ID   MCA1_PLABA              Reviewed;         595 AA.
AC   A0A509APC7;
DT   07-OCT-2020, integrated into UniProtKB/Swiss-Prot.
DT   18-SEP-2019, sequence version 1.
DT   03-AUG-2022, entry version 10.
DE   RecName: Full=Metacaspase-1 {ECO:0000303|PubMed:17335919};
DE            EC=3.4.22.- {ECO:0000250|UniProtKB:Q8IDF3};
DE   AltName: Full=PfMC1 {ECO:0000303|PubMed:17335919};
DE   Contains:
DE     RecName: Full=Large subunit p20 {ECO:0000250|UniProtKB:Q08601};
DE   Contains:
DE     RecName: Full=Small subunit p10 {ECO:0000250|UniProtKB:Q08601};
DE   Flags: Precursor;
GN   Name=MCA1 {ECO:0000250|UniProtKB:Q8IDF3};
GN   ORFNames=PBANKA_1131400 {ECO:0000312|EMBL:VUC56735.1};
OS   Plasmodium berghei (strain Anka).
OC   Eukaryota; Sar; Alveolata; Apicomplexa; Aconoidasida; Haemosporida;
OC   Plasmodiidae; Plasmodium; Plasmodium (Vinckeia).
OX   NCBI_TaxID=5823 {ECO:0000312|Proteomes:UP000074855};
RN   [1] {ECO:0000312|Proteomes:UP000074855}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ANKA {ECO:0000312|Proteomes:UP000074855};
RX   PubMed=25359557; DOI=10.1186/s12915-014-0086-0;
RA   Otto T.D., Bohme U., Jackson A.P., Hunt M., Franke-Fayard B.,
RA   Hoeijmakers W.A., Religa A.A., Robertson L., Sanders M., Ogun S.A.,
RA   Cunningham D., Erhart A., Billker O., Khan S.M., Stunnenberg H.G.,
RA   Langhorne J., Holder A.A., Waters A.P., Newbold C.I., Pain A., Berriman M.,
RA   Janse C.J.;
RT   "A comprehensive evaluation of rodent malaria parasite genomes and gene
RT   expression.";
RL   BMC Biol. 12:86-86(2014).
RN   [2] {ECO:0000305}
RP   DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RX   PubMed=17335919; DOI=10.1016/j.molbiopara.2007.01.016;
RA   Le Chat L., Sinden R.E., Dessens J.T.;
RT   "The role of metacaspase 1 in Plasmodium berghei development and
RT   apoptosis.";
RL   Mol. Biochem. Parasitol. 153:41-47(2007).
CC   -!- FUNCTION: Cysteine protease that cleaves specifically after arginine or
CC       lysine residues. {ECO:0000250|UniProtKB:Q8IDF3}.
CC   -!- ACTIVITY REGULATION: Activated by Ca(2+).
CC       {ECO:0000250|UniProtKB:Q8IDF3}.
CC   -!- SUBUNIT: Monomer. {ECO:0000250|UniProtKB:Q08601}.
CC   -!- DEVELOPMENTAL STAGE: Expressed in female gametocytes and all downstream
CC       mosquito stages including the sporozoites inside the oocyst and the
CC       salivary gland, but not in asexual blood stages.
CC       {ECO:0000269|PubMed:17335919}.
CC   -!- DISRUPTION PHENOTYPE: No developmental defects in mice and mosquito
CC       hosts (PubMed:17335919). Not required for ookinete apoptosis
CC       (PubMed:17335919). {ECO:0000269|PubMed:17335919}.
CC   -!- SIMILARITY: Belongs to the peptidase C14B family. {ECO:0000305}.
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DR   EMBL; LK023126; VUC56735.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A509APC7; -.
DR   SMR; A0A509APC7; -.
DR   STRING; 5823.A0A509APC7; -.
DR   VEuPathDB; PlasmoDB:PBANKA_1131400; -.
DR   OMA; FMASQKF; -.
DR   Proteomes; UP000074855; Chromosome 11.
DR   GO; GO:0008234; F:cysteine-type peptidase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   InterPro; IPR035892; C2_domain_sf.
DR   SUPFAM; SSF49562; SSF49562; 1.
PE   2: Evidence at transcript level;
KW   Hydrolase; Protease; Reference proteome; Thiol protease; Zymogen.
FT   PROPEP          1..?
FT                   /evidence="ECO:0000305"
FT                   /id="PRO_0000451184"
FT   CHAIN           ?..480
FT                   /note="Large subunit p20"
FT                   /evidence="ECO:0000305"
FT                   /id="PRO_0000451186"
FT   CHAIN           481..595
FT                   /note="Small subunit p10"
FT                   /evidence="ECO:0000305"
FT                   /id="PRO_0000451187"
FT   CHAIN           ?..595
FT                   /note="Metacaspase-1"
FT                   /id="PRO_0000451185"
FT   ACT_SITE        411
FT                   /evidence="ECO:0000250|UniProtKB:Q08601"
FT   ACT_SITE        466
FT                   /evidence="ECO:0000250|UniProtKB:Q08601"
SQ   SEQUENCE   595 AA;  69604 MW;  51C761E49164C478 CRC64;
     MSLQMDKIYV KVHELKFVNN LERNSHYVKI YWDDKKYKSQ TKDGGCYIFN ETFLIPITNI
     YDQKDQIIYV EIWESNLLNK QCAYTFFTLN SIKIGQIIKE NITFIEVLKK CTLGLSINIV
     RNQKDILFFN IKELLPTFQD QEIINAVWKN EDEASIIKQL INLNTIDGIT NIGNYKNSQN
     YNETLQKPKE NISIYKSGEE IENSYIPSST PEYVSHYIYK GAGENSSNYI NKTKDTLFPT
     YLNNYAYNNN IKNVYDTPNG AHYSSNNNSG SNNAYSNFDH NINNSPKNNV PFSNSDNDKI
     FHYSRNYMPS PNSEKMLYFS CGNKKKALLI GIDYCGTQNE LKGSINDAII TNELLIKKYN
     FYDSSMNILK LIDNQTNPNY RPTKRNILSA LEWLVQDNNP GDIFFFFYSG HSYKKYDYTC
     IEKGGYNQTI VPCDFKTEGE IIDNDLHKYL IQPLKDGVKL VSFIDCPNSE GILNLGYKYK
     LKKEKWKETY NPFHVLADVT QFSYSKINEF PTEINLLEHV LITNNIEALT YHYMIQSIHS
     YINLYNKKKK KKIFLMSSQK FNIDRKFDFN HILKNSNSEL GQQKNIIKWK KNKKK