MCES2_ORYSJ
ID MCES2_ORYSJ Reviewed; 339 AA.
AC Q6K833; B7EDQ2;
DT 28-NOV-2006, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 98.
DE RecName: Full=mRNA cap guanine-N7 methyltransferase 2;
DE EC=2.1.1.56;
DE AltName: Full=mRNA (guanine-N(7))-methyltransferase 2;
DE AltName: Full=mRNA cap methyltransferase 2;
GN OrderedLocusNames=Os02g0780600, LOC_Os02g54000; ORFNames=OJ1369_G08.2;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
CC -!- FUNCTION: mRNA-capping methyltransferase that methylates the N7
CC position of the added guanosine to the 5'-cap structure of mRNAs. Binds
CC RNA containing 5'-terminal GpppC (By similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 5'-end (5'-triphosphoguanosine)-(ribonucleoside) in mRNA +
CC S-adenosyl-L-methionine = a 5'-end (N(7)-methyl 5'-
CC triphosphoguanosine)-ribonucleoside in mRNA + S-adenosyl-L-
CC homocysteine; Xref=Rhea:RHEA:67008, Rhea:RHEA-COMP:17166, Rhea:RHEA-
CC COMP:17167, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:156461,
CC ChEBI:CHEBI:167617; EC=2.1.1.56; Evidence={ECO:0000255|PROSITE-
CC ProRule:PRU00895};
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC superfamily. mRNA cap 0 methyltransferase family. {ECO:0000255|PROSITE-
CC ProRule:PRU00895}.
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DR EMBL; AP004257; BAD19434.1; -; Genomic_DNA.
DR EMBL; AP008208; BAF10219.1; -; Genomic_DNA.
DR EMBL; AP014958; BAS81216.1; -; Genomic_DNA.
DR EMBL; AK067608; BAG90499.1; -; mRNA.
DR EMBL; AK121526; -; NOT_ANNOTATED_CDS; mRNA.
DR RefSeq; XP_015623577.1; XM_015768091.1.
DR AlphaFoldDB; Q6K833; -.
DR SMR; Q6K833; -.
DR STRING; 4530.OS02T0780600-01; -.
DR PaxDb; Q6K833; -.
DR PRIDE; Q6K833; -.
DR EnsemblPlants; Os02t0780600-01; Os02t0780600-01; Os02g0780600.
DR EnsemblPlants; Os02t0780600-02; Os02t0780600-02; Os02g0780600.
DR GeneID; 4330921; -.
DR Gramene; Os02t0780600-01; Os02t0780600-01; Os02g0780600.
DR Gramene; Os02t0780600-02; Os02t0780600-02; Os02g0780600.
DR KEGG; osa:4330921; -.
DR eggNOG; ENOG502QR4R; Eukaryota.
DR HOGENOM; CLU_045607_0_0_1; -.
DR InParanoid; Q6K833; -.
DR OMA; YLDEMEW; -.
DR OrthoDB; 1390749at2759; -.
DR Proteomes; UP000000763; Chromosome 2.
DR Proteomes; UP000059680; Chromosome 2.
DR Genevisible; Q6K833; OS.
DR GO; GO:0005845; C:mRNA cap binding complex; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0004482; F:mRNA (guanine-N7-)-methyltransferase activity; IBA:GO_Central.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0006370; P:7-methylguanosine mRNA capping; IBA:GO_Central.
DR Gene3D; 3.40.50.150; -; 1.
DR InterPro; IPR004971; mRNA_G-N7_MeTrfase_dom.
DR InterPro; IPR039753; RG7MT1.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR PANTHER; PTHR12189; PTHR12189; 1.
DR Pfam; PF03291; Pox_MCEL; 1.
DR SUPFAM; SSF53335; SSF53335; 1.
DR PROSITE; PS51562; RNA_CAP0_MT; 1.
PE 2: Evidence at transcript level;
KW Methyltransferase; mRNA capping; mRNA processing; Nucleus;
KW Reference proteome; RNA-binding; S-adenosyl-L-methionine; Transferase.
FT CHAIN 1..339
FT /note="mRNA cap guanine-N7 methyltransferase 2"
FT /id="PRO_0000260153"
FT DOMAIN 1..284
FT /note="mRNA cap 0 methyltransferase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00895"
FT REGION 314..339
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 14
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00895"
FT BINDING 54
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00895"
FT BINDING 82..83
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00895"
FT SITE 41
FT /note="mRNA cap binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00895"
FT SITE 269
FT /note="mRNA cap binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00895"
FT CONFLICT 116
FT /note="E -> V (in Ref. 4; AK121526)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 339 AA; 38388 MW; 136DA11E7D0807CB CRC64;
MAVTPHHRLY EFAKTALIKI FAFPYATVCD LYCDGGVDTD KWGDAQIGHY IGIDASASGV
NDARELWESR KKLFTSEFIE LDPSADDFEA QMQEKGIQAD IVCCMQHLQL CFESEEHAQK
LLNNVSSLLK PGGYFVGIIP DSSTIWTKYQ KNVEASHNKG LKTVPNSIRS ENYVITFEVE
EEKFPFFGKK YQLKFANESM FENHCLVHFP SFMRLAREAG LEYVEIQNLT EFYDDNRTQF
APLLGGYGSS LVDPRGKLVA RSFDILGLYS TFVFQKPDPD AMPPIVTPEL HDPENDQEEE
WLWTQQASMD DGRVSRTDIL PPADNEKGIL GPGPADMRL
