ID   MCHE_ECOLX              Reviewed;         424 AA.
AC   Q9EXN6; Q2WEK6;
DT   26-SEP-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   25-MAY-2022, entry version 79.
DE   RecName: Full=Microcin H47 secretion protein MchE;
GN   Name=mchE;
OS   Escherichia coli.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=H47;
RX   PubMed=11181394; DOI=10.1128/aac.45.3.969-972.2001;
RA   Azpiroz M.F., Rodriguez E., Lavina M.;
RT   "The structure, function, and origin of the microcin H47 ATP-binding
RT   cassette exporter indicate its relatedness to that of colicin V.";
RL   Antimicrob. Agents Chemother. 45:969-972(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=H47;
RX   PubMed=15047525; DOI=10.1128/aac.48.4.1235-1241.2004;
RA   Azpiroz M.F., Lavina M.;
RT   "Involvement of enterobactin synthesis pathway in production of microcin
RT   H47.";
RL   Antimicrob. Agents Chemother. 48:1235-1241(2004).
CC   -!- FUNCTION: Probably involved, in conjunction with MchF, in the secretion
CC       of microcin H47.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000250}; Single-pass
CC       membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the membrane fusion protein (MFP) (TC 8.A.1)
CC       family. {ECO:0000305}.
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DR   EMBL; AJ009631; CAJ44958.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q9EXN6; -.
DR   SMR; Q9EXN6; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0043213; P:bacteriocin transport; IEA:UniProtKB-KW.
DR   GO; GO:0009306; P:protein secretion; IEA:InterPro.
DR   GO; GO:0055085; P:transmembrane transport; IEA:InterPro.
DR   InterPro; IPR043602; CusB_dom_1.
DR   InterPro; IPR032317; HlyD_D23.
DR   InterPro; IPR006144; Secretion_HlyD_CS.
DR   Pfam; PF00529; CusB_dom_1; 1.
DR   Pfam; PF16576; HlyD_D23; 1.
DR   PROSITE; PS00543; HLYD_FAMILY; 1.
PE   3: Inferred from homology;
KW   Bacteriocin transport; Cell inner membrane; Cell membrane; Membrane;
KW   Protein transport; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..424
FT                   /note="Microcin H47 secretion protein MchE"
FT                   /id="PRO_0000201881"
FT   TOPO_DOM        1..25
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        26..46
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        47..424
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   424 AA;  48720 MW;  3CAD07D9EC3A41F4 CRC64;
     MFRQDALENR KMKWQGRAIL LPGIPLWLIM LGSIVFITAF LMFIIVGTYS RRVNVSGEVT
     TWPRAVNIYS GVQGFVVRQF VHEGQLIKKG DPVYLIDISK STRSGIVTDN HRRDIENQLV
     RVDNIISRLE ESKKITLDTL EKQRLQYTDA FRRSSDIIQR AEEGIKIMKN NMENYRNYQA
     KGLINKDQLT NQVALYYQQQ NNLLSLSGQN EQNALQITTL ESQIQTQAAD FDNRIYQMEL
     QRYELQKELV NTDVEGEIII RALTDGKVDS LSVTVGQMVN TGDSLLQVIP ENIENYYLIL
     WVPNDAVPYI SAGDKVNIRY EAFPAEKFGQ FSATVKTISR TPASTQEMLT YKGAPQNTPG
     ASVPWYKVIA MPEKQIIRYD EKYLPLENGM KAESTLFLEK RRIYQWMLSP FYDMKHSATG
     PLND