ID   MBF1_KLULA              Reviewed;         150 AA.
AC   Q6CIP4;
DT   16-AUG-2005, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2005, sequence version 2.
DT   25-MAY-2022, entry version 92.
DE   RecName: Full=Multiprotein-bridging factor 1;
GN   Name=MBF1; OrderedLocusNames=KLLA0F25014g;
OS   Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 /
OS   NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Kluyveromyces.
OX   NCBI_TaxID=284590;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37;
RX   PubMed=15229592; DOI=10.1038/nature02579;
RA   Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA   de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA   Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA   Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA   Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA   Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA   Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA   Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA   Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA   Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA   Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA   Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA   Weissenbach J., Wincker P., Souciet J.-L.;
RT   "Genome evolution in yeasts.";
RL   Nature 430:35-44(2004).
CC   -!- FUNCTION: Transcriptional coactivator that stimulates transcriptional
CC       activity by bridging regulatory proteins and TBP, thereby recruiting
CC       TBP to promoters occupied by DNA-binding regulators. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the MBF1 family. {ECO:0000305}.
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DR   EMBL; CR382126; CAG98903.2; -; Genomic_DNA.
DR   RefSeq; XP_456195.2; XM_456195.2.
DR   AlphaFoldDB; Q6CIP4; -.
DR   SMR; Q6CIP4; -.
DR   STRING; 28985.XP_456195.2; -.
DR   EnsemblFungi; CAG98903; CAG98903; KLLA0_F25014g.
DR   GeneID; 2895195; -.
DR   KEGG; kla:KLLA0_F25014g; -.
DR   eggNOG; KOG3398; Eukaryota.
DR   HOGENOM; CLU_112609_0_1_1; -.
DR   InParanoid; Q6CIP4; -.
DR   Proteomes; UP000000598; Chromosome F.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003713; F:transcription coactivator activity; IEA:EnsemblFungi.
DR   GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IEA:EnsemblFungi.
DR   CDD; cd00093; HTH_XRE; 1.
DR   Gene3D; 1.10.260.40; -; 1.
DR   InterPro; IPR001387; Cro/C1-type_HTH.
DR   InterPro; IPR010982; Lambda_DNA-bd_dom_sf.
DR   InterPro; IPR013729; MBF1_N.
DR   Pfam; PF01381; HTH_3; 1.
DR   Pfam; PF08523; MBF1; 1.
DR   SMART; SM00530; HTH_XRE; 1.
DR   SUPFAM; SSF47413; SSF47413; 1.
DR   PROSITE; PS50943; HTH_CROC1; 1.
PE   3: Inferred from homology;
KW   Activator; DNA-binding; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..150
FT                   /note="Multiprotein-bridging factor 1"
FT                   /id="PRO_0000149809"
FT   DOMAIN          85..139
FT                   /note="HTH cro/C1-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00257"
FT   DNA_BIND        96..115
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00257"
FT   REGION          1..29
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   150 AA;  16446 MW;  BB49E23E51082466 CRC64;
     MSEWEPSTVI GRKVRIGGGG PRQQVARTQG QINEARRSGM VLSVDKKYGT TNSKSSPEGQ
     RLTKVDRETD IVKPKKIDVS VGKAIQKGRQ DKNLTQKDLA TKINEKPTIV NDYESGRGIP
     NQQILGKMER ALGIKLRGKN IGEPLGGPKK