ID   MCE3R_MYCTU             Reviewed;         406 AA.
AC   P95251; F2GGQ2; I6Y7Z5; Q7D7P3;
DT   30-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1998, sequence version 2.
DT   03-AUG-2022, entry version 154.
DE   RecName: Full=Transcriptional repressor Mce3R {ECO:0000303|PubMed:12368433};
DE   AltName: Full=TetR family transcriptional regulator {ECO:0000303|PubMed:12368433};
GN   Name=mce3R {ECO:0000303|PubMed:12368433}; OrderedLocusNames=Rv1963c;
GN   ORFNames=LH57_10685 {ECO:0000312|EMBL:AIR14708.1};
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 27294 / TMC 102 / H37Rv;
RA   Hazbon M.H., Riojas M.A., Damon A.M., Alalade R.O., Cantwell B.J.,
RA   Monaco A., King S., Sohrabi A.;
RT   "Phylogenetic analysis of Mycobacterial species using whole genome
RT   sequences.";
RL   Submitted (SEP-2014) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   FUNCTION.
RX   PubMed=12368433; DOI=10.1099/00221287-148-10-2997;
RA   Santangelo M.P., Goldstein J., Alito A., Gioffre A., Caimi K., Zabal O.,
RA   Zumarraga M., Romano M.I., Cataldi A.A., Bigi F.;
RT   "Negative transcriptional regulation of the mce3 operon in Mycobacterium
RT   tuberculosis.";
RL   Microbiology 148:2997-3006(2002).
RN   [4]
RP   FUNCTION, DISRUPTION PHENOTYPE, AND INDUCTION.
RC   STRAIN=H37Rv;
RX   PubMed=18304349; DOI=10.1186/1471-2180-8-38;
RA   Santangelo M.P., Blanco F.C., Bianco M.V., Klepp L.I., Zabal O.,
RA   Cataldi A.A., Bigi F.;
RT   "Study of the role of Mce3R on the transcription of mce genes of
RT   Mycobacterium tuberculosis.";
RL   BMC Microbiol. 8:38-38(2008).
RN   [5]
RP   FUNCTION.
RX   PubMed=19389781; DOI=10.1099/mic.0.027086-0;
RA   de la Paz Santangelo M., Klepp L., Nunez-Garcia J., Blanco F.C., Soria M.,
RA   Garcia-Pelayo M.C., Bianco M.V., Cataldi A.A., Golby P., Jackson M.,
RA   Gordon S.V., Bigi F.;
RT   "Mce3R, a TetR-type transcriptional repressor, controls the expression of a
RT   regulon involved in lipid metabolism in Mycobacterium tuberculosis.";
RL   Microbiology 155:2245-2255(2009).
RN   [6]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA   Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA   Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA   Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA   Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT   "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT   mass spectrometry.";
RL   Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
CC   -!- FUNCTION: Mce3R represses the transcription of mce3 operon and down-
CC       regulates its own expression, but does not affect the transcription of
CC       mce1, mce2 and mce4 operons. {ECO:0000269|PubMed:12368433,
CC       ECO:0000269|PubMed:18304349, ECO:0000269|PubMed:19389781}.
CC   -!- INDUCTION: Negatively autoregulated. {ECO:0000269|PubMed:18304349}.
CC   -!- DISRUPTION PHENOTYPE: Cells lacking this gene result in a significant
CC       increase in the expression of mce3 either in vitro or in a murine cell
CC       macrophages line. {ECO:0000269|PubMed:18304349}.
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DR   EMBL; CP009480; AIR14708.1; -; Genomic_DNA.
DR   EMBL; AL123456; CCP44732.1; -; Genomic_DNA.
DR   RefSeq; NP_216479.1; NC_000962.3.
DR   RefSeq; WP_003409919.1; NZ_NVQJ01000048.1.
DR   AlphaFoldDB; P95251; -.
DR   SMR; P95251; -.
DR   STRING; 83332.Rv1963c; -.
DR   PaxDb; P95251; -.
DR   PRIDE; P95251; -.
DR   GeneID; 885950; -.
DR   KEGG; mtu:Rv1963c; -.
DR   PATRIC; fig|83332.111.peg.2184; -.
DR   TubercuList; Rv1963c; -.
DR   eggNOG; COG1309; Bacteria.
DR   HOGENOM; CLU_055812_0_0_11; -.
DR   OMA; EARYLND; -.
DR   PhylomeDB; P95251; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IDA:MTBBASE.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IMP:MTBBASE.
DR   GO; GO:0000976; F:transcription cis-regulatory region binding; IBA:GO_Central.
DR   GO; GO:0016042; P:lipid catabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IMP:MTBBASE.
DR   GO; GO:0019216; P:regulation of lipid metabolic process; IDA:MTBBASE.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IBA:GO_Central.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR001647; HTH_TetR.
DR   Pfam; PF00440; TetR_N; 2.
DR   PRINTS; PR00455; HTHTETR.
DR   SUPFAM; SSF46689; SSF46689; 2.
DR   PROSITE; PS50977; HTH_TETR_2; 2.
PE   1: Evidence at protein level;
KW   DNA-binding; Lipid degradation; Lipid metabolism; Reference proteome;
KW   Repeat; Repressor; Transcription; Transcription regulation.
FT   CHAIN           1..406
FT                   /note="Transcriptional repressor Mce3R"
FT                   /id="PRO_0000438536"
FT   DOMAIN          13..73
FT                   /note="HTH tetR-type 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00335"
FT   DOMAIN          217..277
FT                   /note="HTH tetR-type 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00335"
FT   DNA_BIND        36..55
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00335"
FT   DNA_BIND        240..259
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00335"
SQ   SEQUENCE   406 AA;  44342 MW;  A268EDF92AD8E61B CRC64;
     MASVAQPVRR RPKDRKKQIL DQAVGLFIER GFHSVKLEDI AEAAGVTARA LYRHYDNKQA
     LLAEAIRTGQ DQYQSARRLT EGETEPTPRP LNADLEDLIA AAVASRALTV LWQREARYLN
     EDDRTAVRRR INAIVAGMRD SVLLEVPDLS PQHSELRAWA VSSTLTSLGR HSLSLPGEEL
     KKLLYQACMA AARTPPVCEL PPLPAGDAAR DEADVLFSRY ETLLAAGARL FRAQGYPAVN
     TSEIGKGAGI AGPGLYRSFS SKQAILDALI RRLDEWRCLE CIRALRANQQ AAQRLRGLVQ
     GHVRISLDAP DLVAVSVTEL SHASVEVRDG YLRNQGDREA VWIDLIGKLV PATSVAQGRL
     LVAAAISFIE DVARTWHLTR YAGVADEISG LALAILTSGA GNLLRA