ID   MCBE_ECOLX              Reviewed;         241 AA.
AC   P05528;
DT   01-NOV-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1988, sequence version 1.
DT   03-AUG-2022, entry version 87.
DE   RecName: Full=Protein McbE;
GN   Name=mcbE;
OS   Escherichia coli.
OG   Plasmid IncFII pMccB17.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3049078; DOI=10.1002/j.1460-2075.1988.tb03018.x;
RA   Garrido M.D.C., Herrero M., Kolter R., Moreno F.;
RT   "The export of the DNA replication inhibitor microcin B17 provides immunity
RT   for the host cell.";
RL   EMBO J. 7:1853-1862(1988).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-93.
RX   PubMed=2644225; DOI=10.1128/jb.171.2.1126-1135.1989;
RA   Genilloud O., Moreno F., Kolter R.;
RT   "DNA sequence, products, and transcriptional pattern of the genes involved
RT   in production of the DNA replication inhibitor microcin B17.";
RL   J. Bacteriol. 171:1126-1135(1989).
CC   -!- FUNCTION: Together with two further proteins McbF and McbG this protein
CC       causes immunity to the peptide antibiotic microcin B17 (MccB17), which
CC       inhibits DNA replication in enterobacteriaceae. Immunity is determined
CC       by two different mechanisms. McbE is involved in the production of
CC       extracellular MccB17 and, in a complex with McbF it also serves as
CC       'pump' for the export of active MccB17 from the cytoplasm to the
CC       periplasmic space.
CC   -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; X07875; CAA30722.1; -; Genomic_DNA.
DR   EMBL; M24253; AAA72745.1; -; Genomic_DNA.
DR   PIR; S00836; S00836.
DR   RefSeq; WP_000258040.1; NZ_WSWV01000078.1.
DR   AlphaFoldDB; P05528; -.
DR   TCDB; 3.A.1.116.1; the atp-binding cassette (abc) superfamily.
DR   BioCyc; MetaCyc:MON-21089; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
PE   4: Predicted;
KW   Antibiotic resistance; Cell membrane; Membrane; Plasmid; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..241
FT                   /note="Protein McbE"
FT                   /id="PRO_0000068574"
FT   TRANSMEM        17..35
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        54..72
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        105..123
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        131..149
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        163..181
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        212..230
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   241 AA;  27801 MW;  0AEAB9FD77B1BCD2 CRC64;
     MVTLKMAIIF LMEQIRTPFS LIWTIMSPTV LFFFLHFNEI ELHYGDTAWL GKQISWFVGY
     ISFSVVLFNY CLYLVGRRES GFIATFVHNM DGRLLFIRSQ LIASLIMSIL YVFFFILVVL
     TGFQASPDYQ IVMIILKSIY INAFMMVSLT FMASFRVTFQ TASTIYSVLI TVCMVSGIVS
     LKYNEGIVYW INQVNPIAIY STILQSDQEL SLMTIFFYSI MLIISIISAL TFKTEPVWSS
     Q