MCA1_PLAF7
ID MCA1_PLAF7 Reviewed; 613 AA.
AC Q8IDF3;
DT 07-OCT-2020, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 25-MAY-2022, entry version 99.
DE RecName: Full=Metacaspase-1 {ECO:0000303|PubMed:17492602};
DE EC=3.4.22.- {ECO:0000269|PubMed:21858231};
DE AltName: Full=PfMCA1 {ECO:0000303|PubMed:17492602};
DE Contains:
DE RecName: Full=Large subunit p20 {ECO:0000250|UniProtKB:Q08601};
DE Contains:
DE RecName: Full=Small subunit p10 {ECO:0000250|UniProtKB:Q08601};
DE Flags: Precursor;
GN Name=MCA1 {ECO:0000303|PubMed:17492602};
GN ORFNames=PF3D7_1354800 {ECO:0000312|EMBL:CAD52669.1};
OS Plasmodium falciparum (isolate 3D7).
OC Eukaryota; Sar; Alveolata; Apicomplexa; Aconoidasida; Haemosporida;
OC Plasmodiidae; Plasmodium; Plasmodium (Laverania).
OX NCBI_TaxID=36329 {ECO:0000312|Proteomes:UP000001450};
RN [1] {ECO:0000312|Proteomes:UP000001450}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=3D7 {ECO:0000312|Proteomes:UP000001450};
RX PubMed=12368864; DOI=10.1038/nature01097;
RA Gardner M.J., Hall N., Fung E., White O., Berriman M., Hyman R.W.,
RA Carlton J.M., Pain A., Nelson K.E., Bowman S., Paulsen I.T., James K.D.,
RA Eisen J.A., Rutherford K.M., Salzberg S.L., Craig A., Kyes S., Chan M.-S.,
RA Nene V., Shallom S.J., Suh B., Peterson J., Angiuoli S., Pertea M.,
RA Allen J., Selengut J., Haft D., Mather M.W., Vaidya A.B., Martin D.M.A.,
RA Fairlamb A.H., Fraunholz M.J., Roos D.S., Ralph S.A., McFadden G.I.,
RA Cummings L.M., Subramanian G.M., Mungall C., Venter J.C., Carucci D.J.,
RA Hoffman S.L., Newbold C., Davis R.W., Fraser C.M., Barrell B.G.;
RT "Genome sequence of the human malaria parasite Plasmodium falciparum.";
RL Nature 419:498-511(2002).
RN [2] {ECO:0000312|Proteomes:UP000001450}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=3D7 {ECO:0000312|Proteomes:UP000001450};
RX PubMed=12368867; DOI=10.1038/nature01095;
RA Hall N., Pain A., Berriman M., Churcher C.M., Harris B., Harris D.,
RA Mungall K.L., Bowman S., Atkin R., Baker S., Barron A., Brooks K.,
RA Buckee C.O., Burrows C., Cherevach I., Chillingworth C., Chillingworth T.,
RA Christodoulou Z., Clark L., Clark R., Corton C., Cronin A., Davies R.M.,
RA Davis P., Dear P., Dearden F., Doggett J., Feltwell T., Goble A.,
RA Goodhead I., Gwilliam R., Hamlin N., Hance Z., Harper D., Hauser H.,
RA Hornsby T., Holroyd S., Horrocks P., Humphray S., Jagels K., James K.D.,
RA Johnson D., Kerhornou A., Knights A., Konfortov B., Kyes S., Larke N.,
RA Lawson D., Lennard N., Line A., Maddison M., Mclean J., Mooney P.,
RA Moule S., Murphy L., Oliver K., Ormond D., Price C., Quail M.A.,
RA Rabbinowitsch E., Rajandream M.A., Rutter S., Rutherford K.M., Sanders M.,
RA Simmonds M., Seeger K., Sharp S., Smith R., Squares R., Squares S.,
RA Stevens K., Taylor K., Tivey A., Unwin L., Whitehead S., Woodward J.R.,
RA Sulston J.E., Craig A., Newbold C., Barrell B.G.;
RT "Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13.";
RL Nature 419:527-531(2002).
RN [3] {ECO:0000305}
RP PROTEOLYTIC CLEAVAGE.
RX PubMed=17492602; DOI=10.1086/518253;
RA Meslin B., Barnadas C., Boni V., Latour C., De Monbrison F., Kaiser K.,
RA Picot S.;
RT "Features of apoptosis in Plasmodium falciparum erythrocytic stage through
RT a putative role of PfMCA1 metacaspase-like protein.";
RL J. Infect. Dis. 195:1852-1859(2007).
RN [4] {ECO:0000305}
RP FUNCTION, CATALYTIC ACTIVITY, ACTIVITY REGULATION, AND PROTEOLYTIC
RP CLEAVAGE.
RX PubMed=21858231; DOI=10.1371/journal.pone.0023867;
RA Meslin B., Beavogui A.H., Fasel N., Picot S.;
RT "Plasmodium falciparum metacaspase PfMCA-1 triggers a z-VAD-fmk inhibitable
RT protease to promote cell death.";
RL PLoS ONE 6:e23867-e23867(2011).
CC -!- FUNCTION: Cysteine protease that cleaves specifically after arginine or
CC lysine residues (PubMed:21858231). May play a role in apoptosis
CC (PubMed:21858231). {ECO:0000269|PubMed:21858231}.
CC -!- ACTIVITY REGULATION: Activated by Ca(2+).
CC {ECO:0000269|PubMed:21858231}.
CC -!- SUBUNIT: Monomer. {ECO:0000250|UniProtKB:Q08601}.
CC -!- SIMILARITY: Belongs to the peptidase C14B family. {ECO:0000305}.
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DR EMBL; AL844509; CAD52669.1; -; Genomic_DNA.
DR RefSeq; XP_001350260.1; XM_001350224.1.
DR AlphaFoldDB; Q8IDF3; -.
DR SMR; Q8IDF3; -.
DR STRING; 5833.PF13_0289; -.
DR MEROPS; C14.041; -.
DR EnsemblProtists; CAD52669; CAD52669; PF3D7_1354800.
DR GeneID; 814253; -.
DR KEGG; pfa:PF3D7_1354800; -.
DR VEuPathDB; PlasmoDB:PF3D7_1354800; -.
DR HOGENOM; CLU_455980_0_0_1; -.
DR InParanoid; Q8IDF3; -.
DR OMA; FMASQKF; -.
DR PhylomeDB; Q8IDF3; -.
DR Proteomes; UP000001450; Chromosome 13.
DR GO; GO:0004198; F:calcium-dependent cysteine-type endopeptidase activity; IDA:UniProtKB.
DR GO; GO:0006915; P:apoptotic process; IDA:GeneDB.
DR GO; GO:0006508; P:proteolysis; IDA:UniProtKB.
DR Gene3D; 2.60.40.150; -; 1.
DR InterPro; IPR035892; C2_domain_sf.
DR InterPro; IPR029030; Caspase-like_dom_sf.
DR SUPFAM; SSF49562; SSF49562; 1.
DR SUPFAM; SSF52129; SSF52129; 1.
PE 1: Evidence at protein level;
KW Apoptosis; Hydrolase; Protease; Reference proteome; Thiol protease;
KW Zymogen.
FT PROPEP 1..?
FT /evidence="ECO:0000305"
FT /id="PRO_0000451178"
FT CHAIN ?..473
FT /note="Large subunit p20"
FT /evidence="ECO:0000305"
FT /id="PRO_0000451180"
FT CHAIN 474..613
FT /note="Small subunit p10"
FT /evidence="ECO:0000305"
FT /id="PRO_0000451181"
FT CHAIN ?..613
FT /note="Metacaspase-1"
FT /id="PRO_0000451179"
FT ACT_SITE 404
FT /evidence="ECO:0000250|UniProtKB:Q08601"
FT ACT_SITE 460
FT /evidence="ECO:0000250|UniProtKB:Q08601"
SQ SEQUENCE 613 AA; 71690 MW; FBD4753139732D43 CRC64;
MEKIYVKIYE LSGLEDKDNF SCYIKIYWQN KKYKSCILQK NPYKFNEIFL LPIDIKNNVK
DEKNNILSIE VWSSGILNNN KIAYTFFELD HIRRERISSE KINLIDVVKK CTLQISVHII
NNNQDILFCN IKDIFGNNKN DKEIHDAILK YGGNERHIIK ELRKEKEIGQ YNNIYFNDYV
NVLNTDPSQN YIYNDMPKIT PNNIYNNMNN DQTNHTYLKA PNSLYNNENT IYSSNVHYST
YMNNSPTYKN SNNMNHVTNM YASNDLHNSN HFKPHSNAYS TINYDNNNYI YPQNHTNIYN
RASPGSDQTL YFSPCNQKKA LLIGINYYGT KYELNGCTND TLRMKDLLVT KYKFYDSSNN
IVRLIDNEAN PNYRPTRRNI LSALMWLTRD NKPGDILFFL FSGHGSQEKD HNHIEKDGYN
ESILPSDFET EGVIIDDELH KYLIQPLNEG VKLIAVVDSC NSGSSIDLAY KYKLKSKKWK
EDKNPFHVIC DVTQFSGCKD KEVSYEVNTG QIAPGGSLVT AMVQILKNNM NTPSIITYEY
LLHNIHAHVK QHSNQTVTFM SSQKFNMNRL FDFEHIIKNK NNQLGQIINK YIEKNKSKNK
NKLKHELKNL FFF
