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MBL1_GIBM7
ID   MBL1_GIBM7              Reviewed;         399 AA.
AC   W7MLD5;
DT   23-FEB-2022, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   03-AUG-2022, entry version 30.
DE   RecName: Full=Gamma-lactamase MBL1 {ECO:0000305};
DE            EC=3.1.1.- {ECO:0000305|PubMed:26808652};
DE   AltName: Full=Fusarium detoxification of benzoxazolinone cluster 1 protein MBL1 {ECO:0000303|PubMed:26808652};
DE            Short=FDB1 cluster protein MBL1 {ECO:0000303|PubMed:26808652};
DE   AltName: Full=Metallo-beta-lactamase 1 {ECO:0000303|PubMed:26808652};
GN   Name=MBL1 {ECO:0000303|PubMed:26808652}; ORFNames=FVEG_08291;
OS   Gibberella moniliformis (strain M3125 / FGSC 7600) (Maize ear and stalk rot
OS   fungus) (Fusarium verticillioides).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Hypocreomycetidae; Hypocreales; Nectriaceae; Fusarium;
OC   Fusarium fujikuroi species complex.
OX   NCBI_TaxID=334819;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=M3125 / FGSC 7600;
RX   PubMed=20237561; DOI=10.1038/nature08850;
RA   Ma L.-J., van der Does H.C., Borkovich K.A., Coleman J.J., Daboussi M.-J.,
RA   Di Pietro A., Dufresne M., Freitag M., Grabherr M., Henrissat B.,
RA   Houterman P.M., Kang S., Shim W.-B., Woloshuk C., Xie X., Xu J.-R.,
RA   Antoniw J., Baker S.E., Bluhm B.H., Breakspear A., Brown D.W.,
RA   Butchko R.A.E., Chapman S., Coulson R., Coutinho P.M., Danchin E.G.J.,
RA   Diener A., Gale L.R., Gardiner D.M., Goff S., Hammond-Kosack K.E.,
RA   Hilburn K., Hua-Van A., Jonkers W., Kazan K., Kodira C.D., Koehrsen M.,
RA   Kumar L., Lee Y.-H., Li L., Manners J.M., Miranda-Saavedra D.,
RA   Mukherjee M., Park G., Park J., Park S.-Y., Proctor R.H., Regev A.,
RA   Ruiz-Roldan M.C., Sain D., Sakthikumar S., Sykes S., Schwartz D.C.,
RA   Turgeon B.G., Wapinski I., Yoder O., Young S., Zeng Q., Zhou S.,
RA   Galagan J., Cuomo C.A., Kistler H.C., Rep M.;
RT   "Comparative genomics reveals mobile pathogenicity chromosomes in
RT   Fusarium.";
RL   Nature 464:367-373(2010).
RN   [2]
RP   FUNCTION.
RX   PubMed=11876429; DOI=10.1094/mpmi.2002.15.2.91;
RA   Glenn A.E., Gold S.E., Bacon C.W.;
RT   "Fdb1 and Fdb2, Fusarium verticillioides loci necessary for detoxification
RT   of preformed antimicrobials from corn.";
RL   Mol. Plant Microbe Interact. 15:91-101(2002).
RN   [3]
RP   FUNCTION.
RX   PubMed=12788712; DOI=10.1128/aem.69.6.3165-3169.2003;
RA   Glenn A.E., Meredith F.I., Morrison W.H. III, Bacon C.W.;
RT   "Identification of intermediate and branch metabolites resulting from
RT   biotransformation of 2-benzoxazolinone by Fusarium verticillioides.";
RL   Appl. Environ. Microbiol. 69:3165-3169(2003).
RN   [4]
RP   FUNCTION.
RX   PubMed=19302487; DOI=10.1111/j.1365-2672.2009.04246.x;
RA   Glenn A.E., Bacon C.W.;
RT   "FDB2 encodes a member of the arylamine N-acetyltransferase family and is
RT   necessary for biotransformation of benzoxazolinones by Fusarium
RT   verticillioides.";
RL   J. Appl. Microbiol. 107:657-671(2009).
RN   [5]
RP   FUNCTION, INDUCTION, DISRUPTION PHENOTYPE, AND PATHWAY.
RX   PubMed=26808652; DOI=10.1371/journal.pone.0147486;
RA   Glenn A.E., Davis C.B., Gao M., Gold S.E., Mitchell T.R., Proctor R.H.,
RA   Stewart J.E., Snook M.E.;
RT   "Two horizontally transferred xenobiotic resistance gene clusters
RT   associated with detoxification of benzoxazolinones by Fusarium species.";
RL   PLoS ONE 11:e0147486-e0147486(2016).
CC   -!- FUNCTION: Gamma-lactamase; part of the Fusarium detoxification of
CC       benzoxazolinone cluster 1 (FDB1) involved in the degradation of
CC       benzoxazolinones produced by the host plant (PubMed:19302487,
CC       PubMed:26808652). Maize, wheat, and rye produce the 2 benzoxazinone
CC       phytoanticipins 2,4-dihy-droxy-7-methoxy-1,4-benzoxazin-3-one (DIMBOA)
CC       and 2,4-dihydroxy-1,4-benzoxazin-3-one (DIBOA) that, due to their
CC       inherent instability once released, spontaneously degrade to the more
CC       stable corresponding benzoxazolinones, 6-methoxy-2-benzoxazolinone
CC       (MBOA) and 2-benzoxazolinone (BOA), respectively (PubMed:11876429). The
CC       first step in the detoxification of benzoxazolinones involves the
CC       hydrolysis of the cyclic ester bond of benzoxazolinones by the FDB1
CC       cluster gamma-lactamase MBL1 to aminophenols (PubMed:26808652,
CC       PubMed:12788712). MBL1 is able to convert BOA into 2-aminophenol (2-
CC       AP), as well as MBOA into 5-methoxy-2-aminophenol (2-AMP)
CC       (PubMed:26808652, PubMed:12788712). The FDB2 cluster N-
CC       malonyltransferase FDB2/NAT1 then metabolizes aminophenols via N-
CC       malonylation to non-toxic malonamic acids (PubMed:19302487,
CC       PubMed:12788712). FDB2/NAT1 converts 2-AP into N-(2-hydroxyphenyl)
CC       malonamic acid (HPMA) and 2-AMP into N-(2-hydroxy-4-methoxyphenyl)
CC       malonamic acid (HMPMA) (PubMed:19302487, PubMed:12788712). The
CC       duplicated dienlactone hydrolases DLH1 and DLH2 may provide redundant
CC       function for hydrolyzing the lactone moiety in the BOA molecule
CC       (Probable). The roles of the amidases and other enzymes encoded by the
CC       2 FDB clusters have not been identified so far (Probable).
CC       {ECO:0000269|PubMed:11876429, ECO:0000269|PubMed:12788712,
CC       ECO:0000269|PubMed:19302487, ECO:0000269|PubMed:26808652,
CC       ECO:0000305|PubMed:26808652}.
CC   -!- PATHWAY: Xenobiotic degradation. {ECO:0000269|PubMed:12788712,
CC       ECO:0000269|PubMed:26808652}.
CC   -!- INDUCTION: Expression is induced in response to 2-benzoxasolinone (BOA)
CC       exposure. {ECO:0000269|PubMed:26808652}.
CC   -!- DISRUPTION PHENOTYPE: Impairs the ability to of metabolize 2-
CC       benzoxazolinone (BOA) and thus growth BOA-amended media.
CC       {ECO:0000269|PubMed:26808652}.
CC   -!- MISCELLANEOUS: Fusarium verticillioides possesses 2 unlinked loci, FDB1
CC       and FDB2, necessary for detoxification of antimicrobial compounds
CC       produced by maize, including 2-benzoxazolinone (BOA) (PubMed:26808652).
CC       The FDB2 cluster arose as a duplication of the FDB1 cluster with
CC       rearrangement and expansion by incorporating additional genes
CC       (Probable). {ECO:0000269|PubMed:26808652, ECO:0000305|PubMed:26808652}.
CC   -!- SIMILARITY: Belongs to the metallo-beta-lactamase superfamily.
CC       {ECO:0000305}.
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DR   EMBL; DS022252; EWG48579.1; -; Genomic_DNA.
DR   RefSeq; XP_018754770.1; XM_018897183.1.
DR   EnsemblFungi; FVEG_08291T0; FVEG_08291T0; FVEG_08291.
DR   GeneID; 30066033; -.
DR   KEGG; fvr:FVEG_08291; -.
DR   VEuPathDB; FungiDB:FVEG_08291; -.
DR   eggNOG; ENOG502S1A6; Eukaryota.
DR   HOGENOM; CLU_030571_1_0_1; -.
DR   OMA; FEAYDFY; -.
DR   OrthoDB; 1139836at2759; -.
DR   Proteomes; UP000009096; Chromosome 10.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   Gene3D; 3.60.15.10; -; 1.
DR   InterPro; IPR001279; Metallo-B-lactamas.
DR   InterPro; IPR036866; RibonucZ/Hydroxyglut_hydro.
DR   Pfam; PF00753; Lactamase_B; 1.
DR   SMART; SM00849; Lactamase_B; 1.
DR   SUPFAM; SSF56281; SSF56281; 1.
PE   2: Evidence at transcript level;
KW   Hydrolase; Metal-binding; Reference proteome; Zinc.
FT   CHAIN           1..399
FT                   /note="Gamma-lactamase MBL1"
FT                   /id="PRO_0000454592"
FT   BINDING         147
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250|UniProtKB:Q7B8B9"
FT   BINDING         149
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250|UniProtKB:Q7B8B9"
FT   BINDING         151
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250|UniProtKB:Q7B8B9"
FT   BINDING         152
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250|UniProtKB:Q7B8B9"
FT   BINDING         234
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250|UniProtKB:Q7B8B9"
FT   BINDING         258
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250|UniProtKB:Q7B8B9"
FT   BINDING         258
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250|UniProtKB:Q7B8B9"
FT   BINDING         351
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250|UniProtKB:Q7B8B9"
SQ   SEQUENCE   399 AA;  44602 MW;  10A6FED93CDB00A9 CRC64;
     MLTSPLRQDH LVPETLMADA SPIIRPETPP DLHIPDSQAT VKVSIIDTTS YMSNFPMWAF
     LDPLMPGHEE MKGCDFSFII EHPKSGNKYD TLLFDLGVRK DWENLPTPFV QGIKAGGCKI
     EVQKDVATIL KDNGRDLSEV GGIIWSHWHF DHVGDAQTFP GTTDVIVGPG FKKAHVPAWP
     TDPESHIDEK AWQGRTLREI DFAAEEGKGL KIGQFDALDF YGDGSFYVLN TPGHTIGHLS
     ALARTTVDPP TFIFMGGDIA HQGGEFRPTP YMPLPAEISP NPFNRILPRP ALTCPGEIFV
     EIHRNKSRTE PFFDPTTAEG AWHHCAHEAK RSIDKLTEFD AYENIFPVIA HDNSLYGVID
     VYPKPANDWF AKSWKDKTRW GWLNEFDPSF AGLAKASEP
 
 
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