MCH2_YEAST
ID MCH2_YEAST Reviewed; 473 AA.
AC P36032; D6VWY2;
DT 01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT 21-SEP-2011, sequence version 2.
DT 03-AUG-2022, entry version 152.
DE RecName: Full=Probable transporter MCH2;
GN Name=MCH2; OrderedLocusNames=YKL221W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8091865; DOI=10.1002/yea.320100011;
RA Alexandraki D., Tzermia M.;
RT "Sequencing of a 13.2 kb segment next to the left telomere of yeast
RT chromosome XI revealed five open reading frames and recent recombination
RT events with the right arms of chromosomes III and V.";
RL Yeast 10:S81-S91(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8196765; DOI=10.1038/369371a0;
RA Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V.,
RA Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P.,
RA Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L.,
RA Daignan-Fornier B., del Rey F., Dion C., Domdey H., Duesterhoeft A.,
RA Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H.,
RA Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L.,
RA Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M.,
RA Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H.,
RA Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J.,
RA Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H.,
RA Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J.,
RA Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S.,
RA Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F.,
RA Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R.,
RA Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W.,
RA Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M.,
RA Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C.,
RA Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H.,
RA Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L.,
RA van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S.,
RA von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M.,
RA Becker I., Mewes H.-W.;
RT "Complete DNA sequence of yeast chromosome XI.";
RL Nature 369:371-378(1994).
RN [3]
RP GENOME REANNOTATION, AND SEQUENCE REVISION TO 342.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP LACK OF FUNCTION AS A MONOCARBOXYLATE TRANSPORTER.
RX PubMed=11536335; DOI=10.1002/yea.763;
RA Makuc J., Paiva S., Schauen M., Kramer R., Andre B., Casal M., Leao C.,
RA Boles E.;
RT "The putative monocarboxylate permeases of the yeast Saccharomyces
RT cerevisiae do not transport monocarboxylic acids across the plasma
RT membrane.";
RL Yeast 18:1131-1143(2001).
RN [5]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 208353 / W303-1A;
RX PubMed=16847258; DOI=10.1073/pnas.0604075103;
RA Kim H., Melen K., Oesterberg M., von Heijne G.;
RT "A global topology map of the Saccharomyces cerevisiae membrane proteome.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006).
CC -!- FUNCTION: Probable transporter. Does not act in the transport of
CC monocarboxylic acids across the plasma membrane.
CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily.
CC Monocarboxylate porter (TC 2.A.1.13) family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; X75950; CAA53552.1; -; Genomic_DNA.
DR EMBL; Z28221; CAA82066.1; -; Genomic_DNA.
DR EMBL; BK006944; DAA08948.2; -; Genomic_DNA.
DR PIR; S38065; S38065.
DR RefSeq; NP_012701.2; NM_001179786.2.
DR AlphaFoldDB; P36032; -.
DR SMR; P36032; -.
DR BioGRID; 33944; 42.
DR DIP; DIP-4126N; -.
DR IntAct; P36032; 1.
DR MINT; P36032; -.
DR STRING; 4932.YKL221W; -.
DR TCDB; 2.A.1.13.18; the major facilitator superfamily (mfs).
DR PaxDb; P36032; -.
DR PRIDE; P36032; -.
DR EnsemblFungi; YKL221W_mRNA; YKL221W; YKL221W.
DR GeneID; 853659; -.
DR KEGG; sce:YKL221W; -.
DR SGD; S000001704; MCH2.
DR VEuPathDB; FungiDB:YKL221W; -.
DR eggNOG; KOG2504; Eukaryota.
DR HOGENOM; CLU_001265_1_2_1; -.
DR InParanoid; P36032; -.
DR OMA; LSYRIWA; -.
DR BioCyc; YEAST:G3O-31976-MON; -.
DR Reactome; R-SCE-352230; Amino acid transport across the plasma membrane.
DR Reactome; R-SCE-879518; Transport of organic anions.
DR PRO; PR:P36032; -.
DR Proteomes; UP000002311; Chromosome XI.
DR RNAct; P36032; protein.
DR GO; GO:0071627; C:integral component of fungal-type vacuolar membrane; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IBA:GO_Central.
DR GO; GO:0016020; C:membrane; ISM:SGD.
DR GO; GO:0015293; F:symporter activity; IEA:UniProtKB-KW.
DR Gene3D; 1.20.1250.20; -; 2.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR036259; MFS_trans_sf.
DR Pfam; PF07690; MFS_1; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
PE 1: Evidence at protein level;
KW Glycoprotein; Membrane; Reference proteome; Symport; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..473
FT /note="Probable transporter MCH2"
FT /id="PRO_0000211403"
FT TOPO_DOM 1..37
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 38..58
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 59..83
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 84..105
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 106..111
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 112..135
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 136..141
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 142..163
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 164..169
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 170..186
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 187..200
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 201..220
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 221..243
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 244..268
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 269..286
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 287..304
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 305..312
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 313..332
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 333..342
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 343..362
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 363..370
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 371..394
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 395..408
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 409..433
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 434..473
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CARBOHYD 72
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 342
FT /note="A -> R (in Ref. 1; CAA53552 and 2; CAA82066)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 473 AA; 52257 MW; 6C58D5DB3BAB5510 CRC64;
MSEERHEDHH RDVENKLNLN GKDDINGNTS ISIEVPDGGY GWFILLAFIL YNFSTWGANS
GYAIYLAHYL ENNTFAGGSK LDYASIGGLA FSCGLFFAPV ITWLYHIFSI QFIIGLGILF
QGAALLLAAF SVTLWEIYLT QGVLIGFGLA FIFIPSVTLI PLWFRNKRSL ASGIGTAGSG
LGGIVFNLGM QSILQKRGVK WALIAQCIIC TSLSTIALML TRTTHQGLRQ HKRSYKFELL
DYDVLSNFAV WLLFGFVSFA MLGYVVLLYS LSDFTVSLGY TSKQGSYVSC MVSVGSLLGR
PIVGHIADKY GSLTVGMILH LVMAILCWAM WIPCKNLATA IAFGLLVGSI MGTIWPTIAS
IVTRIVGLQK LPGTFGSTWI FMAAFALVAP IIGLELRSTD TNGNDYYRTA IFVGFAYFGV
SLCQWLLRGF IIARDEIAVR EAYSADQNEL HLNVKLSHMS KCLFRYKQLP RRV
