MCH_HALHT
ID MCH_HALHT Reviewed; 358 AA.
AC G0HQ35;
DT 10-FEB-2021, integrated into UniProtKB/Swiss-Prot.
DT 19-OCT-2011, sequence version 1.
DT 25-MAY-2022, entry version 43.
DE RecName: Full=Mesaconyl-CoA hydratase {ECO:0000303|PubMed:28932214};
DE EC=4.2.1.148;
GN Name=mch {ECO:0000303|PubMed:28932214};
GN OrderedLocusNames=HAH_1340 {ECO:0000312|EMBL:AEM56954.1};
OS Haloarcula hispanica (strain ATCC 33960 / DSM 4426 / JCM 8911 / NBRC 102182
OS / NCIMB 2187 / VKM B-1755).
OC Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Halobacteriales;
OC Haloarculaceae; Haloarcula.
OX NCBI_TaxID=634497;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 33960 / DSM 4426 / JCM 8911 / NBRC 102182 / NCIMB 2187 / VKM
RC B-1755;
RX PubMed=21994921; DOI=10.1128/jb.05953-11;
RA Liu H., Wu Z., Li M., Zhang F., Zheng H., Han J., Liu J., Zhou J., Wang S.,
RA Xiang H.;
RT "Complete genome sequence of Haloarcula hispanica, a model haloarchaeon for
RT studying genetics, metabolism, and virus-host interaction.";
RL J. Bacteriol. 193:6086-6087(2011).
RN [2]
RP FUNCTION, CATALYTIC ACTIVITY, ACTIVITY REGULATION, BIOPHYSICOCHEMICAL
RP PROPERTIES, AND SUBUNIT.
RX PubMed=28932214; DOI=10.3389/fmicb.2017.01683;
RA Borjian F., Johnsen U., Schoenheit P., Berg I.A.;
RT "Succinyl-CoA:mesaconate CoA-transferase and mesaconyl-CoA hydratase,
RT enzymes of the methylaspartate cycle in Haloarcula hispanica.";
RL Front. Microbiol. 8:1683-1683(2017).
CC -!- FUNCTION: Involved in the methylaspartate cycle. Catalyzes the
CC reversible hydration of mesaconyl-CoA (2-methylfumaryl-CoA) to yield
CC beta-methylmalyl-CoA ((2R,3S)-beta-methylmalyl-CoA) (PubMed:28932214).
CC Shows also activity with mesaconyl-C4-CoA (3-methylfumaryl-CoA), (S)-
CC citramalyl-CoA and (S)-malyl-CoA (PubMed:28932214).
CC {ECO:0000269|PubMed:28932214}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(2R,3S)-beta-methylmalyl-CoA = 2-methylfumaryl-CoA + H2O;
CC Xref=Rhea:RHEA:38263, ChEBI:CHEBI:15377, ChEBI:CHEBI:75634,
CC ChEBI:CHEBI:75635; EC=4.2.1.148;
CC Evidence={ECO:0000269|PubMed:28932214};
CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:38265;
CC Evidence={ECO:0000269|PubMed:28932214};
CC -!- ACTIVITY REGULATION: Shows highest activity at 0.5 M KCl. Does not
CC require divalent ions for activity. {ECO:0000269|PubMed:28932214}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=1.0 mM for mesaconyl-CoA {ECO:0000269|PubMed:28932214};
CC KM=0.35 mM for beta-methylmalyl-CoA {ECO:0000269|PubMed:28932214};
CC KM=0.18 mM for mesaconyl-C4-CoA {ECO:0000269|PubMed:28932214};
CC KM=2.6 mM for (S)-citramalyl-CoA {ECO:0000269|PubMed:28932214};
CC KM=0.32 mM for (S)-malyl-CoA {ECO:0000269|PubMed:28932214};
CC Vmax=20.7 umol/min/mg enzyme with mesaconyl-CoA as substrate
CC {ECO:0000269|PubMed:28932214};
CC Vmax=266 umol/min/mg enzyme with beta-methylmalyl-CoA as substrate
CC {ECO:0000269|PubMed:28932214};
CC Vmax=1.12 umol/min/mg enzyme with mesaconyl-C4-CoA as substrate
CC {ECO:0000269|PubMed:28932214};
CC Vmax=1.6 umol/min/mg enzyme with (S)-citramalyl-CoA as substrate
CC {ECO:0000269|PubMed:28932214};
CC Vmax=3.6 umol/min/mg enzyme with (S)-malyl-CoA as substrate
CC {ECO:0000269|PubMed:28932214};
CC -!- SUBUNIT: Homodimer. {ECO:0000269|PubMed:28932214}.
CC -!- SIMILARITY: Belongs to the enoyl-CoA hydratase/isomerase family.
CC {ECO:0000305}.
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DR EMBL; CP002921; AEM56954.1; -; Genomic_DNA.
DR RefSeq; WP_014040205.1; NC_015948.1.
DR AlphaFoldDB; G0HQ35; -.
DR SMR; G0HQ35; -.
DR STRING; 634497.HAH_1340; -.
DR EnsemblBacteria; AEM56954; AEM56954; HAH_1340.
DR GeneID; 23804720; -.
DR KEGG; hhi:HAH_1340; -.
DR eggNOG; arCOG00774; Archaea.
DR eggNOG; arCOG00775; Archaea.
DR HOGENOM; CLU_777564_0_0_2; -.
DR OMA; QTLNHDP; -.
DR OrthoDB; 32446at2157; -.
DR BRENDA; 4.2.1.148; 8340.
DR Proteomes; UP000005629; Chromosome I.
DR GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
DR InterPro; IPR029069; HotDog_dom_sf.
DR InterPro; IPR045672; MC_hydratase.
DR Pfam; PF19315; MC_hydratase; 1.
DR SUPFAM; SSF54637; SSF54637; 2.
PE 1: Evidence at protein level;
KW Lyase.
FT CHAIN 1..358
FT /note="Mesaconyl-CoA hydratase"
FT /id="PRO_0000451973"
SQ SEQUENCE 358 AA; 39909 MW; 4C6EED54E36054A0 CRC64;
MTDWTDPDAL DLSDGETFDS LLDRADTREK GHFFEFFAEG DELAHDPGLR LSHHGSEQWM
GQTLNHDPAY WRADTAQERG FEERPVHPDY LLACVMGITV EDLSEKGGYF LGRDDVEFHQ
PATAGTPLSV TSTVVDTRTS SSRPKYGIVT WETEGRDRET GETLVSYRRT NMIPRREPAA
TDGGAVGEQD EGGPAMPDTL LSPDGERFGD FQAALDTARE ENAAVAYRHE RGRTMDDQLV
AGLPLSTLNT ARQHHNRDEM ADSPSGDIVA YGDVTRSIAL AHARSDEATY REQRFADERF
HDFVTLGDTV YGFTRVLDCD PDAGPERAGA VTFEHVAFNQ EQTPVYSGRR TAHIQRHQ
