ID   MCH_CANLF               Reviewed;         165 AA.
AC   Q5XXR2;
DT   26-APR-2005, integrated into UniProtKB/Swiss-Prot.
DT   23-NOV-2004, sequence version 1.
DT   03-AUG-2022, entry version 91.
DE   RecName: Full=Pro-MCH;
DE   Contains:
DE     RecName: Full=Neuropeptide-glycine-glutamic acid;
DE              Short=NGE;
DE              Short=Neuropeptide G-E;
DE   Contains:
DE     RecName: Full=Neuropeptide-glutamic acid-isoleucine;
DE              Short=NEI;
DE              Short=Neuropeptide E-I;
DE   Contains:
DE     RecName: Full=Melanin-concentrating hormone;
DE              Short=MCH;
DE   Flags: Precursor;
GN   Name=PMCH; Synonyms=MCH;
OS   Canis lupus familiaris (Dog) (Canis familiaris).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Carnivora; Caniformia; Canidae; Canis.
OX   NCBI_TaxID=9615;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=Beagle; TISSUE=Hypothalamus;
RA   Steflik J., Waters S.M.;
RT   "Canine pro-melanin-concentrating hormone.";
RL   Submitted (AUG-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: MCH may act as a neurotransmitter or neuromodulator in a
CC       broad array of neuronal functions directed toward the regulation of
CC       goal-directed behavior, such as food intake, and general arousal.
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC   -!- PTM: Pro-MCH is processed differentially in the brain and in peripheral
CC       organs producing two neuropeptides; NEI and MCH. A third peptide, NGE,
CC       may also be produced. Preferential processing in neurons by prohormone
CC       convertase 2 (PC2) generates NEI. MCH is generated in neurons of the
CC       lateral hypothalmic area by several prohormone convertases including
CC       PC1/3, PC2 and PC5/6 (By similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the melanin-concentrating hormone family.
CC       {ECO:0000305}.
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DR   EMBL; AY725987; AAU43637.1; -; mRNA.
DR   RefSeq; NP_001005755.1; NM_001005755.1.
DR   AlphaFoldDB; Q5XXR2; -.
DR   STRING; 9615.ENSCAFP00000010824; -.
DR   PaxDb; Q5XXR2; -.
DR   Ensembl; ENSCAFT00030017902; ENSCAFP00030015634; ENSCAFG00030009674.
DR   Ensembl; ENSCAFT00040031414; ENSCAFP00040027319; ENSCAFG00040017015.
DR   Ensembl; ENSCAFT00845016275; ENSCAFP00845012661; ENSCAFG00845009246.
DR   GeneID; 449022; -.
DR   KEGG; cfa:449022; -.
DR   CTD; 5367; -.
DR   VEuPathDB; HostDB:ENSCAFG00845009246; -.
DR   eggNOG; ENOG502RZ12; Eukaryota.
DR   GeneTree; ENSGT00390000004984; -.
DR   HOGENOM; CLU_1610172_0_0_1; -.
DR   InParanoid; Q5XXR2; -.
DR   OMA; NTFRMGK; -.
DR   OrthoDB; 1451976at2759; -.
DR   TreeFam; TF330944; -.
DR   Reactome; R-CFA-375276; Peptide ligand-binding receptors.
DR   Reactome; R-CFA-416476; G alpha (q) signalling events.
DR   Reactome; R-CFA-418594; G alpha (i) signalling events.
DR   Proteomes; UP000002254; Chromosome 15.
DR   Bgee; ENSCAFG00000007297; Expressed in keratinocyte and 37 other tissues.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0005634; C:nucleus; IEA:Ensembl.
DR   GO; GO:0045202; C:synapse; IEA:GOC.
DR   GO; GO:0030354; F:melanin-concentrating hormone activity; IEA:InterPro.
DR   GO; GO:0031777; F:type 1 melanin-concentrating hormone receptor binding; IBA:GO_Central.
DR   GO; GO:0007268; P:chemical synaptic transmission; IEA:InterPro.
DR   GO; GO:0007631; P:feeding behavior; IEA:Ensembl.
DR   GO; GO:0007218; P:neuropeptide signaling pathway; IEA:UniProtKB-KW.
DR   InterPro; IPR005456; Prepro-melanin_conc_hormone.
DR   PANTHER; PTHR12091; PTHR12091; 1.
DR   Pfam; PF05824; Pro-MCH; 1.
DR   PRINTS; PR01641; PROMCHFAMILY.
PE   2: Evidence at transcript level;
KW   Amidation; Cleavage on pair of basic residues; Disulfide bond; Hormone;
KW   Neuropeptide; Reference proteome; Secreted; Signal.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000255"
FT   CHAIN           22..165
FT                   /note="Pro-MCH"
FT                   /id="PRO_0000019100"
FT   PEPTIDE         110..128
FT                   /note="Neuropeptide-glycine-glutamic acid"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000019101"
FT   PEPTIDE         131..143
FT                   /note="Neuropeptide-glutamic acid-isoleucine"
FT                   /id="PRO_0000019102"
FT   PEPTIDE         147..165
FT                   /note="Melanin-concentrating hormone"
FT                   /id="PRO_0000019103"
FT   REGION          68..88
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        70..84
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         143
FT                   /note="Isoleucine amide"
FT                   /evidence="ECO:0000250"
FT   DISULFID        153..162
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   165 AA;  18644 MW;  F34A776677F4044A CRC64;
     MAKMSLSSYL LILTFSLFSQ GILLSASKSI RNLEDDMVFN TFRLGKALQK EDTPQKSVVA
     PSLEQYKNDD SSFMNDEENK NSKNTGSKHN FLNHGLPLNL AIKPYLALKG SVAFPAENGV
     QNTESTQEKR EIGDEENSAK FPIGRRDFDM LRCMLGRVYR PCWQV