MCBR_ECOLI
ID MCBR_ECOLI Reviewed; 221 AA.
AC P76114; P77347;
DT 15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT 15-JUL-1999, sequence version 2.
DT 03-AUG-2022, entry version 151.
DE RecName: Full=HTH-type transcriptional regulator McbR;
GN Name=mcbR; Synonyms=yncC; OrderedLocusNames=b1450, JW1445;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=9097039; DOI=10.1093/dnares/3.6.363;
RA Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T.,
RA Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K.,
RA Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S.,
RA Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G.,
RA Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y.,
RA Wada C., Yamamoto Y., Horiuchi T.;
RT "A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT the 28.0-40.1 min region on the linkage map.";
RL DNA Res. 3:363-377(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP FUNCTION IN BIOFILM FORMATION, INDUCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=K12 / BW25113;
RX PubMed=18309357; DOI=10.1038/ismej.2008.24;
RA Zhang X.-S., Garcia-Contreras R., Wood T.K.;
RT "Escherichia coli transcription factor YncC (McbR) regulates colanic acid
RT and biofilm formation by repressing expression of periplasmic protein YbiM
RT (McbA).";
RL ISME J. 2:615-631(2008).
CC -!- FUNCTION: Important for biofilm formation. Represses expression of McbA
CC by binding to its promoter region, which prevents colanic acid
CC overproduction and mucoidy. {ECO:0000269|PubMed:18309357}.
CC -!- INTERACTION:
CC P76114; P60438: rplC; NbExp=2; IntAct=EBI-560824, EBI-542200;
CC -!- INDUCTION: Induced by LsrR, LsrK, MqsR and TqsA. Repressed by LuxS and
CC YgiV. {ECO:0000269|PubMed:18309357}.
CC -!- DISRUPTION PHENOTYPE: Disruption of this gene induces 30 genes and
CC represses 10 genes more than fourfold. It decreases biofilm formation,
CC increases extracellular indole, leads to colanic acid overproduction
CC and elicits mucoidy. {ECO:0000269|PubMed:18309357}.
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DR EMBL; U00096; AAC74532.2; -; Genomic_DNA.
DR EMBL; AP009048; BAA15082.1; -; Genomic_DNA.
DR PIR; E64897; E64897.
DR RefSeq; NP_415967.2; NC_000913.3.
DR RefSeq; WP_001300742.1; NZ_STEB01000043.1.
DR AlphaFoldDB; P76114; -.
DR SMR; P76114; -.
DR BioGRID; 4260196; 3.
DR BioGRID; 850375; 2.
DR DIP; DIP-12749N; -.
DR IntAct; P76114; 11.
DR STRING; 511145.b1450; -.
DR jPOST; P76114; -.
DR PaxDb; P76114; -.
DR PRIDE; P76114; -.
DR EnsemblBacteria; AAC74532; AAC74532; b1450.
DR EnsemblBacteria; BAA15082; BAA15082; BAA15082.
DR GeneID; 946014; -.
DR KEGG; ecj:JW1445; -.
DR KEGG; eco:b1450; -.
DR PATRIC; fig|1411691.4.peg.818; -.
DR EchoBASE; EB3535; -.
DR eggNOG; COG1802; Bacteria.
DR HOGENOM; CLU_017584_5_4_6; -.
DR InParanoid; P76114; -.
DR OMA; SLTMQVE; -.
DR PhylomeDB; P76114; -.
DR BioCyc; EcoCyc:G6761-MON; -.
DR PRO; PR:P76114; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:EcoCyc.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IDA:EcoCyc.
DR Gene3D; 1.10.10.10; -; 1.
DR Gene3D; 1.20.120.530; -; 1.
DR InterPro; IPR011711; GntR_C.
DR InterPro; IPR008920; TF_FadR/GntR_C.
DR InterPro; IPR000524; Tscrpt_reg_HTH_GntR.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR Pfam; PF07729; FCD; 1.
DR Pfam; PF00392; GntR; 1.
DR SMART; SM00895; FCD; 1.
DR SMART; SM00345; HTH_GNTR; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
DR SUPFAM; SSF48008; SSF48008; 1.
DR PROSITE; PS50949; HTH_GNTR; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Reference proteome; Repressor; Transcription;
KW Transcription regulation.
FT CHAIN 1..221
FT /note="HTH-type transcriptional regulator McbR"
FT /id="PRO_0000050686"
FT DOMAIN 10..77
FT /note="HTH gntR-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00307"
FT DNA_BIND 37..56
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00307"
SQ SEQUENCE 221 AA; 25151 MW; 7A589BCFAE20D75C CRC64;
MPGTGKMKHV SLTLQVENDL KHQLSIGALK PGARLITKNL AEQLGMSITP VREALLRLVS
VNALSVAPAQ AFTVPEVGKR QLDEINRIRY ELELMAVALA VENLTPQDLA ELQELLEKLQ
QAQEKGDMEQ IINVNRLFRL AIYHRSNMPI LCEMIEQLWV RMGPGLHYLY EAINPAELRE
HIENYHLLLA ALKAKDKEGC RHCLAEIMQQ NIAILYQQYN R
