MBN_DROME
ID MBN_DROME Reviewed; 653 AA.
AC P52302; Q59E19; Q8MS87; Q9VRU6;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 02-FEB-2004, sequence version 2.
DT 03-AUG-2022, entry version 128.
DE RecName: Full=Protein lethal(3)malignant blood neoplasm 1;
DE AltName: Full=Mbn(83);
GN Name=l(3)mbn; Synonyms=l(3)mbn-1; ORFNames=CG12755;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION, AND
RP TISSUE SPECIFICITY.
RC STRAIN=Oregon-R; TISSUE=Larva;
RX PubMed=8174791; DOI=10.1006/dbio.1994.1126;
RA Konrad L., Becker G., Schmidt A., Kloeckner T., Kaufer-Stillger G.,
RA Dreschers S., Edstroem J.-E., Gateff E.;
RT "Cloning, structure, cellular localization, and possible function of the
RT tumor suppressor gene lethal(3)malignant blood neoplasm-1 of Drosophila
RT melanogaster.";
RL Dev. Biol. 163:98-111(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-298.
RC STRAIN=Berkeley; TISSUE=Larva, and Pupae;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
CC -!- FUNCTION: Required for differentiation of the phagocytic blood-cell
CC type, the plasmatocyte. {ECO:0000269|PubMed:8174791}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:8174791}.
CC Cytoplasm, perinuclear region {ECO:0000269|PubMed:8174791}. Note=Mainly
CC around the nucleus.
CC -!- TISSUE SPECIFICITY: Blood cells and other tissues.
CC {ECO:0000269|PubMed:8174791}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAM50879.1; Type=Miscellaneous discrepancy; Note=Intron retention.; Evidence={ECO:0000305};
CC Sequence=CAA87654.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; Z47722; CAA87654.1; ALT_FRAME; Genomic_DNA.
DR EMBL; AE014296; AAN12238.2; -; Genomic_DNA.
DR EMBL; AY119019; AAM50879.1; ALT_SEQ; mRNA.
DR PIR; S57844; S57844.
DR RefSeq; NP_729143.2; NM_168157.2.
DR AlphaFoldDB; P52302; -.
DR BioGRID; 64156; 3.
DR STRING; 7227.FBpp0076770; -.
DR PaxDb; P52302; -.
DR PRIDE; P52302; -.
DR DNASU; 38701; -.
DR EnsemblMetazoa; FBtr0077062; FBpp0076770; FBgn0002440.
DR GeneID; 38701; -.
DR KEGG; dme:Dmel_CG12755; -.
DR FlyBase; FBgn0002440; l(3)mbn.
DR VEuPathDB; VectorBase:FBgn0002440; -.
DR eggNOG; ENOG502S1RB; Eukaryota.
DR InParanoid; P52302; -.
DR OrthoDB; 1361828at2759; -.
DR PhylomeDB; P52302; -.
DR BioGRID-ORCS; 38701; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 38701; -.
DR PRO; PR:P52302; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0002440; Expressed in thoracico-abdominal ganglion (Drosophila) and 5 other tissues.
DR ExpressionAtlas; P52302; baseline and differential.
DR Genevisible; P52302; DM.
DR GO; GO:0062129; C:chitin-based extracellular matrix; ISM:FlyBase.
DR GO; GO:0048471; C:perinuclear region of cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005886; C:plasma membrane; ISS:FlyBase.
DR GO; GO:0008010; F:structural constituent of chitin-based larval cuticle; ISM:FlyBase.
DR GO; GO:0040003; P:chitin-based cuticle development; ISM:FlyBase.
DR InterPro; IPR000618; Insect_cuticle.
DR Pfam; PF00379; Chitin_bind_4; 1.
DR PROSITE; PS51155; CHIT_BIND_RR_2; 2.
PE 2: Evidence at transcript level;
KW Cytoplasm; Reference proteome; Repeat.
FT CHAIN 1..653
FT /note="Protein lethal(3)malignant blood neoplasm 1"
FT /id="PRO_0000196182"
FT DOMAIN 28..91
FT /note="Chitin-binding type R&R 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00497"
FT REPEAT 307..327
FT /note="1-1"
FT REPEAT 328..348
FT /note="1-2"
FT REPEAT 433..455
FT /note="2-1"
FT REPEAT 456..475
FT /note="2-2"
FT REPEAT 476..497
FT /note="2-3"
FT REPEAT 498..515
FT /note="2-4"
FT REPEAT 516..533
FT /note="2-5"
FT REPEAT 534..551
FT /note="2-6"
FT REPEAT 552..568
FT /note="2-7"
FT DOMAIN 572..640
FT /note="Chitin-binding type R&R 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00497"
FT REGION 307..348
FT /note="2 X 21 AA repeats"
FT REGION 425..448
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 433..568
FT /note="7 X approximate tandem repeats, Gly/Ser-rich"
FT CONFLICT 56
FT /note="E -> Q (in Ref. 1; CAA87654)"
FT /evidence="ECO:0000305"
FT CONFLICT 67
FT /note="H -> Q (in Ref. 1; CAA87654)"
FT /evidence="ECO:0000305"
FT CONFLICT 72
FT /note="A -> P (in Ref. 1; CAA87654)"
FT /evidence="ECO:0000305"
FT CONFLICT 109
FT /note="P -> H (in Ref. 1; CAA87654)"
FT /evidence="ECO:0000305"
FT CONFLICT 189
FT /note="T -> A (in Ref. 1; CAA87654)"
FT /evidence="ECO:0000305"
FT CONFLICT 208
FT /note="I -> L (in Ref. 1; CAA87654)"
FT /evidence="ECO:0000305"
FT CONFLICT 226
FT /note="T -> R (in Ref. 1; CAA87654)"
FT /evidence="ECO:0000305"
FT CONFLICT 422
FT /note="V -> A (in Ref. 1; CAA87654)"
FT /evidence="ECO:0000305"
FT CONFLICT 529
FT /note="P -> A (in Ref. 1; CAA87654)"
FT /evidence="ECO:0000305"
FT CONFLICT 536
FT /note="L -> S (in Ref. 1; CAA87654)"
FT /evidence="ECO:0000305"
FT CONFLICT 556
FT /note="F -> L (in Ref. 1; CAA87654)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 653 AA; 67144 MW; D2303B9FE417F81E CRC64;
MSLKLMAFVC ALLLLCTLTH VLSAATTVRP YKFGFTIDEQ QHRAEKRDER GIIMGEFGFI
TADGIYHVTV YATDEEGKFR IISMKSYPYA GPVGSKSVPV TTTPKVLLPA APVALPKYNF
NSEACSGCFL KKSPPKTEIR TLSQPLAPVQ PGKPDGSSDI GLNVQLPFRE SIAQTVASRL
GLLQDTLQTS NTNTNAPNTK TIELGLNIAM STYYTTKNAV TGHVSTQTSN SQTPSANTKI
DYNVGVVEKA SPPVYRPLNI RLNEDVMRQA ITYGNTIPGH VPLPNQPLVE TSLLPASQVK
IFALDGNAKV PLASNIQSVA QHPNAGLNAK VPLASNIQSV AQHPNAGLNA KVPLASNIQS
VAQHPNAGLK NINRSGVSSA KTLANTKTRP PHTFNPHQTP LLSSATAPGI SGVTANTPTG
NVPSNGGGIA AGKAPGNPQA GGSGGIIGAG APGGRKVSAG GIGSGSAIGG VSGGSKASGN
GGAIGSGSAI GGGATGSKAS GFGFGSNIGG GVSGSKPSGF GSESKIGGPD SGSKALGFGS
GSKIGGGITG TKASGFGGEI GSGRGSASSA TGDLYKFKYI LDYNGHEETG GRNGDKQGSY
FAIGEDAVQR TIEYIANEFG FQPHVSWRKL DAKEALPEEN SLKHYEFKWF NQE
