MCFN_DICDI
ID MCFN_DICDI Reviewed; 298 AA.
AC Q54BF6;
DT 13-OCT-2009, integrated into UniProtKB/Swiss-Prot.
DT 18-MAR-2008, sequence version 2.
DT 25-MAY-2022, entry version 105.
DE RecName: Full=Mitochondrial substrate carrier family protein N;
DE AltName: Full=Solute carrier family 25 member 3 homolog;
GN Name=mcfN; Synonyms=slc25a3; ORFNames=DDB_G0293646;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [2]
RP PROTEIN SEQUENCE OF 134-142 AND 261-269, AND IDENTIFICATION BY MASS
RP SPECTROMETRY.
RA Bienvenut W.V., Veltman D.M., Insall R.H.;
RL Submitted (JAN-2010) to UniProtKB.
RN [3]
RP REVIEW.
RX PubMed=17442478; DOI=10.1016/j.biochi.2007.03.004;
RA Satre M., Mattei S., Aubry L., Gaudet P., Pelosi L., Brandolin G.,
RA Klein G.;
RT "Mitochondrial carrier family: repertoire and peculiarities of the cellular
RT slime mould Dictyostelium discoideum.";
RL Biochimie 89:1058-1069(2007).
CC -!- FUNCTION: Mitochondrial solute carriers shuttle metabolites,
CC nucleotides, and cofactors through the mitochondrial inner membrane.
CC Transports phosphate groups from the cytosol to the mitochondrial
CC matrix. Phosphate is cotransported with H(+) (By similarity).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane; Multi-pass membrane
CC protein.
CC -!- SIMILARITY: Belongs to the mitochondrial carrier (TC 2.A.29) family.
CC {ECO:0000305}.
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DR EMBL; AAFI02000218; EAL60635.2; -; Genomic_DNA.
DR RefSeq; XP_629073.2; XM_629071.2.
DR AlphaFoldDB; Q54BF6; -.
DR SMR; Q54BF6; -.
DR IntAct; Q54BF6; 1.
DR STRING; 44689.DDB0233888; -.
DR PaxDb; Q54BF6; -.
DR EnsemblProtists; EAL60635; EAL60635; DDB_G0293646.
DR GeneID; 8629365; -.
DR KEGG; ddi:DDB_G0293646; -.
DR dictyBase; DDB_G0293646; mcfN.
DR eggNOG; KOG0767; Eukaryota.
DR HOGENOM; CLU_039456_1_0_1; -.
DR InParanoid; Q54BF6; -.
DR OMA; VKIQTVP; -.
DR PhylomeDB; Q54BF6; -.
DR PRO; PR:Q54BF6; -.
DR Proteomes; UP000002195; Chromosome 6.
DR GO; GO:0031305; C:integral component of mitochondrial inner membrane; IBA:GO_Central.
DR GO; GO:0005739; C:mitochondrion; ISS:dictyBase.
DR GO; GO:0005315; F:inorganic phosphate transmembrane transporter activity; ISS:dictyBase.
DR GO; GO:1990547; P:mitochondrial phosphate ion transmembrane transport; IEA:InterPro.
DR GO; GO:0035435; P:phosphate ion transmembrane transport; IBA:GO_Central.
DR GO; GO:0006817; P:phosphate ion transport; ISS:dictyBase.
DR Gene3D; 1.50.40.10; -; 1.
DR InterPro; IPR002067; Mit_carrier.
DR InterPro; IPR018108; Mitochondrial_sb/sol_carrier.
DR InterPro; IPR023395; Mt_carrier_dom_sf.
DR InterPro; IPR044677; Pic2/Mir1-like.
DR PANTHER; PTHR45671; PTHR45671; 1.
DR Pfam; PF00153; Mito_carr; 3.
DR PRINTS; PR00926; MITOCARRIER.
DR SUPFAM; SSF103506; SSF103506; 1.
DR PROSITE; PS50920; SOLCAR; 3.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Membrane; Mitochondrion;
KW Mitochondrion inner membrane; Reference proteome; Repeat; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..298
FT /note="Mitochondrial substrate carrier family protein N"
FT /id="PRO_0000385534"
FT TOPO_DOM 1..13
FT /note="Mitochondrial intermembrane"
FT /evidence="ECO:0000250"
FT TRANSMEM 14..34
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 35..60
FT /note="Mitochondrial matrix"
FT /evidence="ECO:0000250"
FT TRANSMEM 61..81
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 82..105
FT /note="Mitochondrial intermembrane"
FT /evidence="ECO:0000250"
FT TRANSMEM 106..126
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 127..162
FT /note="Mitochondrial matrix"
FT /evidence="ECO:0000250"
FT TRANSMEM 163..179
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 180..208
FT /note="Mitochondrial intermembrane"
FT /evidence="ECO:0000250"
FT TRANSMEM 209..229
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 230..262
FT /note="Mitochondrial matrix"
FT /evidence="ECO:0000250"
FT TRANSMEM 263..283
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 284..298
FT /note="Mitochondrial intermembrane"
FT /evidence="ECO:0000250"
FT REPEAT 8..92
FT /note="Solcar 1"
FT REPEAT 104..188
FT /note="Solcar 2"
FT REPEAT 207..290
FT /note="Solcar 3"
SQ SEQUENCE 298 AA; 31687 MW; EB628AFDECC3DECE CRC64;
MAGDLTPSLF LKYGFGGALS CSITHSLVVP LDVVKTLLQT NPGKYTGMMN GFSTVIKEQG
PSGLLQGLGP TAVGYALQGF LKFGFYEVFK KTYADAVGEK ADQFRIPIWL AASATAEVIA
DIALCPNEAV RIRLVAEPTF AKSPVEAFGK IFKQEGVLGF YKGLPPILLK QVPYTMAKFA
VFEFTAENVY KGLAASGKPK ESLTDGQKLS VSLGSGIVAG IVAAIVSQPA DTILSKINQE
KTDGGVVKAI GNIMRRLGVR GLFLGLPTRC FMVGTLTAGQ FFIYDGIKQM LGLTPAKK
