MBL_DROME
ID MBL_DROME Reviewed; 297 AA.
AC O16011; O16008; O16009; O16010; Q86BE8; Q9V803; Q9V804;
DT 16-NOV-2001, integrated into UniProtKB/Swiss-Prot.
DT 02-MAY-2002, sequence version 2.
DT 03-AUG-2022, entry version 171.
DE RecName: Full=Protein muscleblind;
DE AltName: Full=Protein mindmelt;
GN Name=mbl; Synonyms=mm; ORFNames=CG33197;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS A; B AND D), FUNCTION, SUBCELLULAR
RP LOCATION, AND TISSUE SPECIFICITY.
RC STRAIN=Oregon-R; TISSUE=Imaginal disk;
RX PubMed=9334280; DOI=10.1242/dev.124.21.4321;
RA Begemann G., Paricio N., Artero R., Kiss I., Perez-Alonso M., Mlodzik M.;
RT "Muscleblind, a gene required for photoreceptor differentiation in
RT Drosophila, encodes novel nuclear Cys3His-type zinc-finger-containing
RT proteins.";
RL Development 124:4321-4331(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION, AND ALTERNATIVE SPLICING.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
CC -!- FUNCTION: Required for terminal differentiation of photoreceptor cells.
CC Vital for embryonic development. {ECO:0000269|PubMed:9334280}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:9334280}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Comment=Additional isoforms seem to exist.;
CC Name=B;
CC IsoId=O16011-1; Sequence=Displayed;
CC Name=A;
CC IsoId=O16011-2; Sequence=VSP_006425, VSP_006426;
CC Name=D; Synonyms=Begemann-D;
CC IsoId=O16011-3; Sequence=VSP_006427, VSP_006428;
CC -!- TISSUE SPECIFICITY: Expressed in embryonic muscle cells.
CC {ECO:0000269|PubMed:9334280}.
CC -!- SIMILARITY: Belongs to the muscleblind family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC01948.1; Type=Miscellaneous discrepancy; Note=Aberrant splicing.; Evidence={ECO:0000305};
CC Sequence=AAC47758.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AF001422; AAC01948.1; ALT_SEQ; mRNA.
DR EMBL; AF001536; AAC01949.1; -; mRNA.
DR EMBL; AF001625; AAC47757.1; -; mRNA.
DR EMBL; AF001626; AAC47758.1; ALT_FRAME; mRNA.
DR EMBL; AE013599; AAO41357.1; -; Genomic_DNA.
DR EMBL; AE013599; AAO41358.1; -; Genomic_DNA.
DR EMBL; AE013599; AAO41359.1; -; Genomic_DNA.
DR RefSeq; NP_788390.1; NM_176210.2. [O16011-3]
DR RefSeq; NP_788391.1; NM_176211.2. [O16011-1]
DR RefSeq; NP_788392.1; NM_176212.2. [O16011-2]
DR AlphaFoldDB; O16011; -.
DR SMR; O16011; -.
DR BioGRID; 62642; 62.
DR DIP; DIP-17614N; -.
DR IntAct; O16011; 19.
DR STRING; 7227.FBpp0297556; -.
DR PaxDb; O16011; -.
DR EnsemblMetazoa; FBtr0086991; FBpp0086143; FBgn0265487. [O16011-2]
DR EnsemblMetazoa; FBtr0086992; FBpp0086144; FBgn0265487. [O16011-1]
DR EnsemblMetazoa; FBtr0086994; FBpp0086146; FBgn0265487. [O16011-3]
DR GeneID; 36945; -.
DR KEGG; dme:Dmel_CG33197; -.
DR UCSC; CG33197-RA; d. melanogaster. [O16011-1]
DR CTD; 36945; -.
DR FlyBase; FBgn0265487; mbl.
DR VEuPathDB; VectorBase:FBgn0265487; -.
DR eggNOG; KOG2494; Eukaryota.
DR GeneTree; ENSGT00950000182897; -.
DR HOGENOM; CLU_937717_0_0_1; -.
DR InParanoid; O16011; -.
DR PhylomeDB; O16011; -.
DR BioGRID-ORCS; 36945; 0 hits in 3 CRISPR screens.
DR ChiTaRS; mbl; fly.
DR GenomeRNAi; 36945; -.
DR PRO; PR:O16011; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0265487; Expressed in brain and 21 other tissues.
DR ExpressionAtlas; O16011; baseline and differential.
DR Genevisible; O16011; DM.
DR GO; GO:0005737; C:cytoplasm; IDA:FlyBase.
DR GO; GO:0031673; C:H zone; IDA:FlyBase.
DR GO; GO:0005654; C:nucleoplasm; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0048471; C:perinuclear region of cytoplasm; IDA:FlyBase.
DR GO; GO:0030018; C:Z disc; IDA:FlyBase.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0003676; F:nucleic acid binding; NAS:UniProtKB.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0006915; P:apoptotic process; IDA:FlyBase.
DR GO; GO:0001751; P:compound eye photoreceptor cell differentiation; IMP:FlyBase.
DR GO; GO:0001654; P:eye development; IMP:FlyBase.
DR GO; GO:0046716; P:muscle cell cellular homeostasis; IMP:FlyBase.
DR GO; GO:0007517; P:muscle organ development; IMP:UniProtKB.
DR GO; GO:0000381; P:regulation of alternative mRNA splicing, via spliceosome; IMP:FlyBase.
DR GO; GO:0045924; P:regulation of female receptivity; IMP:FlyBase.
DR GO; GO:0010468; P:regulation of gene expression; IMP:FlyBase.
DR GO; GO:0050896; P:response to stimulus; IEA:UniProtKB-KW.
DR GO; GO:0042052; P:rhabdomere development; IMP:FlyBase.
DR GO; GO:0007601; P:visual perception; IEA:UniProtKB-KW.
DR InterPro; IPR000571; Znf_CCCH.
DR SMART; SM00356; ZnF_C3H1; 2.
DR PROSITE; PS50103; ZF_C3H1; 2.
PE 2: Evidence at transcript level;
KW Alternative splicing; Developmental protein; Metal-binding; Nucleus;
KW Reference proteome; Repeat; Sensory transduction; Vision; Zinc;
KW Zinc-finger.
FT CHAIN 1..297
FT /note="Protein muscleblind"
FT /id="PRO_0000089177"
FT ZN_FING 18..46
FT /note="C3H1-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00723"
FT ZN_FING 52..80
FT /note="C3H1-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00723"
FT VAR_SEQ 180..243
FT /note="TSPLAAHHHQQQQQLQHQLNNINNNNNHSTAGAAATSTTATTTTNNAAAAAA
FT AAAAAAAAAVMG -> MDVKTVGSFYYDNFQFSGMVPFKRPAAEKSGIPVYQPGATAYQ
FT QLMQPYVPVSFTGHPPGVPRF (in isoform D)"
FT /evidence="ECO:0000303|PubMed:9334280"
FT /id="VSP_006427"
FT VAR_SEQ 180..203
FT /note="TSPLAAHHHQQQQQLQHQLNNINN -> VSFGLMPTTIYISRYMFMYPMKSI
FT (in isoform A)"
FT /evidence="ECO:0000303|PubMed:9334280"
FT /id="VSP_006425"
FT VAR_SEQ 204..297
FT /note="Missing (in isoform A)"
FT /evidence="ECO:0000303|PubMed:9334280"
FT /id="VSP_006426"
FT VAR_SEQ 244..297
FT /note="Missing (in isoform D)"
FT /evidence="ECO:0000303|PubMed:9334280"
FT /id="VSP_006428"
FT CONFLICT 16
FT /note="S -> W (in Ref. 1; AAC01948)"
FT /evidence="ECO:0000305"
FT CONFLICT 189
FT /note="Missing (in Ref. 1; AAC47758)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 297 AA; 32330 MW; 37D430C798D91185 CRC64;
MANVVNMNSL LNGKDSRWLQ LEVCREFQRN KCSRQDTECK FAHPPANVEV QNGKVTACYD
SIKGRCNRDK PPCKYFHPPQ HLKDQLLING RNHLALKNAL MQQMGIAPGQ PVISGQVPAV
ATNPYLTGIP ANSYSPYYTT GHLVPALLGP DPVTSQLGPV VPQTVQVAQQ KIPRSDRLET
SPLAAHHHQQ QQQLQHQLNN INNNNNHSTA GAAATSTTAT TTTNNAAAAA AAAAAAAAAA
VMGHHTLEVG KKRAADTTDM FPLVFFCSFP SPCVFAFLNC SIFGFLRLWF SLFNLRH
