MCE_RDVA
ID MCE_RDVA Reviewed; 801 AA.
AC P14583;
DT 01-JAN-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1990, sequence version 1.
DT 29-SEP-2021, entry version 65.
DE RecName: Full=Putative mRNA-capping enzyme P5;
DE AltName: Full=Structural protein 5;
DE AltName: Full=mRNA guanylyltransferase;
DE EC=2.7.7.50;
OS Rice dwarf virus (isolate Akita) (RDV).
OC Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes;
OC Reovirales; Reoviridae; Sedoreovirinae; Phytoreovirus.
OX NCBI_TaxID=142803;
OH NCBI_TaxID=114194; Alopecurus aequalis.
OH NCBI_TaxID=90397; Echinochloa crus-galli (Barnyard grass) (Panicum crus-galli).
OH NCBI_TaxID=94400; Nephotettix cincticeps (Green rice leafhopper) (Selenocephalus cincticeps).
OH NCBI_TaxID=4530; Oryza sativa (Rice).
OH NCBI_TaxID=147271; Paspalum.
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX PubMed=2587221; DOI=10.1093/nar/17.21.8858;
RA Suzuki N., Watanabe Y., Kusano T., Kitagawa Y.;
RT "Nucleotide sequence of rice dwarf virus segment 5.";
RL Nucleic Acids Res. 17:8858-8858(1989).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX PubMed=2219732; DOI=10.1016/0042-6822(90)90313-g;
RA Suzuki N., Watanabe Y., Kusano T., Kitagawa Y.;
RT "Sequence analysis of rice dwarf phytoreovirus genome segments S4, S5, and
RT S6: comparison with the equivalent wound tumor virus segments.";
RL Virology 179:446-454(1990).
CC -!- FUNCTION: Enzyme involved in mRNA capping (Potential). Binds to GTP and
CC might have guanylyltransferase activity. Together with the RNA-directed
CC RNA polymerase P1 and protein P7, forms an transcriptional complex
CC positioned near the channels situated at each of the five-fold vertices
CC of the core (By similarity). {ECO:0000250, ECO:0000305}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 5'-end diphospho-ribonucleoside in mRNA + GTP + H(+) = a 5'-
CC end (5'-triphosphoguanosine)-(ribonucleoside) in mRNA + diphosphate;
CC Xref=Rhea:RHEA:67012, Rhea:RHEA-COMP:17165, Rhea:RHEA-COMP:17166,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:33019, ChEBI:CHEBI:37565,
CC ChEBI:CHEBI:167616, ChEBI:CHEBI:167617; EC=2.7.7.50;
CC -!- PATHWAY: mRNA processing; mRNA capping.
CC -!- SUBCELLULAR LOCATION: Virion {ECO:0000250}. Host cytoplasm
CC {ECO:0000250}. Note=Located inside the inner capsid. Found in the
CC interior of spherical cytoplasmic structures, called virus factories,
CC that appear early after infection and are the site of viral replication
CC and packaging. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the phytoreovirus protein P5 family.
CC {ECO:0000305}.
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DR EMBL; D90033; BAA14081.1; -; Genomic_RNA.
DR PIR; S06751; MWXRR5.
DR PDB; 5X6X; X-ray; 2.10 A; A/B/C/D=1-801.
DR PDB; 5X6Y; X-ray; 2.10 A; A/B/C/D=1-801.
DR PDB; 5X6Z; X-ray; 2.10 A; A/B/C/D=1-801.
DR PDB; 5X70; X-ray; 3.30 A; A/B/C/D=1-801.
DR PDB; 5X71; X-ray; 2.57 A; A/B=1-801.
DR PDBsum; 5X6X; -.
DR PDBsum; 5X6Y; -.
DR PDBsum; 5X6Z; -.
DR PDBsum; 5X70; -.
DR PDBsum; 5X71; -.
DR SMR; P14583; -.
DR UniPathway; UPA00922; -.
DR GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0004484; F:mRNA guanylyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0006370; P:7-methylguanosine mRNA capping; IEA:UniProtKB-UniPathway.
DR CDD; cd20759; capping_2-OMTase_Phytoreovirus; 1.
DR InterPro; IPR044310; P5_Phytoreov.
PE 1: Evidence at protein level;
KW 3D-structure; GTP-binding; Host cytoplasm; mRNA capping; mRNA processing;
KW Nucleotide-binding; Nucleotidyltransferase; RNA-binding; Transferase;
KW Virion.
FT CHAIN 1..801
FT /note="Putative mRNA-capping enzyme P5"
FT /id="PRO_0000222786"
FT STRAND 7..9
FT /evidence="ECO:0007829|PDB:5X6X"
FT TURN 12..14
FT /evidence="ECO:0007829|PDB:5X6X"
FT HELIX 17..27
FT /evidence="ECO:0007829|PDB:5X6X"
FT STRAND 33..37
FT /evidence="ECO:0007829|PDB:5X6X"
FT HELIX 41..55
FT /evidence="ECO:0007829|PDB:5X6X"
FT STRAND 58..62
FT /evidence="ECO:0007829|PDB:5X6X"
FT STRAND 64..66
FT /evidence="ECO:0007829|PDB:5X6X"
FT HELIX 68..80
FT /evidence="ECO:0007829|PDB:5X6X"
FT TURN 81..84
FT /evidence="ECO:0007829|PDB:5X6X"
FT STRAND 85..87
FT /evidence="ECO:0007829|PDB:5X6Y"
FT STRAND 89..93
FT /evidence="ECO:0007829|PDB:5X6X"
FT TURN 97..99
FT /evidence="ECO:0007829|PDB:5X6X"
FT HELIX 102..111
FT /evidence="ECO:0007829|PDB:5X6X"
FT STRAND 115..118
FT /evidence="ECO:0007829|PDB:5X6X"
FT HELIX 127..139
FT /evidence="ECO:0007829|PDB:5X6X"
FT STRAND 149..151
FT /evidence="ECO:0007829|PDB:5X6X"
FT HELIX 158..161
FT /evidence="ECO:0007829|PDB:5X6X"
FT TURN 164..168
FT /evidence="ECO:0007829|PDB:5X6X"
FT HELIX 174..196
FT /evidence="ECO:0007829|PDB:5X6X"
FT HELIX 201..203
FT /evidence="ECO:0007829|PDB:5X6X"
FT STRAND 205..210
FT /evidence="ECO:0007829|PDB:5X6X"
FT HELIX 215..221
FT /evidence="ECO:0007829|PDB:5X6X"
FT STRAND 225..232
FT /evidence="ECO:0007829|PDB:5X6X"
FT STRAND 237..240
FT /evidence="ECO:0007829|PDB:5X6X"
FT STRAND 243..246
FT /evidence="ECO:0007829|PDB:5X6X"
FT HELIX 267..277
FT /evidence="ECO:0007829|PDB:5X6X"
FT STRAND 281..287
FT /evidence="ECO:0007829|PDB:5X6X"
FT HELIX 292..296
FT /evidence="ECO:0007829|PDB:5X6X"
FT STRAND 303..307
FT /evidence="ECO:0007829|PDB:5X6X"
FT HELIX 313..323
FT /evidence="ECO:0007829|PDB:5X6X"
FT STRAND 326..330
FT /evidence="ECO:0007829|PDB:5X6X"
FT HELIX 346..349
FT /evidence="ECO:0007829|PDB:5X6X"
FT STRAND 352..358
FT /evidence="ECO:0007829|PDB:5X6X"
FT STRAND 363..365
FT /evidence="ECO:0007829|PDB:5X6X"
FT HELIX 366..380
FT /evidence="ECO:0007829|PDB:5X6X"
FT STRAND 384..395
FT /evidence="ECO:0007829|PDB:5X6X"
FT STRAND 401..411
FT /evidence="ECO:0007829|PDB:5X6X"
FT STRAND 421..425
FT /evidence="ECO:0007829|PDB:5X6X"
FT STRAND 429..435
FT /evidence="ECO:0007829|PDB:5X6X"
FT HELIX 437..448
FT /evidence="ECO:0007829|PDB:5X6X"
FT HELIX 452..456
FT /evidence="ECO:0007829|PDB:5X6X"
FT HELIX 459..463
FT /evidence="ECO:0007829|PDB:5X6X"
FT HELIX 464..466
FT /evidence="ECO:0007829|PDB:5X6X"
FT STRAND 467..470
FT /evidence="ECO:0007829|PDB:5X6X"
FT HELIX 472..474
FT /evidence="ECO:0007829|PDB:5X6X"
FT TURN 478..480
FT /evidence="ECO:0007829|PDB:5X71"
FT STRAND 482..486
FT /evidence="ECO:0007829|PDB:5X6X"
FT HELIX 487..490
FT /evidence="ECO:0007829|PDB:5X6X"
FT HELIX 496..508
FT /evidence="ECO:0007829|PDB:5X6X"
FT STRAND 512..519
FT /evidence="ECO:0007829|PDB:5X6X"
FT HELIX 520..528
FT /evidence="ECO:0007829|PDB:5X6X"
FT TURN 534..536
FT /evidence="ECO:0007829|PDB:5X6X"
FT STRAND 537..540
FT /evidence="ECO:0007829|PDB:5X6X"
FT STRAND 543..546
FT /evidence="ECO:0007829|PDB:5X6X"
FT STRAND 555..557
FT /evidence="ECO:0007829|PDB:5X6X"
FT HELIX 561..566
FT /evidence="ECO:0007829|PDB:5X6X"
FT STRAND 569..571
FT /evidence="ECO:0007829|PDB:5X6X"
FT HELIX 574..580
FT /evidence="ECO:0007829|PDB:5X6X"
FT TURN 581..584
FT /evidence="ECO:0007829|PDB:5X6X"
FT STRAND 590..592
FT /evidence="ECO:0007829|PDB:5X6X"
FT HELIX 595..598
FT /evidence="ECO:0007829|PDB:5X6X"
FT STRAND 600..605
FT /evidence="ECO:0007829|PDB:5X6X"
FT HELIX 606..612
FT /evidence="ECO:0007829|PDB:5X6X"
FT STRAND 613..615
FT /evidence="ECO:0007829|PDB:5X6Y"
FT HELIX 618..620
FT /evidence="ECO:0007829|PDB:5X6X"
FT TURN 623..627
FT /evidence="ECO:0007829|PDB:5X6X"
FT HELIX 628..637
FT /evidence="ECO:0007829|PDB:5X6X"
FT HELIX 641..658
FT /evidence="ECO:0007829|PDB:5X6X"
FT HELIX 663..665
FT /evidence="ECO:0007829|PDB:5X6X"
FT STRAND 666..668
FT /evidence="ECO:0007829|PDB:5X6X"
FT STRAND 671..673
FT /evidence="ECO:0007829|PDB:5X6X"
FT STRAND 675..680
FT /evidence="ECO:0007829|PDB:5X6X"
FT STRAND 682..684
FT /evidence="ECO:0007829|PDB:5X6X"
FT STRAND 691..693
FT /evidence="ECO:0007829|PDB:5X6X"
FT STRAND 697..699
FT /evidence="ECO:0007829|PDB:5X6X"
FT HELIX 703..711
FT /evidence="ECO:0007829|PDB:5X6X"
FT TURN 712..714
FT /evidence="ECO:0007829|PDB:5X6X"
FT HELIX 719..728
FT /evidence="ECO:0007829|PDB:5X6X"
FT STRAND 737..739
FT /evidence="ECO:0007829|PDB:5X70"
FT HELIX 741..744
FT /evidence="ECO:0007829|PDB:5X6X"
FT STRAND 753..755
FT /evidence="ECO:0007829|PDB:5X6X"
FT HELIX 761..778
FT /evidence="ECO:0007829|PDB:5X6X"
FT TURN 779..781
FT /evidence="ECO:0007829|PDB:5X6X"
FT HELIX 785..798
FT /evidence="ECO:0007829|PDB:5X6X"
SQ SEQUENCE 801 AA; 90534 MW; C58B06DD78C3EE36 CRC64;
MSNPDYCIPN FSQTVNERTI IDIFTICRYR SPLVVFCLSH NELAKKYAQD VSMSSGTHVH
IIDGSVEITA SLYRTFRTIA TQLLGRMQIV VFVTVDKSVV STQVMKSIAW AFRGSFVELR
NQSVDSSTLV SKLENLVSFA PLYNVPKCGP DYYGPTVYSE LLSLATNART HWYATIDYSM
FTRSVLTGFI AKYFNEEAVP IDKRIVSIVG YNPPYVWTCL RHGIRPTYIE KSLPNPGGKG
PFGLILPVIN ELVLKSKVKY VMHNPQIKLL CLDTFMLSTS MNILYIGAYP ATHLLSLQLN
GWTILAFDPK ITSDWTDAMA KATGAKVIGV NKEFDFKSFS VQANQLNMFQ NSKLSVIDDT
WVETDYEKFQ AEKQAYFEWL IDRTSIDVRL ISMKWNRSKD TSVSHLLALL PQPYGASIRE
MRAFFHKKGA SDIKILAAET EKYMDDFTAM SVSDQINTQK FMHCMITTVG DALKMDLDGG
RAVIASYSLS NSSNPKERVL KFLSDANKAK AMVVFGAPNT HRLAYAKKVG LVLDSAIKMS
KDLITFSNPT GRRWRDYGYS QSELYDAGYV EITIDQMVAY SSDVYNGVGY FANSTYNDLF
SWYIPKWYVH KRMLMQDIRL SPAALVKCFT TLIRNICYVP HETYYRFRGI LVDKYLRSKN
VDPSQYSIVG SGSKTFTVLN HFEVPHECGP LVFEASTDVN ISGHLLSLAI AAHFVASPMI
LWAEQMKYMA VDRMLPPNLD KSLFFDNKVT PSGALQRWHS REEVLLAAEI CESYAAMMLN
NKHSPDIIGT LKSAINLVFK I
