MBS2_ARATH
ID MBS2_ARATH Reviewed; 104 AA.
AC Q9FFD8;
DT 18-JUL-2018, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 25-MAY-2022, entry version 150.
DE RecName: Full=Protein METHYLENE BLUE SENSITIVITY 2 {ECO:0000303|PubMed:24151292};
GN Name=MBS2 {ECO:0000303|PubMed:24151292};
GN OrderedLocusNames=At5g16470 {ECO:0000312|Araport:AT5G16470};
GN ORFNames=MQK4.20 {ECO:0000312|EMBL:BAB09614.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9330910; DOI=10.1093/dnares/4.3.215;
RA Sato S., Kotani H., Nakamura Y., Kaneko T., Asamizu E., Fukami M.,
RA Miyajima N., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. I. Sequence
RT features of the 1.6 Mb regions covered by twenty physically assigned P1
RT clones.";
RL DNA Res. 4:215-230(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP FUNCTION, DISRUPTION PHENOTYPE, INDUCTION BY SENESCENCE, AND SUBCELLULAR
RP LOCATION.
RC STRAIN=cv. Columbia;
RX PubMed=24151292; DOI=10.1105/tpc.113.117390;
RA Shao N., Duan G.Y., Bock R.;
RT "A mediator of singlet oxygen responses in Chlamydomonas reinhardtii and
RT Arabidopsis identified by a luciferase-based genetic screen in algal
RT cells.";
RL Plant Cell 25:4209-4226(2013).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19376835; DOI=10.1104/pp.109.138677;
RA Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A.,
RA Grossmann J., Gruissem W., Baginsky S.;
RT "Large-scale Arabidopsis phosphoproteome profiling reveals novel
RT chloroplast kinase substrates and phosphorylation networks.";
RL Plant Physiol. 150:889-903(2009).
CC -!- FUNCTION: Required for acclimation to reactive oxygen species (ROS)
CC responses downstream of beta-cyclocitral, including singlet oxygen
CC 1O(2) detoxification reactions, especially upon light-mediated
CC photooxidative stress, and leading to programmed cell death (By
CC similarity). Prevents leaf senescence (PubMed:24151292).
CC {ECO:0000250|UniProtKB:Q9M8S0, ECO:0000269|PubMed:24151292}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:24151292}. Cytoplasm
CC {ECO:0000269|PubMed:24151292}. Cytoplasm, Stress granule
CC {ECO:0000250|UniProtKB:Q9M8S0}. Note=Moves from homogeneous
CC distribution in the cytosol to discrete spots (e.g. stress granules
CC (SGs) and processing bodies (PBs)) in response to singlet oxygen 1O(2)
CC exposure. {ECO:0000250|UniProtKB:Q9M8S0}.
CC -!- INDUCTION: Accumulates during senescence.
CC {ECO:0000269|PubMed:24151292}.
CC -!- DISRUPTION PHENOTYPE: Plants lacking both MBS1 and MBS2 exhibit
CC premature leaf senescence. {ECO:0000269|PubMed:24151292}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AB005242; BAB09614.1; -; Genomic_DNA.
DR EMBL; CP002688; AED92297.1; -; Genomic_DNA.
DR EMBL; CP002688; ANM70670.1; -; Genomic_DNA.
DR EMBL; BT003166; AAO24598.1; -; mRNA.
DR EMBL; AK228163; BAF00119.1; -; mRNA.
DR RefSeq; NP_001332259.1; NM_001343451.1.
DR RefSeq; NP_197151.1; NM_121652.4.
DR AlphaFoldDB; Q9FFD8; -.
DR SMR; Q9FFD8; -.
DR STRING; 3702.AT5G16470.1; -.
DR iPTMnet; Q9FFD8; -.
DR PaxDb; Q9FFD8; -.
DR PRIDE; Q9FFD8; -.
DR ProteomicsDB; 238852; -.
DR EnsemblPlants; AT5G16470.1; AT5G16470.1; AT5G16470.
DR EnsemblPlants; AT5G16470.2; AT5G16470.2; AT5G16470.
DR GeneID; 831508; -.
DR Gramene; AT5G16470.1; AT5G16470.1; AT5G16470.
DR Gramene; AT5G16470.2; AT5G16470.2; AT5G16470.
DR KEGG; ath:AT5G16470; -.
DR Araport; AT5G16470; -.
DR TAIR; locus:2171422; AT5G16470.
DR eggNOG; ENOG502S122; Eukaryota.
DR HOGENOM; CLU_160241_1_0_1; -.
DR InParanoid; Q9FFD8; -.
DR OMA; PKMPYEE; -.
DR OrthoDB; 1620202at2759; -.
DR PhylomeDB; Q9FFD8; -.
DR PRO; PR:Q9FFD8; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FFD8; baseline and differential.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0010494; C:cytoplasmic stress granule; ISS:UniProtKB.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0009536; C:plastid; HDA:TAIR.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0071452; P:cellular response to singlet oxygen; IMP:TAIR.
DR GO; GO:0010150; P:leaf senescence; IEP:UniProtKB.
DR GO; GO:1900055; P:regulation of leaf senescence; IMP:UniProtKB.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; TAS:TAIR.
DR GO; GO:0080183; P:response to photooxidative stress; ISS:UniProtKB.
DR InterPro; IPR045230; MBS1/2-like.
DR PANTHER; PTHR21213; PTHR21213; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Nucleus; Reference proteome; Stress response.
FT CHAIN 1..104
FT /note="Protein METHYLENE BLUE SENSITIVITY 2"
FT /id="PRO_0000444901"
FT REGION 1..46
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 64..104
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 64..79
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 104 AA; 11239 MW; 04C41509FC8DBA91 CRC64;
MTGKAKPKKH TAKELQAKAD AALTNRGGGK AGLADRTGKE KGGHAKYECP HCKITVPDLK
TMQIHHESKH PKLTYEEPRN LHEALAAPAE SSKPKPGIRG SLKK
