ID   MBHL_RHOCA              Reviewed;         597 AA.
AC   P15284;
DT   01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1990, sequence version 1.
DT   03-AUG-2022, entry version 94.
DE   RecName: Full=Uptake hydrogenase large subunit;
DE            EC=1.12.99.6;
DE   AltName: Full=Hydrogenlyase;
DE   AltName: Full=Membrane-bound hydrogenase large subunit;
GN   Name=hupB; Synonyms=hupL;
OS   Rhodobacter capsulatus (Rhodopseudomonas capsulata).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales;
OC   Rhodobacteraceae; Rhodobacter.
OX   NCBI_TaxID=1061;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 33303 / B10;
RX   PubMed=3067084; DOI=10.1007/bf00340186;
RA   Leclerc M., Colbeau A., Cauvin B., Vignais P.M.;
RT   "Cloning and sequencing of the genes encoding the large and the small
RT   subunits of the H2 uptake hydrogenase (hup) of Rhodobacter capsulatus.";
RL   Mol. Gen. Genet. 214:97-107(1988).
RN   [2]
RP   ERRATUM OF PUBMED:3067084, AND SEQUENCE REVISION.
RA   Leclerc M., Colbeau A., Cauvin B., Vignais P.M.;
RL   Mol. Gen. Genet. 215:368-368(1989).
CC   -!- FUNCTION: This enzyme recycles the H(2) produced by nitrogenase to
CC       increase the production of ATP and to protect nitrogenase against
CC       inhibition or damage by O(2) under carbon- or phosphate-limited
CC       conditions.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=A + H2 = AH2; Xref=Rhea:RHEA:12116, ChEBI:CHEBI:13193,
CC         ChEBI:CHEBI:17499, ChEBI:CHEBI:18276; EC=1.12.99.6;
CC   -!- COFACTOR:
CC       Name=Ni(2+); Xref=ChEBI:CHEBI:49786; Evidence={ECO:0000250};
CC       Note=Binds 1 nickel ion per subunit. {ECO:0000250};
CC   -!- SUBUNIT: Heterodimer of a large and a small subunit.
CC   -!- SUBCELLULAR LOCATION: Cell membrane; Peripheral membrane protein.
CC   -!- SIMILARITY: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit
CC       family. {ECO:0000305}.
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DR   EMBL; X13520; CAA31870.1; -; Genomic_DNA.
DR   PIR; S08317; S08317.
DR   AlphaFoldDB; P15284; -.
DR   SMR; P15284; -.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0008901; F:ferredoxin hydrogenase activity; IEA:InterPro.
DR   GO; GO:0033748; F:hydrogenase (acceptor) activity; IEA:UniProtKB-EC.
DR   GO; GO:0016151; F:nickel cation binding; IEA:InterPro.
DR   Gene3D; 1.10.645.10; -; 1.
DR   InterPro; IPR001501; Ni-dep_hyd_lsu.
DR   InterPro; IPR018194; Ni-dep_hyd_lsu_Ni_BS.
DR   InterPro; IPR029014; NiFe-Hase_large.
DR   Pfam; PF00374; NiFeSe_Hases; 1.
DR   SUPFAM; SSF56762; SSF56762; 1.
DR   PROSITE; PS00507; NI_HGENASE_L_1; 1.
DR   PROSITE; PS00508; NI_HGENASE_L_2; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Membrane; Metal-binding; Nickel; Oxidoreductase.
FT   CHAIN           1..597
FT                   /note="Uptake hydrogenase large subunit"
FT                   /id="PRO_0000199716"
FT   BINDING         75
FT                   /ligand="Ni(2+)"
FT                   /ligand_id="ChEBI:CHEBI:49786"
FT                   /evidence="ECO:0000255"
FT   BINDING         78
FT                   /ligand="Ni(2+)"
FT                   /ligand_id="ChEBI:CHEBI:49786"
FT                   /evidence="ECO:0000255"
FT   BINDING         576
FT                   /ligand="Ni(2+)"
FT                   /ligand_id="ChEBI:CHEBI:49786"
FT                   /evidence="ECO:0000255"
FT   BINDING         579
FT                   /ligand="Ni(2+)"
FT                   /ligand_id="ChEBI:CHEBI:49786"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   597 AA;  66046 MW;  C038E25E05EFB9C8 CRC64;
     MTTQTPNGFT LDNAGKRIVV DPVTRIEGHM RCEVNVNDQG IITNAVSTGT MWRGLEVILK
     GRDPRDAWAF TERICGVCTG THALTSVRAV ESALGITIPD NANSIRNMMQ LNLQIHDHIV
     HFYHLHALDW VNPVNALRAD PKATSELQQM VSPSHPLSSP GYFRDVQNRL KKFVESGQLG
     LFKNGYWDNP AYKLPPEADL MATTHYLEAL DLQKEVVKVH TIFGGKNPHP NWLVGGVPCP
     INVDGVGAVG AINMERLNLV SSIIDRCTEF TRNVYLPDLK AIGGFYKEWL YGGGLSGQSV
     LSYGDIPENP NDFSAGQLHL PRGAIINGNL NEVHDVDTTD PEQVQEFVDH SWYDYGEPGM
     GLHPWDGRTE PKFELGPNLK GTRTNIENID EGAKYSWIKA PRWRGNAMEV GPLAATSSVT
     RKGHEDIKNQ VEGLLRDMNL PVSALFSTLG RTAARALEAE YCCRLQKHFF DKLVTNIKNG
     DSSTANVEKW DPSTWPKEAK GVGMTEAPRG ALGHWVKIKD GRIENYQCVV PTTWNGSPRD
     SKGNIGAFEA SLLNTKMERP EEPVEILRTL HSFDPCLACS THVMSAEGAP LTTVKVR