ID   MC69_COLOR              Reviewed;          54 AA.
AC   H7CE70; N4UPH1;
DT   11-DEC-2019, integrated into UniProtKB/Swiss-Prot.
DT   16-MAY-2012, sequence version 1.
DT   25-MAY-2022, entry version 13.
DE   RecName: Full=Secreted virulence factor MC69 {ECO:0000303|PubMed:22589729};
DE   Flags: Precursor;
GN   Name=MC69 {ECO:0000303|PubMed:22589729}; ORFNames=Cob_13056;
OS   Colletotrichum orbiculare (strain 104-T / ATCC 96160 / CBS 514.97 / LARS
OS   414 / MAFF 240422) (Cucumber anthracnose fungus) (Colletotrichum
OS   lagenarium).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Hypocreomycetidae; Glomerellales; Glomerellaceae; Colletotrichum;
OC   Colletotrichum orbiculare species complex.
OX   NCBI_TaxID=1213857;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, DISRUPTION PHENOTYPE, AND
RP   INDUCTION.
RC   STRAIN=104-T / ATCC 96160 / CBS 514.97 / LARS 414 / MAFF 240422;
RX   PubMed=22589729; DOI=10.1371/journal.ppat.1002711;
RA   Saitoh H., Fujisawa S., Mitsuoka C., Ito A., Hirabuchi A., Ikeda K.,
RA   Irieda H., Yoshino K., Yoshida K., Matsumura H., Tosa Y., Win J.,
RA   Kamoun S., Takano Y., Terauchi R.;
RT   "Large-scale gene disruption in Magnaporthe oryzae identifies MC69, a
RT   secreted protein required for infection by monocot and dicot fungal
RT   pathogens.";
RL   PLoS Pathog. 8:E1002711-E1002711(2012).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=104-T / ATCC 96160 / CBS 514.97 / LARS 414 / MAFF 240422;
RX   PubMed=23252678; DOI=10.1111/nph.12085;
RA   Gan P., Ikeda K., Irieda H., Narusaka M., O'Connell R.J., Narusaka Y.,
RA   Takano Y., Kubo Y., Shirasu K.;
RT   "Comparative genomic and transcriptomic analyses reveal the hemibiotrophic
RT   stage shift of Colletotrichum fungi.";
RL   New Phytol. 197:1236-1249(2013).
RN   [3]
RP   FUNCTION, DISRUPTION PHENOTYPE, AND SUBCELLULAR LOCATION.
RX   PubMed=24850852; DOI=10.1105/tpc.113.120600;
RA   Irieda H., Maeda H., Akiyama K., Hagiwara A., Saitoh H., Uemura A.,
RA   Terauchi R., Takano Y.;
RT   "Colletotrichum orbiculare secretes virulence effectors to a biotrophic
RT   interface at the primary hyphal neck via exocytosis coupled with SEC22-
RT   mediated traffic.";
RL   Plant Cell 26:2265-2281(2014).
CC   -!- FUNCTION: Secreted protein required for appressorial penetration of
CC       intact host epidermal cells and for pathogenicity.
CC       {ECO:0000269|PubMed:22589729, ECO:0000269|PubMed:24850852}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:24850852}.
CC       Note=Secreted exocytosis coupled with SEC22-mediated traffic and
CC       accumulates in a ring-like region around the neck of the biotrophic
CC       primary hyphae. {ECO:0000269|PubMed:24850852}.
CC   -!- INDUCTION: Expressed in the plant infection stage.
CC       {ECO:0000269|PubMed:22589729}.
CC   -!- DISRUPTION PHENOTYPE: Impairs pathogenicity (PubMed:22589729,
CC       PubMed:24850852). Leads to clear reduction in lesion development on
CC       cucumber leaves (PubMed:22589729). {ECO:0000269|PubMed:22589729,
CC       ECO:0000269|PubMed:24850852}.
CC   -!- SIMILARITY: Belongs to the MC69 virulence factor family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=ENH77638.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AB669186; BAL70272.1; -; Genomic_DNA.
DR   EMBL; KB726077; ENH77638.1; ALT_SEQ; Genomic_DNA.
DR   AlphaFoldDB; H7CE70; -.
DR   EnsemblFungi; ENH77638; ENH77638; Cob_13056.
DR   eggNOG; ENOG502SV3A; Eukaryota.
DR   HOGENOM; CLU_1835044_0_0_1; -.
DR   OrthoDB; 1646962at2759; -.
DR   PHI-base; PHI:3123; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
PE   2: Evidence at transcript level;
KW   Disulfide bond; Secreted; Signal; Virulence.
FT   SIGNAL          1..18
FT                   /evidence="ECO:0000255"
FT   CHAIN           19..54
FT                   /note="Secreted virulence factor MC69"
FT                   /id="PRO_5003608307"
FT   DISULFID        38..48
FT                   /evidence="ECO:0000250|UniProtKB:G5EI17"
SQ   SEQUENCE   54 AA;  5563 MW;  2D6F5B71CF25B878 CRC64;
     MKFTLALLTT LCASLASAGV VITPVRPNQV IPANSGDCFF GVVTPQGCAP LRKS