ID   MC69_MAGO7              Reviewed;          54 AA.
AC   G5EI17;
DT   11-DEC-2019, integrated into UniProtKB/Swiss-Prot.
DT   14-DEC-2011, sequence version 1.
DT   25-MAY-2022, entry version 35.
DE   RecName: Full=Secreted virulence factor MC69 {ECO:0000303|PubMed:22589729};
DE   Flags: Precursor;
GN   Name=MC69 {ECO:0000303|PubMed:22589729}; ORFNames=MGCH7_ch7g606, MGG_02848;
OS   Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958) (Rice blast
OS   fungus) (Pyricularia oryzae).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Sordariomycetidae; Magnaporthales; Pyriculariaceae; Pyricularia.
OX   NCBI_TaxID=242507;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=70-15 / ATCC MYA-4617 / FGSC 8958;
RX   PubMed=15846337; DOI=10.1038/nature03449;
RA   Dean R.A., Talbot N.J., Ebbole D.J., Farman M.L., Mitchell T.K.,
RA   Orbach M.J., Thon M.R., Kulkarni R., Xu J.-R., Pan H., Read N.D.,
RA   Lee Y.-H., Carbone I., Brown D., Oh Y.Y., Donofrio N., Jeong J.S.,
RA   Soanes D.M., Djonovic S., Kolomiets E., Rehmeyer C., Li W., Harding M.,
RA   Kim S., Lebrun M.-H., Bohnert H., Coughlan S., Butler J., Calvo S.E.,
RA   Ma L.-J., Nicol R., Purcell S., Nusbaum C., Galagan J.E., Birren B.W.;
RT   "The genome sequence of the rice blast fungus Magnaporthe grisea.";
RL   Nature 434:980-986(2005).
RN   [2]
RP   FUNCTION, INDUCTION, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION,
RP   MUTAGENESIS OF CYS-36 AND CYS-46, AND DISULFIDE BOND.
RX   PubMed=22589729; DOI=10.1371/journal.ppat.1002711;
RA   Saitoh H., Fujisawa S., Mitsuoka C., Ito A., Hirabuchi A., Ikeda K.,
RA   Irieda H., Yoshino K., Yoshida K., Matsumura H., Tosa Y., Win J.,
RA   Kamoun S., Takano Y., Terauchi R.;
RT   "Large-scale gene disruption in Magnaporthe oryzae identifies MC69, a
RT   secreted protein required for infection by monocot and dicot fungal
RT   pathogens.";
RL   PLoS Pathog. 8:E1002711-E1002711(2012).
CC   -!- FUNCTION: Secreted protein required for appressorial penetration of
CC       intact host epidermal cells and for pathogenicity.
CC       {ECO:0000269|PubMed:22589729}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:22589729}.
CC       Note=Accumulates in an interfacial structure named the biotrophic
CC       interfacial complex (BIC) but is not translocated into rice cells.
CC       {ECO:0000269|PubMed:22589729}.
CC   -!- INDUCTION: Expressed in conidia an all stages of infection.
CC       {ECO:0000269|PubMed:22589729}.
CC   -!- DISRUPTION PHENOTYPE: Impairs pathogenicity (PubMed:22589729). Exhibits
CC       a defect in appressorium-mediated penetration in rice leaf sheath cells
CC       but neither in conidial germination nor appressorium formation
CC       (PubMed:22589729). {ECO:0000269|PubMed:22589729}.
CC   -!- SIMILARITY: Belongs to the MC69 virulence factor family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; CM000230; EAQ71199.1; -; Genomic_DNA.
DR   EMBL; CM001237; EHA46146.1; -; Genomic_DNA.
DR   RefSeq; XP_003720889.1; XM_003720841.1.
DR   AlphaFoldDB; G5EI17; -.
DR   EnsemblFungi; MGG_02848T0; MGG_02848T0; MGG_02848.
DR   GeneID; 2682401; -.
DR   KEGG; mgr:MGG_02848; -.
DR   VEuPathDB; FungiDB:MGG_02848; -.
DR   eggNOG; ENOG502RPF6; Eukaryota.
DR   HOGENOM; CLU_205482_0_0_1; -.
DR   InParanoid; G5EI17; -.
DR   OMA; QAGDCFF; -.
DR   OrthoDB; 1646962at2759; -.
DR   PHI-base; PHI:3122; -.
DR   Proteomes; UP000009058; Chromosome 7.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
PE   1: Evidence at protein level;
KW   Disulfide bond; Reference proteome; Secreted; Signal; Virulence.
FT   SIGNAL          1..16
FT                   /evidence="ECO:0000255"
FT   CHAIN           17..54
FT                   /note="Secreted virulence factor MC69"
FT                   /id="PRO_5010834286"
FT   DISULFID        36..46
FT                   /evidence="ECO:0000269|PubMed:22589729"
FT   MUTAGEN         36
FT                   /note="C->A: Significantly reduces appressorial penetration
FT                   rate and blast symptoms on barley and rice."
FT                   /evidence="ECO:0000269|PubMed:22589729"
FT   MUTAGEN         46
FT                   /note="C->A: Significantly reduces appressorial penetration
FT                   rate and blast symptoms on barley and rice."
FT                   /evidence="ECO:0000269|PubMed:22589729"
SQ   SEQUENCE   54 AA;  5666 MW;  D8FA5892F4785881 CRC64;
     MKAAFVLALC ASLASAWVVI TPITKEMVVD RSPDDCFFGV VTPQGCGPLR GPAK