ID   MBTG_MYCPA              Reviewed;         428 AA.
AC   Q73XY8;
DT   28-NOV-2006, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 89.
DE   RecName: Full=L-lysine N6-monooxygenase MbtG;
DE            EC=1.14.13.59;
DE   AltName: Full=Lysine 6-N-hydroxylase;
DE   AltName: Full=Lysine N6-hydroxylase;
DE   AltName: Full=Lysine-N-oxygenase;
DE   AltName: Full=Mycobactin synthase protein G;
DE   Flags: Precursor;
GN   Name=mbtG; OrderedLocusNames=MAP_2170c;
OS   Mycolicibacterium paratuberculosis (strain ATCC BAA-968 / K-10)
OS   (Mycobacterium paratuberculosis).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium avium complex (MAC).
OX   NCBI_TaxID=262316;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-968 / K-10;
RX   PubMed=16116077; DOI=10.1073/pnas.0505662102;
RA   Li L., Bannantine J.P., Zhang Q., Amonsin A., May B.J., Alt D., Banerji N.,
RA   Kanjilal S., Kapur V.;
RT   "The complete genome sequence of Mycobacterium avium subspecies
RT   paratuberculosis.";
RL   Proc. Natl. Acad. Sci. U.S.A. 102:12344-12349(2005).
CC   -!- FUNCTION: Flavoprotein monooxygenase required for N-hydroxylation of
CC       the two acylated lysine residues during mycobactin assembly, thus
CC       producing the hydroxamate groups necessary for iron sequestration. Is
CC       also able, but less efficiently, to hydroxylate L-lysine (non acylated)
CC       in vitro (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=L-lysine + NADPH + O2 = H2O + N(6)-hydroxy-L-lysine + NADP(+);
CC         Xref=Rhea:RHEA:23228, ChEBI:CHEBI:15377, ChEBI:CHEBI:15379,
CC         ChEBI:CHEBI:32551, ChEBI:CHEBI:57783, ChEBI:CHEBI:57820,
CC         ChEBI:CHEBI:58349; EC=1.14.13.59;
CC   -!- COFACTOR:
CC       Name=FAD; Xref=ChEBI:CHEBI:57692; Evidence={ECO:0000250};
CC   -!- PATHWAY: Siderophore biosynthesis; mycobactin biosynthesis.
CC   -!- SIMILARITY: Belongs to the lysine N(6)-hydroxylase/L-ornithine N(5)-
CC       oxygenase family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AE016958; AAS04487.1; -; Genomic_DNA.
DR   RefSeq; WP_003878341.1; NC_002944.2.
DR   AlphaFoldDB; Q73XY8; -.
DR   SMR; Q73XY8; -.
DR   STRING; 262316.MAP_2170c; -.
DR   EnsemblBacteria; AAS04487; AAS04487; MAP_2170c.
DR   KEGG; mpa:MAP_2170c; -.
DR   PATRIC; fig|262316.17.peg.2307; -.
DR   eggNOG; COG3486; Bacteria.
DR   HOGENOM; CLU_052650_0_0_11; -.
DR   OMA; QFAEWVD; -.
DR   UniPathway; UPA00011; -.
DR   Proteomes; UP000000580; Chromosome.
DR   GO; GO:0047091; F:L-lysine 6-monooxygenase (NADPH) activity; IEA:UniProtKB-EC.
DR   GO; GO:0044249; P:cellular biosynthetic process; IEA:UniProt.
DR   GO; GO:1901566; P:organonitrogen compound biosynthetic process; IEA:UniProt.
DR   Gene3D; 3.50.50.60; -; 1.
DR   InterPro; IPR036188; FAD/NAD-bd_sf.
DR   InterPro; IPR025700; Lys/Orn_oxygenase.
DR   Pfam; PF13434; K_oxygenase; 1.
DR   SUPFAM; SSF51905; SSF51905; 2.
PE   3: Inferred from homology;
KW   FAD; Flavoprotein; Monooxygenase; NADP; Oxidoreductase; Reference proteome;
KW   Signal.
FT   SIGNAL          1..20
FT                   /evidence="ECO:0000255"
FT   CHAIN           21..428
FT                   /note="L-lysine N6-monooxygenase MbtG"
FT                   /id="PRO_0000261310"
SQ   SEQUENCE   428 AA;  46532 MW;  44C8EE39A4AFA801 CRC64;
     MSTLAILGAG AKAVAVAAKA SVLRDMGVEV PDVVAVERIG VAANWQASGG WTDGAHRLGT
     SPEKDVGFPY RSALVPRRNA ELDERMTRYS WQSYLIATAS FAEWIDRGRP APTHRRWSQY
     LSWVADHVGM TVVHGEVEQL AVTGDRWALH THETTVHADA LMITGPGQAE KSLLPGNPRM
     LSIAQFWDRA ANHDRISAER VAVIGGGETA AAMLNELFRH RVSSITVISP QATLFTRGEG
     YFENSLFSDP TNWPALTLAE RRDALARTDR GVFSSSVQEA LLADDRIHHL RGRVTHAVGV
     QGQIRLTLST NRGSENLETV HGFDLVIDGS GADSLWFAPL FSQEALDLLE LGLGGPLSSE
     RLQEAIGYDL AVTDVTPKLF LPNLSGLTQG PGFPNLSCLG LLSDRVLGST LGPTNYPARR
     RHDERQPL