ID   MCBC_ECOLX              Reviewed;         272 AA.
AC   P23185;
DT   01-NOV-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1991, sequence version 1.
DT   03-AUG-2022, entry version 79.
DE   RecName: Full=Microcin B17-processing protein McbC;
GN   Name=mcbC;
OS   Escherichia coli.
OG   Plasmid IncFII pMccB17.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2644225; DOI=10.1128/jb.171.2.1126-1135.1989;
RA   Genilloud O., Moreno F., Kolter R.;
RT   "DNA sequence, products, and transcriptional pattern of the genes involved
RT   in production of the DNA replication inhibitor microcin B17.";
RL   J. Bacteriol. 171:1126-1135(1989).
CC   -!- FUNCTION: Necessary to process the inactive microcin B17 (McbA)
CC       precursor into the active peptide.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- SIMILARITY: To R.leguminosarum TfxB which is involved in the processing
CC       of trifolitoxin. {ECO:0000305}.
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DR   EMBL; M24253; AAA72743.1; -; Genomic_DNA.
DR   PIR; C32058; C32058.
DR   RefSeq; WP_001528601.1; NZ_WSWV01000078.1.
DR   PDB; 6GOS; X-ray; 2.10 A; C=1-272.
DR   PDB; 6GRG; X-ray; 2.35 A; C=1-272.
DR   PDB; 6GRH; X-ray; 1.85 A; C=1-272.
DR   PDB; 6GRI; X-ray; 2.70 A; C=1-272.
DR   PDBsum; 6GOS; -.
DR   PDBsum; 6GRG; -.
DR   PDBsum; 6GRH; -.
DR   PDBsum; 6GRI; -.
DR   AlphaFoldDB; P23185; -.
DR   SMR; P23185; -.
DR   BioCyc; MetaCyc:MON-21088; -.
DR   BRENDA; 1.3.3.16; 2026.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016491; F:oxidoreductase activity; IEA:InterPro.
DR   GO; GO:0017000; P:antibiotic biosynthetic process; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.109.10; -; 1.
DR   InterPro; IPR029479; Nitroreductase.
DR   InterPro; IPR000415; Nitroreductase-like.
DR   InterPro; IPR020051; SagB-type_dehydrogenase.
DR   Pfam; PF00881; Nitroreductase; 1.
DR   SUPFAM; SSF55469; SSF55469; 1.
DR   TIGRFAMs; TIGR03605; antibiot_sagB; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Antibiotic biosynthesis; Cytoplasm; Plasmid.
FT   CHAIN           1..272
FT                   /note="Microcin B17-processing protein McbC"
FT                   /id="PRO_0000068572"
FT   HELIX           8..12
FT                   /evidence="ECO:0007829|PDB:6GRH"
FT   HELIX           17..27
FT                   /evidence="ECO:0007829|PDB:6GRH"
FT   HELIX           28..30
FT                   /evidence="ECO:0007829|PDB:6GRH"
FT   HELIX           36..38
FT                   /evidence="ECO:0007829|PDB:6GRH"
FT   TURN            39..41
FT                   /evidence="ECO:0007829|PDB:6GRH"
FT   HELIX           49..55
FT                   /evidence="ECO:0007829|PDB:6GRH"
FT   HELIX           74..76
FT                   /evidence="ECO:0007829|PDB:6GRH"
FT   STRAND          88..90
FT                   /evidence="ECO:0007829|PDB:6GRH"
FT   HELIX           97..108
FT                   /evidence="ECO:0007829|PDB:6GRH"
FT   HELIX           123..125
FT                   /evidence="ECO:0007829|PDB:6GRH"
FT   STRAND          129..135
FT                   /evidence="ECO:0007829|PDB:6GRH"
FT   STRAND          149..153
FT                   /evidence="ECO:0007829|PDB:6GRH"
FT   TURN            154..157
FT                   /evidence="ECO:0007829|PDB:6GRH"
FT   STRAND          158..163
FT                   /evidence="ECO:0007829|PDB:6GRH"
FT   HELIX           167..175
FT                   /evidence="ECO:0007829|PDB:6GRH"
FT   HELIX           176..178
FT                   /evidence="ECO:0007829|PDB:6GRH"
FT   HELIX           179..182
FT                   /evidence="ECO:0007829|PDB:6GRH"
FT   STRAND          186..194
FT                   /evidence="ECO:0007829|PDB:6GRH"
FT   HELIX           195..199
FT                   /evidence="ECO:0007829|PDB:6GRH"
FT   TURN            200..203
FT                   /evidence="ECO:0007829|PDB:6GRH"
FT   HELIX           204..227
FT                   /evidence="ECO:0007829|PDB:6GRH"
FT   STRAND          231..235
FT                   /evidence="ECO:0007829|PDB:6GRH"
FT   HELIX           240..246
FT                   /evidence="ECO:0007829|PDB:6GRH"
FT   TURN            251..253
FT                   /evidence="ECO:0007829|PDB:6GRH"
FT   STRAND          254..264
FT                   /evidence="ECO:0007829|PDB:6GRH"
SQ   SEQUENCE   272 AA;  30753 MW;  956E1744338C051D CRC64;
     MSKHELSLVE VTHYTDPEVL AIVKDFHVRG NFASLPEFAE RTFVSAVPLA HLEKFENKEV
     LFRPGFSSVI NISSSHNFSR ERLPSGINFC DKNKLSIRTI EKLLVNAFSS PDPGSVRRPY
     PSGGALYPIE VFLCRLSENT ENWQAGTNVY HYLPLSQALE PVATCNTQSL YRSLSGGDSE
     RLGKPHFALV YCIIFEKALF KYRYRGYRMA LMETGSMYQN AVLVADQIGL KNRVWAGYTD
     SYVAKTMNLD QRTVAPLIVQ FFGDVNDDKC LQ