ID   MCHB_ECOLX              Reviewed;          75 AA.
AC   P62530; Q9RM53;
DT   19-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT   19-JUL-2004, sequence version 1.
DT   25-MAY-2022, entry version 59.
DE   RecName: Full=Microcin H47;
DE            Short=MccH47;
DE   Flags: Precursor;
GN   Name=mchB;
OS   Escherichia coli.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=H47;
RX   PubMed=10471561; DOI=10.1128/aac.43.9.2176;
RA   Rodriguez E., Gaggero C., Lavina M.;
RT   "The structural gene for microcin H47 encodes a peptide precursor with
RT   antibiotic activity.";
RL   Antimicrob. Agents Chemother. 43:2176-2182(1999).
RN   [2]
RP   CHARACTERIZATION.
RC   STRAIN=H47;
RX   PubMed=11181394; DOI=10.1128/aac.45.3.969-972.2001;
RA   Azpiroz M.F., Rodriguez E., Lavina M.;
RT   "The structure, function, and origin of the microcin H47 ATP-binding
RT   cassette exporter indicate its relatedness to that of colicin V.";
RL   Antimicrob. Agents Chemother. 45:969-972(2001).
CC   -!- FUNCTION: Bactericidal antibiotic. Active on bacteria phylogenetically
CC       related to the producing strain.
CC   -!- SUBCELLULAR LOCATION: Secreted. Host cell membrane {ECO:0000305};
CC       Single-pass membrane protein {ECO:0000305}. Note=Probably through the
CC       MchEF ABC transporter system.
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DR   EMBL; AJ009631; CAB54534.2; -; Genomic_DNA.
DR   RefSeq; WP_001375214.1; NZ_WTCP01000055.1.
DR   AlphaFoldDB; P62530; -.
DR   OMA; PANQIGA; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0020002; C:host cell plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0019835; P:cytolysis; IEA:UniProtKB-KW.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Antibiotic; Antimicrobial; Bacteriocin; Host cell membrane; Host membrane;
KW   Membrane; Secreted; Transmembrane; Transmembrane helix.
FT   PROPEP          1..15
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000021660"
FT   CHAIN           16..75
FT                   /note="Microcin H47"
FT                   /id="PRO_0000021661"
FT   TRANSMEM        30..50
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   75 AA;  6600 MW;  1AD5EE9F1B58123C CRC64;
     MREITESQLR YISGAGGAPA TSANAAGAAA IVGALAGIPG GPLGVVVGAV SAGLTTAIGS
     TVGSGSASSS AGGGS