ID   MCBD_ECOLX              Reviewed;         396 AA.
AC   P23186;
DT   01-NOV-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1991, sequence version 1.
DT   03-AUG-2022, entry version 55.
DE   RecName: Full=Microcin B17-processing protein McbD;
GN   Name=mcbD;
OS   Escherichia coli.
OG   Plasmid IncFII pMccB17.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2644225; DOI=10.1128/jb.171.2.1126-1135.1989;
RA   Genilloud O., Moreno F., Kolter R.;
RT   "DNA sequence, products, and transcriptional pattern of the genes involved
RT   in production of the DNA replication inhibitor microcin B17.";
RL   J. Bacteriol. 171:1126-1135(1989).
CC   -!- FUNCTION: Necessary to process the inactive microcin B17 (McbA)
CC       precursor into the active peptide.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
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DR   EMBL; M24253; AAA72744.1; -; Genomic_DNA.
DR   PIR; D32058; D32058.
DR   PDB; 6GOS; X-ray; 2.10 A; D=1-396.
DR   PDB; 6GRG; X-ray; 2.35 A; D=1-396.
DR   PDB; 6GRH; X-ray; 1.85 A; D=1-396.
DR   PDB; 6GRI; X-ray; 2.70 A; D=1-396.
DR   PDBsum; 6GOS; -.
DR   PDBsum; 6GRG; -.
DR   PDBsum; 6GRH; -.
DR   PDBsum; 6GRI; -.
DR   AlphaFoldDB; P23186; -.
DR   SMR; P23186; -.
DR   BioCyc; MetaCyc:MON-21087; -.
DR   BRENDA; 1.3.3.16; 2026.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0017000; P:antibiotic biosynthetic process; IEA:UniProtKB-KW.
DR   InterPro; IPR003776; YcaO-like_dom.
DR   Pfam; PF02624; YcaO; 1.
DR   PROSITE; PS51664; YCAO; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Antibiotic biosynthesis; Cytoplasm; Plasmid.
FT   CHAIN           1..396
FT                   /note="Microcin B17-processing protein McbD"
FT                   /id="PRO_0000068573"
FT   DOMAIN          41..396
FT                   /note="YcaO"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00999"
FT   STRAND          10..12
FT                   /evidence="ECO:0007829|PDB:6GRH"
FT   STRAND          14..18
FT                   /evidence="ECO:0007829|PDB:6GRH"
FT   STRAND          28..33
FT                   /evidence="ECO:0007829|PDB:6GRH"
FT   TURN            35..37
FT                   /evidence="ECO:0007829|PDB:6GRH"
FT   STRAND          41..46
FT                   /evidence="ECO:0007829|PDB:6GRH"
FT   HELIX           47..66
FT                   /evidence="ECO:0007829|PDB:6GRH"
FT   HELIX           76..79
FT                   /evidence="ECO:0007829|PDB:6GRH"
FT   HELIX           82..95
FT                   /evidence="ECO:0007829|PDB:6GRH"
FT   STRAND          96..98
FT                   /evidence="ECO:0007829|PDB:6GRI"
FT   HELIX           100..105
FT                   /evidence="ECO:0007829|PDB:6GRH"
FT   STRAND          108..115
FT                   /evidence="ECO:0007829|PDB:6GRH"
FT   TURN            116..118
FT                   /evidence="ECO:0007829|PDB:6GRH"
FT   STRAND          121..125
FT                   /evidence="ECO:0007829|PDB:6GRH"
FT   HELIX           126..128
FT                   /evidence="ECO:0007829|PDB:6GRH"
FT   STRAND          132..134
FT                   /evidence="ECO:0007829|PDB:6GRH"
FT   HELIX           137..142
FT                   /evidence="ECO:0007829|PDB:6GRH"
FT   STRAND          151..156
FT                   /evidence="ECO:0007829|PDB:6GRH"
FT   HELIX           157..180
FT                   /evidence="ECO:0007829|PDB:6GRH"
FT   STRAND          182..187
FT                   /evidence="ECO:0007829|PDB:6GRH"
FT   HELIX           196..208
FT                   /evidence="ECO:0007829|PDB:6GRH"
FT   STRAND          209..215
FT                   /evidence="ECO:0007829|PDB:6GRH"
FT   STRAND          222..231
FT                   /evidence="ECO:0007829|PDB:6GRH"
FT   STRAND          240..250
FT                   /evidence="ECO:0007829|PDB:6GRH"
FT   HELIX           251..275
FT                   /evidence="ECO:0007829|PDB:6GRH"
FT   HELIX           279..281
FT                   /evidence="ECO:0007829|PDB:6GRH"
FT   HELIX           285..292
FT                   /evidence="ECO:0007829|PDB:6GRH"
FT   HELIX           296..304
FT                   /evidence="ECO:0007829|PDB:6GRH"
FT   STRAND          307..309
FT                   /evidence="ECO:0007829|PDB:6GRH"
FT   STRAND          314..316
FT                   /evidence="ECO:0007829|PDB:6GRH"
FT   TURN            321..324
FT                   /evidence="ECO:0007829|PDB:6GRH"
FT   HELIX           327..334
FT                   /evidence="ECO:0007829|PDB:6GRH"
FT   STRAND          339..347
FT                   /evidence="ECO:0007829|PDB:6GRH"
FT   STRAND          350..358
FT                   /evidence="ECO:0007829|PDB:6GRH"
FT   STRAND          364..366
FT                   /evidence="ECO:0007829|PDB:6GRH"
FT   STRAND          375..378
FT                   /evidence="ECO:0007829|PDB:6GRH"
FT   HELIX           386..390
FT                   /evidence="ECO:0007829|PDB:6GRH"
SQ   SEQUENCE   396 AA;  44865 MW;  110BF4FE1B5B3C4D CRC64;
     MINVYSNLMS AWPATMAMSP KLNRNMPTFS QIWDYERITP ASAAGETLKS IQGAIGEYFE
     RRHFFNEIVT GGQKTLYEMM PPSAAKAFTE AFFQISSLTR DEIITHKFKT VRAFNLFSLE
     QQEIPAVIIA LDNITAADDL KFYPDRDTCG CSFHGSLNDA IEGSLCEFME TQSLLLYWLQ
     GKANTEISSE IVTGINHIDE ILLALRSEGD IRIFDITLPG APGHAVLTLY GTKNKISRIK
     YSTGLSYANS LKKALCKSVV ELWQSYICLH NFLIGGYTDD DIIDSYQRHF MSCNKYESFT
     DLCENTVLLS DDVKLTLEEN ITSDTNLLNY LQQISDNIFV YYARERVSNS LVWYTKIVSP
     DFFLHMNNSG AININNKIYH TGDGIKVRES KMVPFP