MCA3_PLAF7
ID MCA3_PLAF7 Reviewed; 2280 AA.
AC Q8ILT4;
DT 07-OCT-2020, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 25-MAY-2022, entry version 94.
DE RecName: Full=Metacaspase-3 {ECO:0000303|PubMed:31301397};
DE EC=3.4.22.- {ECO:0000269|PubMed:31301397};
DE AltName: Full=PfMCA3 {ECO:0000303|PubMed:31301397};
GN Name=MCA3 {ECO:0000303|PubMed:31301397};
GN ORFNames=PF3D7_1416200 {ECO:0000312|EMBL:CZT99873.1};
OS Plasmodium falciparum (isolate 3D7).
OC Eukaryota; Sar; Alveolata; Apicomplexa; Aconoidasida; Haemosporida;
OC Plasmodiidae; Plasmodium; Plasmodium (Laverania).
OX NCBI_TaxID=36329 {ECO:0000312|Proteomes:UP000001450};
RN [1] {ECO:0000312|Proteomes:UP000001450}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=3D7 {ECO:0000312|Proteomes:UP000001450};
RX PubMed=12368864; DOI=10.1038/nature01097;
RA Gardner M.J., Hall N., Fung E., White O., Berriman M., Hyman R.W.,
RA Carlton J.M., Pain A., Nelson K.E., Bowman S., Paulsen I.T., James K.D.,
RA Eisen J.A., Rutherford K.M., Salzberg S.L., Craig A., Kyes S., Chan M.-S.,
RA Nene V., Shallom S.J., Suh B., Peterson J., Angiuoli S., Pertea M.,
RA Allen J., Selengut J., Haft D., Mather M.W., Vaidya A.B., Martin D.M.A.,
RA Fairlamb A.H., Fraunholz M.J., Roos D.S., Ralph S.A., McFadden G.I.,
RA Cummings L.M., Subramanian G.M., Mungall C., Venter J.C., Carucci D.J.,
RA Hoffman S.L., Newbold C., Davis R.W., Fraser C.M., Barrell B.G.;
RT "Genome sequence of the human malaria parasite Plasmodium falciparum.";
RL Nature 419:498-511(2002).
RN [2] {ECO:0000305}
RP FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, AND
RP DEVELOPMENTAL STAGE.
RX PubMed=31301397; DOI=10.1016/j.ijbiomac.2019.07.067;
RA Kumar B., Verma S., Kashif M., Sharma R., Atul X., Dixit R., Singh A.P.,
RA Pande V., Saxena A.K., Abid M., Pandey K.C.;
RT "Metacaspase-3 of Plasmodium falciparum: An atypical trypsin-like serine
RT protease.";
RL Int. J. Biol. Macromol. 138:309-320(2019).
CC -!- FUNCTION: Protease that cleaves specifically after arginine or lysine
CC residues. {ECO:0000269|PubMed:31301397}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC pH dependence:
CC Optimum pH is 7.5. Has activity up to pH 9.5.
CC {ECO:0000269|PubMed:31301397};
CC Temperature dependence:
CC Optimum temperature is 25 degrees Celsius.
CC {ECO:0000269|PubMed:31301397};
CC -!- DEVELOPMENTAL STAGE: Expressed during all parasite blood stages (at
CC protein level). {ECO:0000269|PubMed:31301397}.
CC -!- SIMILARITY: Belongs to the peptidase C14B family. {ECO:0000305}.
CC -!- CAUTION: In contrast to other metacaspases of the peptidase C14B
CC family, the catalytic histidine and cysteine residues do not appear to
CC be conserved. Contains a serine residue at the position of the
CC canonical catalytic cysteine which may constitute the active site.
CC {ECO:0000305, ECO:0000305|PubMed:31301397}.
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DR EMBL; LN999946; CZT99873.1; -; Genomic_DNA.
DR RefSeq; XP_001348333.1; XM_001348297.1.
DR AlphaFoldDB; Q8ILT4; -.
DR STRING; 5833.PF14_0160; -.
DR PRIDE; Q8ILT4; -.
DR EnsemblProtists; CZT99873; CZT99873; PF3D7_1416200.
DR GeneID; 811741; -.
DR KEGG; pfa:PF3D7_1416200; -.
DR VEuPathDB; PlasmoDB:PF3D7_1416200; -.
DR HOGENOM; CLU_230367_0_0_1; -.
DR InParanoid; Q8ILT4; -.
DR OMA; DHKFIEL; -.
DR Proteomes; UP000001450; Chromosome 14.
DR GO; GO:0008236; F:serine-type peptidase activity; IDA:UniProtKB.
DR GO; GO:0006508; P:proteolysis; IDA:UniProtKB.
PE 1: Evidence at protein level;
KW Hydrolase; Protease; Reference proteome.
FT CHAIN 1..2280
FT /note="Metacaspase-3"
FT /id="PRO_0000451189"
FT REGION 56..83
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 202..284
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 791..819
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 931..954
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 994..1015
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1278..1306
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1469..1500
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT SITE 1865
FT /note="Important for catalytic activity"
FT /evidence="ECO:0000269|PubMed:31301397"
SQ SEQUENCE 2280 AA; 264925 MW; A242FC5E400B69CA CRC64;
MVYNNGSLRG RKKIEEKYIP MNRKSTSLKD PSRTKNNNYI KTLDTYINEE DSPTKILSKG
KKNKNENIKK RINEKDNDTD REDAASLNVD YNKNVMKKYN TRGTFIEKVS SSERNIEHNN
NIIRKLGSKD YSKARVQTMT TNIGNHNALK KINPKVPSIT SRTKSYTKVM NENKYNNNNS
NNSNNNNINS MYYSKKLNNK KYSTEHTQSI DNNNITSKRL NNKKTATDKA QSTSRYCISN
SVKKSYSTKG YNTDRSQTMS RYTLQNKKDS NTNTNRKGYS THRGQIIQDN SNINNQAEKK
KRNMSLKKKY IKESSNSTIT GSNEKKNSNN ILKKNSTCDL NYLNKEYNNN NNMHEHAENV
YNINDNEGCS SMQIINDEDV QKNVPEFYTI NLGSETKNMG ENYIFKNRDK KIKPNIYNER
NNMDLMEHKL NDDMSYNFAD HIKDIECYIN QLNESNKCNI YKECDPSKLI SPVYDNVNDN
ILENNQQLNE YEKHNDILTT SYLKYYSHTN NNNHYYNDED VHLDLGKTNE GFSNVVKNMD
NIPWNETNKK ESFSNIKKKI DHTDDRHIAY VDNSSMKHTM NDKDQYVKNV PYNNTMEEVL
LLNEKRGNIL GSLISKNHDA KRATSNNNNI NNNKYYYYDN KYSEHGGDSV SSNIGYPHGY
KNNNIYNYVN NIKDLSSPLY CNTKTKRAHK FHTDNIMSDQ LNKYEDNNKN DGKFFSGNIS
SKQKGKNNLT NYTTHSSEYR IKSIESNLTK DYRKSLTYND IPNFLDYNIN NTNVQNSDFG
DSHYINRNVS YKNSMNSNDD IQNSNNNNNN NNNNNMVNNP NEYLVYTNEK HYVRINNKLY
EKTRDNTYIE VDSNSNFDII NDINSSHNSP LDNIKDNTLY HNTNKKEYSD YVSSYSNINK
YEDINLPNNI HVSKDGKYLF KNYSVSQNVS DDNSVQDSFF SRTERSNSQN RYDKNGVNHI
EQNYYNNSNR RDDIYYYHNQ LKKLERLTQQ NIKSGKYQRN IPQNDDNDHN DDDSEIKEVK
KDKTKIDMNK HLHLNDKKIF NQVKRENTGV HMNASKTIKN MKEVTYRNIN NDTNYNNNNV
KINYLYSDEL GKNKNRDTPL KTKQHGDSLD NKTELKKKIY NDIINNDIIN NNIINNDIIK
SGAINNNIVN NNLINNYNAD NRNVSNSIFT QHINNINNNF YNSDDERINI IDNNKRMDMT
KDESDRFRNN YFVELEKRKK NLGKTNNTTP ISLSKNETIN NMMINKRNFF NNYISDKVRD
KILKREISCS VNTVCSSKTN NKTNSNYSSN NNNDNNNNNN NSNPFILDNS IRKSNIIITT
SNNAKLINNT IISKGINNNI LNINSNKNKS LNSAKINALG LLSSRNDLNY IMNNNRTSNN
HINQHLLYNN RNYSVIKIPN VHKEKRSSKI PHNINIKSNL NLEPNIKTYT NIKTPINIKA
YTNIKAPTNI KAPINIKAST NIKAPTNVKA PTNVKAPTNV KAPTNVNAPT NVNAPTNANA
PTYVNTPTNI KRQEFSNVTL NYKGISKFDI NEDKNTTIIP PLHRQSNSNF PFKNTKLNLN
SDMYTEKELI KCKSINNSDI YNYLRNTYNK TPSKFYRTVS YRNMSLKKNK SMLENRKRKT
EKKEENLMST FNYTSEVDSK YIVKKAVVVG CNYVSEERSR LYGSVNDAYV FCRALVKYFD
FLPENILLLT DSLPSNAYIY EDFDINRKKY INVDEEENIK NNEPLKKKNI FNLFNTNALY
TTLKKTNEEE LNCNSCKDVE IKNVDISSEK MNFNLWPTRV NILKAVNWLV RDSIPFGSYV
FYFAGKSVQV DNMSGWEGEG YDEAFLCSDP FNKISEHNVI TAVQLKDLLL SINESAQMTI
ILDCSGGQTI LDPAGTENSL SYIKGCKQKG IWPITNPTNK VHKAIYDITI LNNTSMKKYF
CRSRYSKLIE VESTSAMIDP LLQSISSLPV APKAYCLCAA TWEQISIEGL FPIIEFARVS
QLKKPESYKT GEGHYQNMNK KNIRAEKSNR NGPNGLINKN NNLSKKTNYE KNFNFTLNMM
KMLFSNTNNN ENKLDEKEKI NRLGFNENDS DYIDDNYNSD DNNNNNYYYN YDGEKGFKNF
QENLNNVGQK DVIKNKKFKD NYILVSHGVF TYCLIEAIIE FKEKELKYNI LEKKNEQFIP
MTLKNLINVI QQKMQNIKYN KLKKINQKPE FTIHPGANAT NNNYFVHYSK NIHFQNYKCN
FINADLSPFL NVNKAWEEIN RTTLRNRKSL SLSSTLINTA SSKYFTQKNE QFKNSYSLKY
