ID   MCHI_ECOLX              Reviewed;          69 AA.
AC   O86200;
DT   26-SEP-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1998, sequence version 1.
DT   25-MAY-2022, entry version 66.
DE   RecName: Full=Microcin H47 immunity protein MchI;
GN   Name=mchI;
OS   Escherichia coli.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND CHARACTERIZATION.
RC   STRAIN=H47;
RX   PubMed=9783429; DOI=10.1139/w98-044;
RA   Rodriguez E., Lavina M.;
RT   "Genetic analysis of microcin H47 immunity.";
RL   Can. J. Microbiol. 44:692-697(1998).
CC   -!- FUNCTION: Protects a microcin H47-producer cell against microcin H47.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
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DR   EMBL; X97613; CAA66217.1; -; Genomic_DNA.
DR   EMBL; AJ009631; CAB54533.1; -; Genomic_DNA.
DR   RefSeq; WP_000120664.1; NZ_WSWV01000106.1.
DR   AlphaFoldDB; O86200; -.
DR   SMR; O86200; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0030153; P:bacteriocin immunity; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Bacteriocin immunity; Cell membrane; Membrane; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..69
FT                   /note="Microcin H47 immunity protein MchI"
FT                   /id="PRO_0000096278"
FT   TOPO_DOM        1..6
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        7..29
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        30..38
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        39..61
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        62..69
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   69 AA;  7807 MW;  FCF61A043AB1BEC9 CRC64;
     MSYKKLYQLT AIFSLPLTIL LVSLSSLRIV GEGNSYVDVF LSFIIFLGFI ELIHGIRKIL
     VWSGWKNGS