MBF1A_ARATH
ID MBF1A_ARATH Reviewed; 142 AA.
AC Q9SJI8;
DT 18-MAR-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 25-MAY-2022, entry version 123.
DE RecName: Full=Multiprotein-bridging factor 1a;
GN Name=MBF1A; OrderedLocusNames=At2g42680; ORFNames=F14N22.5;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP FUNCTION, TISSUE SPECIFICITY, AND INDUCTION.
RX PubMed=14988493; DOI=10.1093/pcp/pch017;
RA Tsuda K., Tsuji T., Hirose S., Yamazaki K.;
RT "Three Arabidopsis MBF1 homologs with distinct expression profiles play
RT roles as transcriptional co-activators.";
RL Plant Cell Physiol. 45:225-231(2004).
RN [6]
RP TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, AND INDUCTION.
RX PubMed=15451167; DOI=10.1016/j.bbaexp.2004.08.004;
RA Tsuda K., Yamazaki K.;
RT "Structure and expression analysis of three subtypes of Arabidopsis MBF1
RT genes.";
RL Biochim. Biophys. Acta 1680:1-10(2004).
RN [7]
RP SUBCELLULAR LOCATION.
RX PubMed=16283071; DOI=10.1007/s10265-005-0238-y;
RA Sugikawa Y., Ebihara S., Tsuda K., Niwa Y., Yamazaki K.;
RT "Transcriptional coactivator MBF1s from Arabidopsis predominantly localize
RT in nucleolus.";
RL J. Plant Res. 118:431-437(2005).
RN [8]
RP INDUCTION.
RX PubMed=16244138; DOI=10.1104/pp.105.070110;
RA Suzuki N., Rizhsky L., Liang H., Shuman J., Shulaev V., Mittler R.;
RT "Enhanced tolerance to environmental stress in transgenic plants expressing
RT the transcriptional coactivator multiprotein bridging factor 1c.";
RL Plant Physiol. 139:1313-1322(2005).
RN [9]
RP INDUCTION BY SALT STRESS, AND INDUCTION BY PATHOGEN INFECTION.
RX PubMed=17234157; DOI=10.1016/j.bbrc.2006.12.212;
RA Kim M.-J., Lim G.-H., Kim E.-S., Ko C.-B., Yang K.-Y., Jeong J.-A.,
RA Lee M.-C., Kim C.S.;
RT "Abiotic and biotic stress tolerance in Arabidopsis overexpressing the
RT multiprotein bridging factor 1a (MBF1a) transcriptional coactivator gene.";
RL Biochem. Biophys. Res. Commun. 354:440-446(2007).
CC -!- FUNCTION: Transcriptional coactivator that stimulates transcriptional
CC activity by bridging regulatory proteins and TBP, thereby recruiting
CC TBP to promoters occupied by DNA-binding regulators. {ECO:0000250,
CC ECO:0000269|PubMed:14988493}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000269|PubMed:16283071}.
CC -!- TISSUE SPECIFICITY: Expressed in leaves, roots, stems, flowers,
CC siliques and shoots. Detected only in anthers and some seeds in
CC siliques. {ECO:0000269|PubMed:14988493, ECO:0000269|PubMed:15451167}.
CC -!- DEVELOPMENTAL STAGE: Detected in some seeds from 3 days after
CC pollination (dap) to 11 dap. {ECO:0000269|PubMed:15451167}.
CC -!- INDUCTION: Not induced by heat or cold treatments, H(2)O(2),
CC dehydration, abscisic acid, 2,4-D, ACC, methyl jasmonate or salicylic
CC acid. Weak induction by salt stress and pathogen induction.
CC {ECO:0000269|PubMed:14988493, ECO:0000269|PubMed:15451167,
CC ECO:0000269|PubMed:16244138, ECO:0000269|PubMed:17234157}.
CC -!- DOMAIN: The C-terminal domain (75-142) is essential for nucleolar
CC localization.
CC -!- SIMILARITY: Belongs to the MBF1 family. {ECO:0000305}.
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DR EMBL; AC006931; AAD21738.1; -; Genomic_DNA.
DR EMBL; AC007087; AAM15391.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC10155.1; -; Genomic_DNA.
DR EMBL; AF370280; AAK44095.1; -; mRNA.
DR EMBL; AY063014; AAL34188.1; -; mRNA.
DR EMBL; AY088140; AAM65685.1; -; mRNA.
DR PIR; H84856; H84856.
DR RefSeq; NP_565981.1; NM_129829.4.
DR AlphaFoldDB; Q9SJI8; -.
DR SMR; Q9SJI8; -.
DR BioGRID; 4205; 6.
DR IntAct; Q9SJI8; 6.
DR STRING; 3702.AT2G42680.1; -.
DR iPTMnet; Q9SJI8; -.
DR MetOSite; Q9SJI8; -.
DR PaxDb; Q9SJI8; -.
DR PRIDE; Q9SJI8; -.
DR ProteomicsDB; 250823; -.
DR EnsemblPlants; AT2G42680.1; AT2G42680.1; AT2G42680.
DR GeneID; 818868; -.
DR Gramene; AT2G42680.1; AT2G42680.1; AT2G42680.
DR KEGG; ath:AT2G42680; -.
DR Araport; AT2G42680; -.
DR TAIR; locus:2041509; AT2G42680.
DR eggNOG; KOG3398; Eukaryota.
DR HOGENOM; CLU_112609_1_0_1; -.
DR InParanoid; Q9SJI8; -.
DR OMA; NKAHQGT; -.
DR OrthoDB; 1571466at2759; -.
DR PhylomeDB; Q9SJI8; -.
DR PRO; PR:Q9SJI8; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q9SJI8; baseline and differential.
DR Genevisible; Q9SJI8; AT.
DR GO; GO:0005829; C:cytosol; HDA:TAIR.
DR GO; GO:0005730; C:nucleolus; IDA:TAIR.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003729; F:mRNA binding; IDA:TAIR.
DR GO; GO:0003713; F:transcription coactivator activity; IGI:TAIR.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; TAS:TAIR.
DR CDD; cd00093; HTH_XRE; 1.
DR Gene3D; 1.10.260.40; -; 1.
DR InterPro; IPR001387; Cro/C1-type_HTH.
DR InterPro; IPR010982; Lambda_DNA-bd_dom_sf.
DR InterPro; IPR013729; MBF1_N.
DR Pfam; PF01381; HTH_3; 1.
DR Pfam; PF08523; MBF1; 1.
DR SMART; SM00530; HTH_XRE; 1.
DR SUPFAM; SSF47413; SSF47413; 1.
DR PROSITE; PS50943; HTH_CROC1; 1.
PE 2: Evidence at transcript level;
KW Activator; DNA-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..142
FT /note="Multiprotein-bridging factor 1a"
FT /id="PRO_0000325903"
FT DOMAIN 87..141
FT /note="HTH cro/C1-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00257"
FT DNA_BIND 98..117
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00257"
FT REGION 51..77
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 51..68
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 142 AA; 15620 MW; 76A789084B41E97B CRC64;
MAGIGPITQD WEPVVIRKKP ANAAAKRDEK TVNAARRSGA DIETVRKFNA GTNKAASSGT
SLNTKMLDDD TENLTHERVP TELKKAIMQA RTDKKLTQSQ LAQIINEKPQ VIQEYESGKA
IPNQQILSKL ERALGAKLRG KK
