MCFI_DICDI
ID MCFI_DICDI Reviewed; 338 AA.
AC Q86I81; Q55AF5;
DT 13-OCT-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 116.
DE RecName: Full=Mitochondrial substrate carrier family protein I;
DE AltName: Full=Mitochondrial folate transporter A;
GN Name=mcfI; Synonyms=mftA; ORFNames=DDB_G0271922;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND INDUCTION.
RX PubMed=15075287; DOI=10.1128/ec.3.2.564-566.2004;
RA Escalante R., Iranfar N., Sastre L., Loomis W.F.;
RT "Identification of genes dependent on the MADS box transcription factor
RT SrfA in Dictyostelium discoideum development.";
RL Eukaryot. Cell 3:564-566(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=12097910; DOI=10.1038/nature00847;
RA Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T.,
RA Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R.,
RA Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A.,
RA Noegel A.A.;
RT "Sequence and analysis of chromosome 2 of Dictyostelium discoideum.";
RL Nature 418:79-85(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [4]
RP REVIEW.
RX PubMed=17442478; DOI=10.1016/j.biochi.2007.03.004;
RA Satre M., Mattei S., Aubry L., Gaudet P., Pelosi L., Brandolin G.,
RA Klein G.;
RT "Mitochondrial carrier family: repertoire and peculiarities of the cellular
RT slime mould Dictyostelium discoideum.";
RL Biochimie 89:1058-1069(2007).
CC -!- FUNCTION: Mitochondrial solute carriers shuttle metabolites,
CC nucleotides, and cofactors through the mitochondrial inner membrane.
CC Transports folate across the inner membranes of mitochondria (By
CC similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane {ECO:0000250};
CC Multi-pass membrane protein {ECO:0000250}.
CC -!- INDUCTION: Regulated by the MADS-box transcription factor srfA during
CC development. {ECO:0000269|PubMed:15075287}.
CC -!- SIMILARITY: Belongs to the mitochondrial carrier (TC 2.A.29) family.
CC {ECO:0000305}.
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DR EMBL; AY392437; AAQ98878.1; -; Genomic_DNA.
DR EMBL; AAFI02000007; EAL71485.1; -; Genomic_DNA.
DR RefSeq; XP_645399.1; XM_640307.1.
DR AlphaFoldDB; Q86I81; -.
DR SMR; Q86I81; -.
DR PaxDb; Q86I81; -.
DR EnsemblProtists; EAL71485; EAL71485; DDB_G0271922.
DR GeneID; 8618221; -.
DR KEGG; ddi:DDB_G0271922; -.
DR dictyBase; DDB_G0271922; mcfI.
DR eggNOG; KOG0764; Eukaryota.
DR HOGENOM; CLU_015166_6_4_1; -.
DR InParanoid; Q86I81; -.
DR OMA; GLYFLWY; -.
DR PhylomeDB; Q86I81; -.
DR Reactome; R-DDI-196757; Metabolism of folate and pterines.
DR PRO; PR:Q86I81; -.
DR Proteomes; UP000002195; Chromosome 2.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005739; C:mitochondrion; IBA:GO_Central.
DR GO; GO:0015230; F:FAD transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0008517; F:folic acid transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0022857; F:transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:1904947; P:folate import into mitochondrion; IBA:GO_Central.
DR GO; GO:0055085; P:transmembrane transport; IBA:GO_Central.
DR Gene3D; 1.50.40.10; -; 1.
DR InterPro; IPR018108; Mitochondrial_sb/sol_carrier.
DR InterPro; IPR023395; Mt_carrier_dom_sf.
DR InterPro; IPR044712; SLC25A32-like.
DR PANTHER; PTHR45683; PTHR45683; 1.
DR Pfam; PF00153; Mito_carr; 3.
DR SUPFAM; SSF103506; SSF103506; 1.
DR PROSITE; PS50920; SOLCAR; 3.
PE 2: Evidence at transcript level;
KW Membrane; Mitochondrion; Mitochondrion inner membrane; Reference proteome;
KW Repeat; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..338
FT /note="Mitochondrial substrate carrier family protein I"
FT /id="PRO_0000385523"
FT TOPO_DOM 1..24
FT /note="Mitochondrial intermembrane"
FT /evidence="ECO:0000250"
FT TRANSMEM 25..45
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 46..109
FT /note="Mitochondrial matrix"
FT /evidence="ECO:0000250"
FT TRANSMEM 110..130
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 131..158
FT /note="Mitochondrial intermembrane"
FT /evidence="ECO:0000250"
FT TRANSMEM 159..179
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 180..203
FT /note="Mitochondrial matrix"
FT /evidence="ECO:0000250"
FT TRANSMEM 204..224
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 225..253
FT /note="Mitochondrial intermembrane"
FT /evidence="ECO:0000250"
FT TRANSMEM 254..274
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 275..307
FT /note="Mitochondrial matrix"
FT /evidence="ECO:0000250"
FT TRANSMEM 308..328
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 329..338
FT /note="Mitochondrial intermembrane"
FT /evidence="ECO:0000250"
FT REPEAT 25..139
FT /note="Solcar 1"
FT REPEAT 153..235
FT /note="Solcar 2"
FT REPEAT 248..332
FT /note="Solcar 3"
SQ SEQUENCE 338 AA; 36655 MW; E58779BDF7546CA4 CRC64;
MVNGEEYVET PNLIHDEETR KDVKFNLGIE LLAGTLAGVS SCILFYPLEC VEAKLQVQSS
STAVAATMLG LKKNGGSGSG SSSSSSISHQ TPNGPIAMAK SILRNEGFKG FYQGVSPTIL
GNAVNWGVYF SIYRATNHWW NSTDINGNQY QGPAWVGHSV SAITAGVITT AIVNPFWVLK
IRLATSKKYS GMKHAFQSIL RSEGVGGFWK GVGVSFIGVS EGLFQFVSYE YILNQMKESN
LKMNGGELSV GNYLFAGGTA RLIAGVLTYP YLLIRSSLQS ETCPYKSMSE AVKGIYKTNG
IKGFYKGIGP NLARSIPPAA FMLYIVEFFR DTLTNFSQ
