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MBHM_ECOLI
ID   MBHM_ECOLI              Reviewed;         567 AA.
AC   P0ACE0; P37181; Q2M9K1;
DT   22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 2.
DT   03-AUG-2022, entry version 116.
DE   RecName: Full=Hydrogenase-2 large chain;
DE            Short=HYD2;
DE            EC=1.12.99.6;
DE   AltName: Full=Membrane-bound hydrogenase 2 large subunit;
DE   AltName: Full=NiFe hydrogenase;
DE   Flags: Precursor;
GN   Name=hybC; OrderedLocusNames=b2994, JW2962;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=K12 / TG1;
RX   PubMed=8021226; DOI=10.1128/jb.176.14.4416-4423.1994;
RA   Menon N.K., Chatelus C.Y., Dervartanian M., Wendt J.C., Shanmugam K.T.,
RA   Peck H.D. Jr., Przybyla A.E.;
RT   "Cloning, sequencing, and mutational analysis of the hyb operon encoding
RT   Escherichia coli hydrogenase 2.";
RL   J. Bacteriol. 176:4416-4423(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [4]
RP   PROTEIN SEQUENCE OF 2-7.
RX   PubMed=9738917; DOI=10.1046/j.1432-1327.1998.2550746.x;
RA   Sargent F., Ballantine S.P., Rugman P.A., Palmer T., Boxer D.H.;
RT   "Reassignment of the gene encoding the Escherichia coli hydrogenase 2 small
RT   subunit -- identification of a soluble precursor of the small subunit in a
RT   hypB mutant.";
RL   Eur. J. Biochem. 255:746-754(1998).
CC   -!- FUNCTION: This is one of three E.coli hydrogenases synthesized in
CC       response to different physiological conditions. HYD2 is involved in
CC       hydrogen uptake.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=A + H2 = AH2; Xref=Rhea:RHEA:12116, ChEBI:CHEBI:13193,
CC         ChEBI:CHEBI:17499, ChEBI:CHEBI:18276; EC=1.12.99.6;
CC   -!- COFACTOR:
CC       Name=Ni(2+); Xref=ChEBI:CHEBI:49786; Evidence={ECO:0000250};
CC       Note=Binds 1 nickel ion per subunit. {ECO:0000250};
CC   -!- SUBUNIT: Heterodimer of a large and a small subunit.
CC   -!- INTERACTION:
CC       P0ACE0; P0AAM7: hybG; NbExp=2; IntAct=EBI-549849, EBI-562426;
CC   -!- SUBCELLULAR LOCATION: Cell membrane; Peripheral membrane protein.
CC   -!- SIMILARITY: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit
CC       family. {ECO:0000305}.
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DR   EMBL; U09177; AAA21591.1; -; Genomic_DNA.
DR   EMBL; U28377; AAA69161.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC76030.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAE77055.1; -; Genomic_DNA.
DR   PIR; C55516; C55516.
DR   RefSeq; NP_417468.1; NC_000913.3.
DR   RefSeq; WP_000083065.1; NZ_STEB01000001.1.
DR   PDB; 6EHQ; X-ray; 2.20 A; L/M=1-552.
DR   PDB; 6EHS; X-ray; 1.50 A; L/M=1-552.
DR   PDB; 6EN9; X-ray; 1.50 A; L/M=1-567.
DR   PDB; 6G7M; X-ray; 1.71 A; L/M=1-567.
DR   PDB; 6GAM; X-ray; 1.40 A; L/M=1-567.
DR   PDB; 6GAN; X-ray; 1.60 A; L/M=1-567.
DR   PDB; 6SYO; X-ray; 1.25 A; LLL/MMM=1-567.
DR   PDB; 7NEM; X-ray; 1.35 A; L/M=1-567.
DR   PDBsum; 6EHQ; -.
DR   PDBsum; 6EHS; -.
DR   PDBsum; 6EN9; -.
DR   PDBsum; 6G7M; -.
DR   PDBsum; 6GAM; -.
DR   PDBsum; 6GAN; -.
DR   PDBsum; 6SYO; -.
DR   PDBsum; 7NEM; -.
DR   AlphaFoldDB; P0ACE0; -.
DR   SMR; P0ACE0; -.
DR   BioGRID; 4262375; 28.
DR   ComplexPortal; CPX-282; Hydrogenase-2 complex.
DR   DIP; DIP-36022N; -.
DR   IntAct; P0ACE0; 16.
DR   STRING; 511145.b2994; -.
DR   jPOST; P0ACE0; -.
DR   PaxDb; P0ACE0; -.
DR   PRIDE; P0ACE0; -.
DR   EnsemblBacteria; AAC76030; AAC76030; b2994.
DR   EnsemblBacteria; BAE77055; BAE77055; BAE77055.
DR   GeneID; 66673108; -.
DR   GeneID; 945182; -.
DR   KEGG; ecj:JW2962; -.
DR   KEGG; eco:b2994; -.
DR   PATRIC; fig|1411691.4.peg.3735; -.
DR   EchoBASE; EB1749; -.
DR   eggNOG; COG0374; Bacteria.
DR   HOGENOM; CLU_030087_0_0_6; -.
DR   InParanoid; P0ACE0; -.
DR   OMA; AMFRGFE; -.
DR   PhylomeDB; P0ACE0; -.
DR   BioCyc; EcoCyc:HYBC-MON; -.
DR   BioCyc; MetaCyc:HYBC-MON; -.
DR   PRO; PR:P0ACE0; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0044569; C:[Ni-Fe] hydrogenase complex; IC:ComplexPortal.
DR   GO; GO:0031236; C:extrinsic component of periplasmic side of plasma membrane; IDA:EcoCyc.
DR   GO; GO:0005886; C:plasma membrane; IDA:ComplexPortal.
DR   GO; GO:0008901; F:ferredoxin hydrogenase activity; IEA:InterPro.
DR   GO; GO:0033748; F:hydrogenase (acceptor) activity; IEA:UniProtKB-EC.
DR   GO; GO:0016151; F:nickel cation binding; ISM:EcoCyc.
DR   GO; GO:0019645; P:anaerobic electron transport chain; IDA:ComplexPortal.
DR   GO; GO:0009061; P:anaerobic respiration; IDA:ComplexPortal.
DR   GO; GO:0022904; P:respiratory electron transport chain; IEA:UniProt.
DR   Gene3D; 1.10.645.10; -; 1.
DR   InterPro; IPR001501; Ni-dep_hyd_lsu.
DR   InterPro; IPR018194; Ni-dep_hyd_lsu_Ni_BS.
DR   InterPro; IPR029014; NiFe-Hase_large.
DR   Pfam; PF00374; NiFeSe_Hases; 1.
DR   SUPFAM; SSF56762; SSF56762; 1.
DR   PROSITE; PS00507; NI_HGENASE_L_1; 1.
DR   PROSITE; PS00508; NI_HGENASE_L_2; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Cell membrane; Direct protein sequencing; Membrane;
KW   Metal-binding; Nickel; Oxidoreductase; Reference proteome.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000269|PubMed:9738917"
FT   CHAIN           2..552
FT                   /note="Hydrogenase-2 large chain"
FT                   /id="PRO_0000013407"
FT   PROPEP          553..567
FT                   /id="PRO_0000013408"
FT   BINDING         61
FT                   /ligand="Ni(2+)"
FT                   /ligand_id="ChEBI:CHEBI:49786"
FT                   /evidence="ECO:0000255"
FT   BINDING         64
FT                   /ligand="Ni(2+)"
FT                   /ligand_id="ChEBI:CHEBI:49786"
FT                   /evidence="ECO:0000255"
FT   BINDING         546
FT                   /ligand="Ni(2+)"
FT                   /ligand_id="ChEBI:CHEBI:49786"
FT                   /evidence="ECO:0000255"
FT   BINDING         549
FT                   /ligand="Ni(2+)"
FT                   /ligand_id="ChEBI:CHEBI:49786"
FT                   /evidence="ECO:0000255"
FT   SITE            552..553
FT                   /note="Cleavage; by HybD"
FT   STRAND          3..7
FT                   /evidence="ECO:0007829|PDB:6GAM"
FT   STRAND          12..15
FT                   /evidence="ECO:0007829|PDB:6GAM"
FT   STRAND          17..24
FT                   /evidence="ECO:0007829|PDB:6GAM"
FT   STRAND          27..35
FT                   /evidence="ECO:0007829|PDB:6GAM"
FT   HELIX           41..45
FT                   /evidence="ECO:0007829|PDB:6GAM"
FT   HELIX           50..52
FT                   /evidence="ECO:0007829|PDB:6GAM"
FT   HELIX           53..57
FT                   /evidence="ECO:0007829|PDB:6GAM"
FT   HELIX           58..60
FT                   /evidence="ECO:0007829|PDB:6GAM"
FT   STRAND          62..64
FT                   /evidence="ECO:0007829|PDB:6GAM"
FT   HELIX           67..80
FT                   /evidence="ECO:0007829|PDB:6GAM"
FT   HELIX           86..109
FT                   /evidence="ECO:0007829|PDB:6GAM"
FT   TURN            110..112
FT                   /evidence="ECO:0007829|PDB:6GAM"
FT   HELIX           113..115
FT                   /evidence="ECO:0007829|PDB:6GAM"
FT   HELIX           119..122
FT                   /evidence="ECO:0007829|PDB:6GAM"
FT   HELIX           127..133
FT                   /evidence="ECO:0007829|PDB:6GAM"
FT   TURN            134..137
FT                   /evidence="ECO:0007829|PDB:6GAM"
FT   HELIX           145..160
FT                   /evidence="ECO:0007829|PDB:6GAM"
FT   HELIX           165..167
FT                   /evidence="ECO:0007829|PDB:6GAM"
FT   HELIX           181..209
FT                   /evidence="ECO:0007829|PDB:6GAM"
FT   STRAND          210..214
FT                   /evidence="ECO:0007829|PDB:6GAM"
FT   HELIX           237..247
FT                   /evidence="ECO:0007829|PDB:6GAM"
FT   TURN            248..250
FT                   /evidence="ECO:0007829|PDB:6EHQ"
FT   HELIX           251..256
FT                   /evidence="ECO:0007829|PDB:6GAM"
FT   HELIX           258..268
FT                   /evidence="ECO:0007829|PDB:6GAM"
FT   HELIX           270..273
FT                   /evidence="ECO:0007829|PDB:6GAM"
FT   STRAND          282..284
FT                   /evidence="ECO:0007829|PDB:6GAM"
FT   STRAND          287..289
FT                   /evidence="ECO:0007829|PDB:6GAM"
FT   STRAND          291..294
FT                   /evidence="ECO:0007829|PDB:6EHQ"
FT   STRAND          297..299
FT                   /evidence="ECO:0007829|PDB:6GAM"
FT   STRAND          301..305
FT                   /evidence="ECO:0007829|PDB:6GAM"
FT   HELIX           309..311
FT                   /evidence="ECO:0007829|PDB:6GAM"
FT   STRAND          313..315
FT                   /evidence="ECO:0007829|PDB:6GAM"
FT   HELIX           321..326
FT                   /evidence="ECO:0007829|PDB:6GAM"
FT   STRAND          327..330
FT                   /evidence="ECO:0007829|PDB:6GAM"
FT   TURN            332..335
FT                   /evidence="ECO:0007829|PDB:6GAM"
FT   HELIX           344..346
FT                   /evidence="ECO:0007829|PDB:6GAM"
FT   STRAND          363..365
FT                   /evidence="ECO:0007829|PDB:6G7M"
FT   STRAND          367..370
FT                   /evidence="ECO:0007829|PDB:6GAM"
FT   STRAND          376..378
FT                   /evidence="ECO:0007829|PDB:6EHQ"
FT   HELIX           379..388
FT                   /evidence="ECO:0007829|PDB:6GAM"
FT   HELIX           392..409
FT                   /evidence="ECO:0007829|PDB:6GAM"
FT   HELIX           415..418
FT                   /evidence="ECO:0007829|PDB:6GAM"
FT   HELIX           421..451
FT                   /evidence="ECO:0007829|PDB:6GAM"
FT   STRAND          468..477
FT                   /evidence="ECO:0007829|PDB:6GAM"
FT   STRAND          480..489
FT                   /evidence="ECO:0007829|PDB:6GAM"
FT   STRAND          492..499
FT                   /evidence="ECO:0007829|PDB:6GAM"
FT   HELIX           501..506
FT                   /evidence="ECO:0007829|PDB:6GAM"
FT   HELIX           517..522
FT                   /evidence="ECO:0007829|PDB:6GAM"
FT   HELIX           534..542
FT                   /evidence="ECO:0007829|PDB:6GAM"
FT   HELIX           547..551
FT                   /evidence="ECO:0007829|PDB:6GAM"
SQ   SEQUENCE   567 AA;  62491 MW;  A19E6F513390F29E CRC64;
     MSQRITIDPV TRIEGHLRID CEIENGVVSK AWASGTMWRG MEEIVKNRDP RDAWMIVQRI
     CGVCTTTHAL SSVRAAESAL NIDVPVNAQY IRNIILAAHT THDHIVHFYQ LSALDWVDIT
     SALQADPTKA SEMLKGVSTW HLNSPEEFTK VQNKIKDLVA SGQLGIFANG YWGHPAMKLP
     PEVNLIAVAH YLQALECQRD ANRVVALLGG KTPHIQNLAV GGVANPINLD GLGVLNLERL
     MYIKSFIDKL SDFVEQVYKV DTAVIAAFYP EWLTRGKGAV NYLSVPEFPT DSKNGSFLFP
     GGYIENADLS SYRPITSHSD EYLIKGIQES AKHSWYKDEA PQAPWEGTTI PAYDGWSDDG
     KYSWVKSPTF YGKTVEVGPL ANMLVKLAAG RESTQNKLNE IVAIYQKLTG NTLEVAQLHS
     TLGRIIGRTV HCCELQDILQ NQYSALITNI GKGDHTTFVK PNIPATGEFK GVGFLEAPRG
     MLSHWMVIKD GIISNYQAVV PSTWNSGPRN FNDDVGPYEQ SLVGTPVADP NKPLEVVRTI
     HSFDPCMACA VHVVDADGNE VVSVKVL
 
 
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