ID   MC69_COLGM              Reviewed;          56 AA.
AC   E3QFA4;
DT   11-DEC-2019, integrated into UniProtKB/Swiss-Prot.
DT   11-JAN-2011, sequence version 1.
DT   25-MAY-2022, entry version 25.
DE   RecName: Full=Secreted virulence factor CLU5a {ECO:0000303|PubMed:31599055};
DE   AltName: Full=Pathogenicity cluster 5 protein a {ECO:0000303|PubMed:31599055};
DE   Flags: Precursor;
GN   Name=CLU5a {ECO:0000303|PubMed:31599055}; ORFNames=GLRG_04686;
OS   Colletotrichum graminicola (strain M1.001 / M2 / FGSC 10212) (Maize
OS   anthracnose fungus) (Glomerella graminicola).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Hypocreomycetidae; Glomerellales; Glomerellaceae; Colletotrichum;
OC   Colletotrichum graminicola species complex.
OX   NCBI_TaxID=645133;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND INDUCTION.
RC   STRAIN=M1.001 / M2 / FGSC 10212;
RX   PubMed=22885923; DOI=10.1038/ng.2372;
RA   O'Connell R.J., Thon M.R., Hacquard S., Amyotte S.G., Kleemann J.,
RA   Torres M.F., Damm U., Buiate E.A., Epstein L., Alkan N., Altmueller J.,
RA   Alvarado-Balderrama L., Bauser C.A., Becker C., Birren B.W., Chen Z.,
RA   Choi J., Crouch J.A., Duvick J.P., Farman M.A., Gan P., Heiman D.,
RA   Henrissat B., Howard R.J., Kabbage M., Koch C., Kracher B., Kubo Y.,
RA   Law A.D., Lebrun M.-H., Lee Y.-H., Miyara I., Moore N., Neumann U.,
RA   Nordstroem K., Panaccione D.G., Panstruga R., Place M., Proctor R.H.,
RA   Prusky D., Rech G., Reinhardt R., Rollins J.A., Rounsley S., Schardl C.L.,
RA   Schwartz D.C., Shenoy N., Shirasu K., Sikhakolli U.R., Stueber K.,
RA   Sukno S.A., Sweigard J.A., Takano Y., Takahara H., Trail F.,
RA   van der Does H.C., Voll L.M., Will I., Young S., Zeng Q., Zhang J.,
RA   Zhou S., Dickman M.B., Schulze-Lefert P., Ver Loren van Themaat E.,
RA   Ma L.-J., Vaillancourt L.J.;
RT   "Lifestyle transitions in plant pathogenic Colletotrichum fungi deciphered
RT   by genome and transcriptome analyses.";
RL   Nat. Genet. 44:1060-1065(2012).
RN   [2]
RP   INDUCTION.
RX   PubMed=26956617; DOI=10.1186/s12864-016-2546-0;
RA   Torres M.F., Ghaffari N., Buiate E.A., Moore N., Schwartz S., Johnson C.D.,
RA   Vaillancourt L.J.;
RT   "A Colletotrichum graminicola mutant deficient in the establishment of
RT   biotrophy reveals early transcriptional events in the maize anthracnose
RT   disease interaction.";
RL   BMC Genomics 17:202-202(2016).
RN   [3]
RP   FUNCTION, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION, INDUCTION, DISULFIDE
RP   BOND, AND SUBUNIT.
RX   PubMed=31599055; DOI=10.1111/1462-2920.14819;
RA   Eisermann I., Weihmann F., Krijger J.J., Kroeling C., Hause G., Menzel M.,
RA   Pienkny S., Kiesow A., Deising H.B., Wirsel S.G.R.;
RT   "Two genes in a pathogenicity gene cluster encoding secreted proteins are
RT   required for appressorial penetration and infection of the maize
RT   anthracnose fungus Colletotrichum graminicola.";
RL   Environ. Microbiol. 21:4773-4791(2019).
CC   -!- FUNCTION: Secreted protein required for appressorial penetration of
CC       intact host epidermal cells and for pathogenicit, but not for
CC       subsequent biotrophic and necrotrophic colonization of leaves.
CC       {ECO:0000269|PubMed:31599055}.
CC   -!- SUBUNIT: Homodimer; disulfide-linked (Probable). Dimerization can
CC       possibly extend to multimerisation (Probable).
CC       {ECO:0000305|PubMed:31599055}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:31599055}.
CC   -!- INDUCTION: Expressed at all stages of pathogenesis.
CC       {ECO:0000269|PubMed:22885923, ECO:0000269|PubMed:26956617,
CC       ECO:0000269|PubMed:31599055}.
CC   -!- DISRUPTION PHENOTYPE: Impairs pathogenicity (PubMed:31599055). Forms
CC       pressurised appressoria but is hampered in forming penetration pores
CC       and fails to differentiate a penetration peg (PubMed:31599055).
CC       {ECO:0000269|PubMed:31599055}.
CC   -!- SIMILARITY: Belongs to the MC69 virulence factor family. {ECO:0000305}.
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DR   EMBL; GG697345; EFQ29542.1; -; Genomic_DNA.
DR   RefSeq; XP_008093562.1; XM_008095371.1.
DR   AlphaFoldDB; E3QFA4; -.
DR   EnsemblFungi; EFQ29542; EFQ29542; GLRG_04686.
DR   GeneID; 24410051; -.
DR   VEuPathDB; FungiDB:GLRG_04686; -.
DR   eggNOG; ENOG502SV3A; Eukaryota.
DR   HOGENOM; CLU_205482_0_0_1; -.
DR   OrthoDB; 1646962at2759; -.
DR   Proteomes; UP000008782; Unassembled WGS sequence.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
PE   1: Evidence at protein level;
KW   Disulfide bond; Reference proteome; Secreted; Signal; Virulence.
FT   SIGNAL          1..18
FT                   /evidence="ECO:0000255"
FT   CHAIN           19..56
FT                   /note="Secreted virulence factor CLU5a"
FT                   /id="PRO_5003180371"
FT   DISULFID        41
FT                   /note="Interchain (with C-51)"
FT                   /evidence="ECO:0000305|PubMed:31599055"
FT   DISULFID        51
FT                   /note="Interchain (with C-41)"
FT                   /evidence="ECO:0000305|PubMed:31599055"
SQ   SEQUENCE   56 AA;  5682 MW;  13698F007C818552 CRC64;
     MKVSLTLLAT LCASLASAGV VITPVHQDQV VPAAQKVAGD CFFGVVTPQG CAPLRT