ID   MBTL_MYCTU              Reviewed;          84 AA.
AC   P9WQF1; L0T6K8; P63452; Q11014;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   03-AUG-2022, entry version 44.
DE   RecName: Full=Acyl carrier protein MbtL;
DE            Short=ACP;
DE   AltName: Full=Mycobactin synthase protein L;
GN   Name=mbtL; OrderedLocusNames=Rv1344; ORFNames=MTCY02B10.08;
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   INDUCTION.
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=12065475; DOI=10.1128/iai.70.7.3371-3381.2002;
RA   Rodriguez G.M., Voskuil M.I., Gold B., Schoolnik G.K., Smith I.;
RT   "IdeR, an essential gene in Mycobacterium tuberculosis: role of IdeR in
RT   iron-dependent gene expression, iron metabolism, and oxidative stress
RT   response.";
RL   Infect. Immun. 70:3371-3381(2002).
RN   [3]
RP   FUNCTION, ROLE IN MYCOBACTIN BIOSYNTHESIS, AND PHOSPHOPANTETHEINYLATION.
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=16461464; DOI=10.1073/pnas.0507924103;
RA   Krithika R., Marathe U., Saxena P., Ansari M.Z., Mohanty D., Gokhale R.S.;
RT   "A genetic locus required for iron acquisition in Mycobacterium
RT   tuberculosis.";
RL   Proc. Natl. Acad. Sci. U.S.A. 103:2069-2074(2006).
RN   [4]
RP   PHOSPHOPANTETHEINYLATION AT SER-41, AND MUTAGENESIS OF SER-41.
RX   PubMed=28203522; DOI=10.1002/2211-5463.12140;
RA   Jung J., Bashiri G., Johnston J.M., Baker E.N.;
RT   "Mass spectral determination of phosphopantetheinylation specificity for
RT   carrier proteins in Mycobacterium tuberculosis.";
RL   FEBS Open Bio 6:1220-1226(2016).
CC   -!- FUNCTION: Acyl carrier protein involved in the formation of acyl-S-ACP
CC       intermediates within the mycobactin biosynthesis process. The aliphatic
CC       chains carried by ACP are subsequently transferred on to the mycobactin
CC       core by MbtK. {ECO:0000269|PubMed:16461464}.
CC   -!- PATHWAY: Siderophore biosynthesis; mycobactin biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- INDUCTION: Induced by iron starvation conditions. Transcriptionally
CC       repressed by IdeR and iron. {ECO:0000269|PubMed:12065475}.
CC   -!- PTM: 4'-phosphopantetheine is transferred from CoA to a specific serine
CC       of apo-ACP by PptT, leading to the activated holo-ACP form.
CC       {ECO:0000269|PubMed:28203522}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CCP44102.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; AL123456; CCP44102.1; ALT_INIT; Genomic_DNA.
DR   PIR; G70739; G70739.
DR   RefSeq; NP_215860.1; NC_000962.3.
DR   AlphaFoldDB; P9WQF1; -.
DR   SMR; P9WQF1; -.
DR   STRING; 83332.Rv1344; -.
DR   PaxDb; P9WQF1; -.
DR   DNASU; 886848; -.
DR   GeneID; 886848; -.
DR   KEGG; mtu:Rv1344; -.
DR   PATRIC; fig|83332.12.peg.1506; -.
DR   TubercuList; Rv1344; -.
DR   eggNOG; COG0236; Bacteria.
DR   OMA; DMNVDVR; -.
DR   BioCyc; MetaCyc:G185E-5523-MON; -.
DR   UniPathway; UPA00011; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0000036; F:acyl carrier activity; IDA:MTBBASE.
DR   GO; GO:0019540; P:catechol-containing siderophore biosynthetic process; IDA:MTBBASE.
DR   Gene3D; 1.10.1200.10; -; 1.
DR   InterPro; IPR036736; ACP-like_sf.
DR   InterPro; IPR009081; PP-bd_ACP.
DR   Pfam; PF00550; PP-binding; 1.
DR   SUPFAM; SSF47336; SSF47336; 1.
DR   PROSITE; PS50075; CARRIER; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; Phosphopantetheine; Phosphoprotein; Reference proteome.
FT   CHAIN           1..84
FT                   /note="Acyl carrier protein MbtL"
FT                   /id="PRO_0000180269"
FT   DOMAIN          6..81
FT                   /note="Carrier"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT   MOD_RES         41
FT                   /note="O-(pantetheine 4'-phosphoryl)serine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00258,
FT                   ECO:0000269|PubMed:28203522"
FT   MUTAGEN         41
FT                   /note="S->A: Lack of phosphopantetheinylation."
FT                   /evidence="ECO:0000269|PubMed:28203522"
SQ   SEQUENCE   84 AA;  8969 MW;  9B50302AC4A3E82C CRC64;
     MTSSPSTVST TLLSILRDDL NIDLTRVTPD ARLVDDVGLD SVAFAVGMVA IEERLGVALS
     EEELLTCDTV GELEAAIAAK YRDE