MCEE_DICDI
ID MCEE_DICDI Reviewed; 166 AA.
AC Q553V2; Q86I72;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 03-AUG-2022, entry version 88.
DE RecName: Full=Methylmalonyl-CoA epimerase, mitochondrial {ECO:0000250|UniProtKB:Q96PE7, ECO:0000305};
DE EC=5.1.99.1;
DE AltName: Full=DL-methylmalonyl-CoA racemase;
DE Flags: Precursor;
GN Name=mcee; ORFNames=DDB_G0275181;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=12097910; DOI=10.1038/nature00847;
RA Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T.,
RA Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R.,
RA Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A.,
RA Noegel A.A.;
RT "Sequence and analysis of chromosome 2 of Dictyostelium discoideum.";
RL Nature 418:79-85(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
CC -!- FUNCTION: Methylmalonyl-CoA epimerase involved in propionyl-CoA
CC metabolism. {ECO:0000250|UniProtKB:Q96PE7}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(R)-methylmalonyl-CoA = (S)-methylmalonyl-CoA;
CC Xref=Rhea:RHEA:20553, ChEBI:CHEBI:57326, ChEBI:CHEBI:57327;
CC EC=5.1.99.1; Evidence={ECO:0000250|UniProtKB:Q96PE7};
CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:20555;
CC Evidence={ECO:0000250|UniProtKB:Q96PE7};
CC -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the methylmalonyl-CoA epimerase family.
CC {ECO:0000305}.
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DR EMBL; AAFI02000013; EAL69871.1; -; Genomic_DNA.
DR RefSeq; XP_643859.1; XM_638767.1.
DR AlphaFoldDB; Q553V2; -.
DR SMR; Q553V2; -.
DR STRING; 44689.DDB0231097; -.
DR PaxDb; Q553V2; -.
DR EnsemblProtists; EAL69871; EAL69871; DDB_G0275181.
DR GeneID; 8619910; -.
DR KEGG; ddi:DDB_G0275181; -.
DR dictyBase; DDB_G0275181; mcee.
DR eggNOG; KOG2944; Eukaryota.
DR HOGENOM; CLU_046006_5_0_1; -.
DR InParanoid; Q553V2; -.
DR OMA; IHHICYE; -.
DR PhylomeDB; Q553V2; -.
DR Reactome; R-DDI-71032; Propionyl-CoA catabolism.
DR PRO; PR:Q553V2; -.
DR Proteomes; UP000002195; Chromosome 2.
DR GO; GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0004493; F:methylmalonyl-CoA epimerase activity; ISS:dictyBase.
DR GO; GO:0046491; P:L-methylmalonyl-CoA metabolic process; ISS:dictyBase.
DR CDD; cd07249; MMCE; 1.
DR Gene3D; 3.10.180.10; -; 1.
DR InterPro; IPR029068; Glyas_Bleomycin-R_OHBP_Dase.
DR InterPro; IPR017515; MeMalonyl-CoA_epimerase.
DR InterPro; IPR037523; VOC.
DR SUPFAM; SSF54593; SSF54593; 1.
DR TIGRFAMs; TIGR03081; metmalonyl_epim; 1.
DR PROSITE; PS51819; VOC; 1.
PE 3: Inferred from homology;
KW Cobalt; Isomerase; Metal-binding; Mitochondrion; Reference proteome;
KW Transit peptide.
FT TRANSIT 1..23
FT /note="Mitochondrion"
FT /evidence="ECO:0000255"
FT CHAIN 24..166
FT /note="Methylmalonyl-CoA epimerase, mitochondrial"
FT /id="PRO_0000327653"
FT DOMAIN 37..166
FT /note="VOC"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01163"
FT BINDING 40
FT /ligand="Co(2+)"
FT /ligand_id="ChEBI:CHEBI:48828"
FT /evidence="ECO:0000250"
FT BINDING 112
FT /ligand="Co(2+)"
FT /ligand_id="ChEBI:CHEBI:48828"
FT /evidence="ECO:0000250"
FT BINDING 162
FT /ligand="Co(2+)"
FT /ligand_id="ChEBI:CHEBI:48828"
FT /evidence="ECO:0000250"
SQ SEQUENCE 166 AA; 18063 MW; 3C249ABF2248C9B0 CRC64;
MFKQLIKTTL TNSRSFSTNT GSGSKIGNII GKIGIGKLNH VAIATPNLQE SMDLYKNVFG
ADVSEPVNQV EHGVTTVFVG LENTKIELLH PFGDKSPIEN FLKKNPSGGI HHICLEVDDI
HHAVKTLLAE NVRIIDPTPK IGAHGKPVVF LHPKSMNGVL VELEEK
