MBNL3_MOUSE
ID MBNL3_MOUSE Reviewed; 342 AA.
AC Q8R003;
DT 06-DEC-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 03-AUG-2022, entry version 138.
DE RecName: Full=Muscleblind-like protein 3;
DE AltName: Full=Cys3His CCG1-required protein;
DE AltName: Full=Muscleblind-like X-linked protein;
DE AltName: Full=Protein MCHCR;
GN Name=Mbnl3; Synonyms=Chcr, Mbxl;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND FUNCTION.
RC TISSUE=Myoblast;
RX PubMed=12297108; DOI=10.1006/dbio.2002.0798;
RA Squillace R.M., Chenault D.M., Wang E.H.;
RT "Inhibition of muscle differentiation by the novel muscleblind-related
RT protein CHCR.";
RL Dev. Biol. 250:218-230(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=NMRI; TISSUE=Mammary gland;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Mediates pre-mRNA alternative splicing regulation. Acts
CC either as activator or repressor of splicing on specific pre-mRNA
CC targets. Inhibits cardiac troponin-T (TNNT2) pre-mRNA exon inclusion
CC but induces insulin receptor (IR) pre-mRNA exon inclusion in muscle.
CC Antagonizes the alternative splicing activity pattern of CELF proteins
CC (By similarity). Could inhibit terminal muscle differentiation, acting
CC at approximately the time of myogenin induction. {ECO:0000250,
CC ECO:0000269|PubMed:12297108}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}. Cytoplasm {ECO:0000250}.
CC Note=Mostly nuclear. {ECO:0000250}.
CC -!- DEVELOPMENTAL STAGE: High expression in proliferating myoblasts is
CC strongly reduced in differentiated muscle cells.
CC -!- SIMILARITY: Belongs to the muscleblind family. {ECO:0000305}.
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DR EMBL; AF466292; AAL87669.1; -; mRNA.
DR EMBL; AB077700; BAB85650.1; -; mRNA.
DR EMBL; AJ427920; CAD20871.1; -; mRNA.
DR EMBL; BC057157; AAH57157.1; -; mRNA.
DR CCDS; CCDS40969.1; -.
DR RefSeq; NP_598924.1; NM_134163.5.
DR AlphaFoldDB; Q8R003; -.
DR SMR; Q8R003; -.
DR STRING; 10090.ENSMUSP00000110526; -.
DR iPTMnet; Q8R003; -.
DR PhosphoSitePlus; Q8R003; -.
DR EPD; Q8R003; -.
DR MaxQB; Q8R003; -.
DR PaxDb; Q8R003; -.
DR PRIDE; Q8R003; -.
DR ProteomicsDB; 287321; -.
DR Antibodypedia; 446; 157 antibodies from 25 providers.
DR DNASU; 171170; -.
DR Ensembl; ENSMUST00000114876; ENSMUSP00000110526; ENSMUSG00000036109.
DR GeneID; 171170; -.
DR KEGG; mmu:171170; -.
DR UCSC; uc009tdt.1; mouse.
DR CTD; 55796; -.
DR MGI; MGI:2444912; Mbnl3.
DR VEuPathDB; HostDB:ENSMUSG00000036109; -.
DR eggNOG; KOG2494; Eukaryota.
DR GeneTree; ENSGT00950000182897; -.
DR HOGENOM; CLU_053536_0_0_1; -.
DR InParanoid; Q8R003; -.
DR OMA; IECKYAH; -.
DR OrthoDB; 1543798at2759; -.
DR PhylomeDB; Q8R003; -.
DR TreeFam; TF321931; -.
DR BioGRID-ORCS; 171170; 0 hits in 58 CRISPR screens.
DR ChiTaRS; Mbnl3; mouse.
DR PRO; PR:Q8R003; -.
DR Proteomes; UP000000589; Chromosome X.
DR RNAct; Q8R003; protein.
DR Bgee; ENSMUSG00000036109; Expressed in placenta labyrinth and 160 other tissues.
DR ExpressionAtlas; Q8R003; baseline and differential.
DR Genevisible; Q8R003; MM.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005654; C:nucleoplasm; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR GO; GO:0045662; P:negative regulation of myoblast differentiation; IDA:MGI.
DR GO; GO:0000381; P:regulation of alternative mRNA splicing, via spliceosome; IBA:GO_Central.
DR GO; GO:0043484; P:regulation of RNA splicing; ISS:UniProtKB.
DR GO; GO:0008380; P:RNA splicing; IEA:UniProtKB-KW.
DR InterPro; IPR041367; Znf-CCCH_4.
DR InterPro; IPR000571; Znf_CCCH.
DR Pfam; PF18044; zf-CCCH_4; 1.
DR SMART; SM00356; ZnF_C3H1; 4.
DR PROSITE; PS50103; ZF_C3H1; 4.
PE 2: Evidence at transcript level;
KW Cytoplasm; Developmental protein; Metal-binding; mRNA processing;
KW mRNA splicing; Nucleus; Reference proteome; Repeat; Zinc; Zinc-finger.
FT CHAIN 1..342
FT /note="Muscleblind-like protein 3"
FT /id="PRO_0000089181"
FT ZN_FING 14..42
FT /note="C3H1-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00723"
FT ZN_FING 48..74
FT /note="C3H1-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00723"
FT ZN_FING 174..202
FT /note="C3H1-type 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00723"
FT ZN_FING 210..236
FT /note="C3H1-type 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00723"
FT REGION 316..342
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 342 AA; 37570 MW; 493C38EB6D33DB17 CRC64;
MTPVNVALIR DTKWLTLEVC REFQRGTCSR ADAECRFAHP PRVCHVENGR VVACFDSLKG
RCTRENCKYL HPPPHLKSQL EVNGRNNLIQ QKTAAAMFAQ HMQLMLQNAQ MSSLASFPMN
PSLAANPAMA FNPYMTHPGM GLVPAELLPN GPVLISGNPP LALPGVPGPK PIRTDRLEVC
REFQRGNCTR GESECRYAHP TDVSMIEVTD NSVTICMDYI KGRCSREKCK YFHPPPHLQA
KLRAAHHQMN HSAANAMALP HGALQLIPKR SALDKANGAT PVFNPSVFHC QQALANMQIP
QQAFIPTVPM MHGATPSTVS TATPPASNVP YVPTTTGNQL KY
