MCA2_ARATH
ID MCA2_ARATH Reviewed; 418 AA.
AC Q7XJE5; Q682S4; Q9SW15;
DT 20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2003, sequence version 1.
DT 03-AUG-2022, entry version 109.
DE RecName: Full=Metacaspase-2;
DE Short=AtMC2;
DE EC=3.4.22.-;
DE AltName: Full=Metacaspase 1c;
DE Short=AtMCP1c;
GN Name=AMC2; Synonyms=MCP1C; OrderedLocusNames=At4g25110;
GN ORFNames=F13M23.250;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), GENE FAMILY, AND NOMENCLATURE.
RX PubMed=15326173; DOI=10.1074/jbc.m406329200;
RA Vercammen D., van de Cotte B., De Jaeger G., Eeckhout D., Casteels P.,
RA Vandepoele K., Vandenberghe I., van Beeumen J., Inze D., van Breusegem F.;
RT "Type II metacaspases Atmc4 and Atmc9 of Arabidopsis thaliana cleave
RT substrates after arginine and lysine.";
RL J. Biol. Chem. 279:45329-45336(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RA Ikeda Y., Krishnamurthy N., Chua N.-H.;
RT "Characterization of metacaspases.";
RL Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RA Quinitio C., Chen H., Kim C.J., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP GENE FAMILY.
RX PubMed=20565583; DOI=10.1111/j.1364-3703.2004.00206.x;
RA Watanabe N., Lam E.;
RT "Recent advance in the study of caspase-like proteases and Bax inhibitor-1
RT in plants: their possible roles as regulator of programmed cell death.";
RL Mol. Plant Pathol. 5:65-70(2004).
RN [8]
RP FUNCTION, DISRUPTION PHENOTYPE, AND MUTAGENESIS OF CYS-135 AND CYS-256.
RX PubMed=21097903; DOI=10.1126/science.1194980;
RA Coll N.S., Vercammen D., Smidler A., Clover C., Van Breusegem F.,
RA Dangl J.L., Epple P.;
RT "Arabidopsis type I metacaspases control cell death.";
RL Science 330:1393-1397(2010).
CC -!- FUNCTION: Acts as a negative regulator of oxidative stress cell death
CC and hypersensitive cell death response mediated by immune response.
CC Acts via indirect or direct regulation of AMC1 at postranscriptional
CC level. {ECO:0000269|PubMed:21097903}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q7XJE5-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q7XJE5-2; Sequence=VSP_033690;
CC -!- DISRUPTION PHENOTYPE: No visible phenotype under normal growth
CC conditions, but enhancement of hypersensitive cell death response upon
CC infection with avirulent pathogen. {ECO:0000269|PubMed:21097903}.
CC -!- MISCELLANEOUS: [Isoform 2]: May be due to a competing acceptor splice
CC site. {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the peptidase C14B family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB36753.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAB79420.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AY219827; AAP44515.1; -; mRNA.
DR EMBL; AY322526; AAP84707.1; -; mRNA.
DR EMBL; AL035523; CAB36753.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL161562; CAB79420.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002687; AEE85012.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE85013.1; -; Genomic_DNA.
DR EMBL; BT026456; ABH04563.1; -; mRNA.
DR EMBL; AK175293; BAD43056.1; -; mRNA.
DR EMBL; AK221790; BAD93928.1; -; mRNA.
DR PIR; T05532; T05532.
DR RefSeq; NP_001031711.1; NM_001036634.1. [Q7XJE5-2]
DR RefSeq; NP_194241.3; NM_118643.5. [Q7XJE5-1]
DR AlphaFoldDB; Q7XJE5; -.
DR SMR; Q7XJE5; -.
DR IntAct; Q7XJE5; 1.
DR STRING; 3702.AT4G25110.1; -.
DR MEROPS; C14.A04; -.
DR PaxDb; Q7XJE5; -.
DR PRIDE; Q7XJE5; -.
DR ProteomicsDB; 238848; -. [Q7XJE5-1]
DR EnsemblPlants; AT4G25110.1; AT4G25110.1; AT4G25110. [Q7XJE5-1]
DR EnsemblPlants; AT4G25110.2; AT4G25110.2; AT4G25110. [Q7XJE5-2]
DR GeneID; 828614; -.
DR Gramene; AT4G25110.1; AT4G25110.1; AT4G25110. [Q7XJE5-1]
DR Gramene; AT4G25110.2; AT4G25110.2; AT4G25110. [Q7XJE5-2]
DR KEGG; ath:AT4G25110; -.
DR Araport; AT4G25110; -.
DR TAIR; locus:2117288; AT4G25110.
DR eggNOG; KOG1546; Eukaryota.
DR HOGENOM; CLU_029389_2_4_1; -.
DR InParanoid; Q7XJE5; -.
DR OMA; YLCRMER; -.
DR PhylomeDB; Q7XJE5; -.
DR PRO; PR:Q7XJE5; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q7XJE5; baseline and differential.
DR Genevisible; Q7XJE5; AT.
DR GO; GO:0004197; F:cysteine-type endopeptidase activity; IBA:GO_Central.
DR GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR GO; GO:0043069; P:negative regulation of programmed cell death; IGI:TAIR.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR InterPro; IPR033180; Metacaspase_type_1_plant.
DR InterPro; IPR005735; Znf_LSD1.
DR PANTHER; PTHR48104:SF6; PTHR48104:SF6; 1.
DR Pfam; PF06943; zf-LSD1; 1.
DR TIGRFAMs; TIGR01053; LSD1; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Hydrolase; Plant defense; Protease;
KW Reference proteome; Thiol protease.
FT CHAIN 1..418
FT /note="Metacaspase-2"
FT /id="PRO_0000334600"
FT REGION 68..113
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 385..406
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 200
FT /evidence="ECO:0000250"
FT ACT_SITE 256
FT /evidence="ECO:0000250"
FT VAR_SEQ 164
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|Ref.6"
FT /id="VSP_033690"
FT MUTAGEN 135
FT /note="C->A: No effect on the cell death inhibitory
FT function."
FT /evidence="ECO:0000269|PubMed:21097903"
FT MUTAGEN 256
FT /note="C->A: No effect on the cell death inhibitory
FT function."
FT /evidence="ECO:0000269|PubMed:21097903"
SQ SEQUENCE 418 AA; 45811 MW; 3195739BCB646B1D CRC64;
MLLLVDCSSC RTPLHLPPGA TRIRCAICHA FTLIAPEPRL QSHASASPFP FPNSSPAPST
FIYPPPTPSP YTHAPHAPSP FNHAPPDSYP FTHAPPASSP FNHAPPGPPP PVHGQKRAVI
VGVSYKNTKD ELKGCINDAN CMKFMLMKRF QFPESCILML TEEEADPMRW PTKNNITMAM
HWLVLSCKPG DSLVFHFSGH GNNQMDDNGD EVDGFDETLL PVDHRTSGVI VDDEINATIV
RPLPYGVKLH AIVDACHSGT VMDLPYLCRM DRLGNYEWED HRPKTGMWKG TSGGEVFSFT
GCDDDQTSAD TPQLSGSAWT GAMTYAFIQA IERGHGMTYG SLLNAMRSTV HEIFDKNKGR
ELVEVGGADF LSTLLGLLIL GASPPDEEEE VNQAPQKTQE PQLSANEAFA VYEKPFSL
