MBS1_ARATH
ID MBS1_ARATH Reviewed; 105 AA.
AC Q9M8S0;
DT 18-JUL-2018, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 148.
DE RecName: Full=Protein METHYLENE BLUE SENSITIVITY 1 {ECO:0000303|PubMed:24151292};
GN Name=MBS1 {ECO:0000303|PubMed:24151292};
GN OrderedLocusNames=At3g02790 {ECO:0000312|Araport:AT3G02790};
GN ORFNames=F13E7.27 {ECO:0000312|EMBL:AAF26981.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP FUNCTION, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION, AND INDUCTION BY HIGH
RP LIGHT.
RC STRAIN=cv. Columbia;
RX PubMed=24151292; DOI=10.1105/tpc.113.117390;
RA Shao N., Duan G.Y., Bock R.;
RT "A mediator of singlet oxygen responses in Chlamydomonas reinhardtii and
RT Arabidopsis identified by a luciferase-based genetic screen in algal
RT cells.";
RL Plant Cell 25:4209-4226(2013).
RN [5]
RP FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, SUBCELLULAR LOCATION,
RP AND INDUCTION BY BETA-CYCLOCITRAL.
RC STRAIN=cv. Columbia;
RX PubMed=27813110; DOI=10.1111/pce.12856;
RA Shumbe L., D'Alessandro S., Shao N., Chevalier A., Ksas B., Bock R.,
RA Havaux M.;
RT "METHYLENE BLUE SENSITIVITY 1 (MBS1) is required for acclimation of
RT Arabidopsis to singlet oxygen and acts downstream of beta-cyclocitral.";
RL Plant Cell Environ. 40:216-226(2017).
CC -!- FUNCTION: Required for acclimation to reactive oxygen species (ROS)
CC responses downstream of beta-cyclocitral (beta-cc) or mediated by
CC dihydroactinidiolide, including singlet oxygen 1O(2) detoxification
CC reactions, especially upon light-mediated photooxidative stress, and
CC leading to programmed cell death (PubMed:24151292, PubMed:27813110).
CC Prevents leaf senescence (PubMed:24151292). Involved in cold
CC acclimation (PubMed:27813110). {ECO:0000269|PubMed:24151292,
CC ECO:0000269|PubMed:27813110}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:24151292,
CC ECO:0000269|PubMed:27813110}. Cytoplasm {ECO:0000269|PubMed:24151292,
CC ECO:0000269|PubMed:27813110}. Cytoplasm, Stress granule
CC {ECO:0000269|PubMed:24151292}. Note=Moves from homogeneous distribution
CC in the cytosol to discrete spots (e.g. stress granules (SGs) and
CC processing bodies (PBs)) in response to singlet oxygen 1O(2) exposure
CC (PubMed:24151292). Accumulates in nucleus after beta-cyclocitral (beta-
CC cc) treatment (PubMed:27813110). {ECO:0000269|PubMed:24151292,
CC ECO:0000269|PubMed:27813110}.
CC -!- TISSUE SPECIFICITY: Mainly expressed in the epidermis.
CC {ECO:0000269|PubMed:27813110}.
CC -!- INDUCTION: By high light (PubMed:24151292). Triggered by the carotenoid
CC metabolite beta-cyclocitral (beta-cc) (PubMed:27813110).
CC {ECO:0000269|PubMed:24151292, ECO:0000269|PubMed:27813110}.
CC -!- DISRUPTION PHENOTYPE: Normal plants under standard growth conditions,
CC but severe symptoms of photooxidative damage under excess light due to
CC a deficient induction of singlet oxygen 1O(2) detoxification reactions,
CC thus leading to pale leaf phenotype, early senescence and lack of
CC anthocyanin accumulation (PubMed:24151292, PubMed:27813110). Increased
CC susceptibility to low temperatures and high photon flux density (PFD)
CC conditions. Increased lipid peroxidation in response to beta-
CC cyclocitral (beta-cc) associated with reduced induction of reactive
CC oxygen species (ROS) responsive genes (e.g. AAA, LTI30, ZAT12 and
CC WRKY40). Altered induction of gene (e.g. AAA, OXI1, CAT2, APX1 and
CC RD20) expression in response to the lactone dihydroactinidiolide
CC (PubMed:27813110). Accumulation of singlet oxygen 1O(2) in chloroplasts
CC in response to light stress. Early senescence of the older leaves.
CC Plants lacking both MBS1 and MBS2 exhibit premature leaf senescence
CC (PubMed:24151292). {ECO:0000269|PubMed:24151292,
CC ECO:0000269|PubMed:27813110}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AC018363; AAF26981.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE73860.1; -; Genomic_DNA.
DR EMBL; AF361599; AAK32767.1; -; mRNA.
DR EMBL; AY058229; AAL15403.1; -; mRNA.
DR RefSeq; NP_566182.1; NM_111147.3.
DR AlphaFoldDB; Q9M8S0; -.
DR IntAct; Q9M8S0; 3.
DR STRING; 3702.AT3G02790.1; -.
DR MetOSite; Q9M8S0; -.
DR PaxDb; Q9M8S0; -.
DR PRIDE; Q9M8S0; -.
DR ProteomicsDB; 238851; -.
DR EnsemblPlants; AT3G02790.1; AT3G02790.1; AT3G02790.
DR GeneID; 820985; -.
DR Gramene; AT3G02790.1; AT3G02790.1; AT3G02790.
DR KEGG; ath:AT3G02790; -.
DR Araport; AT3G02790; -.
DR TAIR; locus:2075397; AT3G02790.
DR eggNOG; ENOG502S122; Eukaryota.
DR HOGENOM; CLU_160241_1_0_1; -.
DR InParanoid; Q9M8S0; -.
DR OMA; IHHESKQ; -.
DR OrthoDB; 1620202at2759; -.
DR PhylomeDB; Q9M8S0; -.
DR PRO; PR:Q9M8S0; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9M8S0; differential.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0010494; C:cytoplasmic stress granule; IDA:UniProtKB.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IPI:TAIR.
DR GO; GO:0071452; P:cellular response to singlet oxygen; IMP:TAIR.
DR GO; GO:0009631; P:cold acclimation; IMP:UniProtKB.
DR GO; GO:1900055; P:regulation of leaf senescence; IMP:UniProtKB.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; TAS:TAIR.
DR GO; GO:0009644; P:response to high light intensity; IMP:UniProtKB.
DR GO; GO:0080183; P:response to photooxidative stress; IMP:UniProtKB.
DR InterPro; IPR045230; MBS1/2-like.
DR PANTHER; PTHR21213; PTHR21213; 1.
PE 2: Evidence at transcript level;
KW Cytoplasm; Nucleus; Reference proteome; Stress response.
FT CHAIN 1..105
FT /note="Protein METHYLENE BLUE SENSITIVITY 1"
FT /id="PRO_0000444900"
FT REGION 26..46
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 105 AA; 11278 MW; 4F882E77711B5514 CRC64;
MTGKAKPKKH TAKEIQAKID AALTNRGGGK AGIADRTGKE KGGHAKYECP HCKITAPGLK
TMQIHHESKH PNIIYEESKL VNLHAVLAPV AESKPKPGIR GSLKK
