TISSUE SPECIFICITY: Expressed in shoot meristems, roots (vasculature
CC and tips), hypocotyls (vasculature), cotyledons (vasculature and
CC hydathodes), young leaves, buds, flowers and stems. Detected in
CC stomata. {ECO:0000269|PubMed:12954765, ECO:0000269|PubMed:19374717}.
CC -!- DEVELOPMENTAL STAGE: Before pollination, expressed in stamens and
CC carpels. After pollination, observed in the veins of petals, sepals,
CC and filaments. Present in seeds at the embryo globular stage, both in
CC the embryo and the endosperm. Later, at the embryo heart stage, not
CC detected in the embryo, but strongly expressed in the maternal chalazal
CC tissue and present in the endosperm. {ECO:0000269|PubMed:19374717}.
CC -!- DOMAIN: The methyl-CpG-binding domain (MBD) functions both in binding
CC to methylated DNA and in protein interactions. {ECO:0000250}.
CC -!- DISRUPTION PHENOTYPE: In strain cv. C24, delayed flowering time during
CC both long and short days associated with a down-regulation of the major
CC promoters of flowering FT and SOC1. In strain cv. Columbia, no delayed
CC phenotype. {ECO:0000269|PubMed:19374717}.
CC -!- MISCELLANEOUS: In strain cv. 24, promotes the flowering time
CC transition, probably via the transcription regulation of FT.
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DR EMBL; AC068562; AAF87260.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE30228.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE30229.1; -; Genomic_DNA.
DR EMBL; AK118852; BAC43440.1; -; mRNA.
DR EMBL; AY080662; AAL86338.1; -; mRNA.
DR EMBL; AY133726; AAM91660.1; -; mRNA.
DR PIR; A86356; A86356.
DR RefSeq; NP_173650.3; NM_102082.4. [Q9LME6-1]
DR RefSeq; NP_850949.1; NM_180618.2. [Q9LME6-2]
DR AlphaFoldDB; Q9LME6; -.
DR BioGRID; 24077; 3.
DR IntAct; Q9LME6; 3.
DR STRING; 3702.AT1G22310.2; -.
DR iPTMnet; Q9LME6; -.
DR PaxDb; Q9LME6; -.
DR PRIDE; Q9LME6; -.
DR ProteomicsDB; 238691; -. [Q9LME6-1]
DR EnsemblPlants; AT1G22310.1; AT1G22310.1; AT1G22310. [Q9LME6-2]
DR EnsemblPlants; AT1G22310.2; AT1G22310.2; AT1G22310. [Q9LME6-1]
DR GeneID; 838838; -.
DR Gramene; AT1G22310.1; AT1G22310.1; AT1G22310. [Q9LME6-2]
DR Gramene; AT1G22310.2; AT1G22310.2; AT1G22310. [Q9LME6-1]
DR KEGG; ath:AT1G22310; -.
DR Araport; AT1G22310; -.
DR TAIR; locus:2196526; AT1G22310.
DR eggNOG; ENOG502QPIK; Eukaryota.
DR HOGENOM; CLU_533581_0_0_1; -.
DR InParanoid; Q9LME6; -.
DR OMA; NEGQIHY; -.
DR OrthoDB; 945823at2759; -.
DR PhylomeDB; Q9LME6; -.
DR PRO; PR:Q9LME6; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9LME6; baseline and differential.
DR Genevisible; Q9LME6; AT.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0008327; F:methyl-CpG binding; ISS:TAIR.
DR InterPro; IPR017956; AT_hook_DNA-bd_motif.
DR InterPro; IPR016177; DNA-bd_dom_sf.
DR InterPro; IPR037472; MBD8.
DR InterPro; IPR001739; Methyl_CpG_DNA-bd.
DR PANTHER; PTHR37701; PTHR37701; 2.
DR Pfam; PF02178; AT_hook; 2.
DR Pfam; PF01429; MBD; 1.
DR SMART; SM00384; AT_hook; 2.
DR SUPFAM; SSF54171; SSF54171; 1.
DR PROSITE; PS50982; MBD; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; DNA-binding; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..524
FT /note="Methyl-CpG-binding domain-containing protein 8"
FT /id="PRO_0000405284"
FT DOMAIN 334..406
FT /note="MBD"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00338"
FT REGION 45..151
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 116..130
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..173
FT /note="MDDGDLGNNHHNFLGGAGNRLSAESLPLIDTRLLSQSELRALSQCSSLSPSS
FT SASLAASAGGDDDLTPKIDRSVFNESAGSRKQTFLRLRLARHPQPPEEPPSPQRQRDDS
FT SREEQTQVASLLRSLFNVDSNQSKEEEDEGEEELEDNEGQIHYNSYVYQRPNLDSIQNV
FT LIQ -> MSPLVAGNRHFSVSALRATLNHRRNLLRRNVRETILPVKNKRKSRRFSDLCS
FT MSIRIRVKRKKTKERKSSKITK (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14593172"
FT /id="VSP_040660"
SQ SEQUENCE 524 AA; 58669 MW; 00907F932F8D43E8 CRC64;
MDDGDLGNNH HNFLGGAGNR LSAESLPLID TRLLSQSELR ALSQCSSLSP SSSASLAASA
GGDDDLTPKI DRSVFNESAG SRKQTFLRLR LARHPQPPEE PPSPQRQRDD SSREEQTQVA
SLLRSLFNVD SNQSKEEEDE GEEELEDNEG QIHYNSYVYQ RPNLDSIQNV LIQGTSGNKI
KRKRGRPRKI RNPSEENEVL DLTGEASTYV FVDKTSSNLG MVSRVGSSGI SLDSNSVKRK
RGRPPKNKEE IMNLEKRDSA IVNISAFDKE ELVVNLENRE GTIVDLSALA SVSEDPYEEE
LRRITVGLKT KEEILGFLEQ LNGEWVNIGK KKKVVNACDY GGYLPRGWRL MLYIKRKGSN
LLLACRRYIS PDGQQFETCK EVSTYLRSLL ESPSKNQHYY LQSDNKTLGQ QPVIANESLL
GNSDSMDSET MQYLESGRTS SEVFEEAKAV ENGNEADRVK TSLMQKDDNA DFLNGVEDND
DDMKKRDGNM ENLATLSNSE MTKSLPTTTN ELQQYFSSQI NRVQ
