ID   MCH_METCA               Reviewed;         331 AA.
AC   Q9RPW8; Q603E4;
DT   16-NOV-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   25-MAY-2022, entry version 108.
DE   RecName: Full=Methenyltetrahydromethanopterin cyclohydrolase;
DE            EC=3.5.4.27;
DE   AltName: Full=Methenyl-H4MPT cyclohydrolase;
GN   Name=mch; OrderedLocusNames=MCA2863;
OS   Methylococcus capsulatus (strain ATCC 33009 / NCIMB 11132 / Bath).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Methylococcales;
OC   Methylococcaceae; Methylococcus.
OX   NCBI_TaxID=243233;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 33009 / NCIMB 11132 / Bath;
RX   PubMed=10482517; DOI=10.1128/jb.181.18.5750-5757.1999;
RA   Vorholt J.A., Chistoserdova L.V., Stolyar S.M., Thauer R.K., Lidstrom M.E.;
RT   "Distribution of tetrahydromethanopterin-dependent enzymes in
RT   methylotrophic bacteria and phylogeny of methenyl tetrahydromethanopterin
RT   cyclohydrolases.";
RL   J. Bacteriol. 181:5750-5757(1999).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 33009 / NCIMB 11132 / Bath;
RX   PubMed=15383840; DOI=10.1371/journal.pbio.0020303;
RA   Ward N.L., Larsen O., Sakwa J., Bruseth L., Khouri H.M., Durkin A.S.,
RA   Dimitrov G., Jiang L., Scanlan D., Kang K.H., Lewis M.R., Nelson K.E.,
RA   Methe B.A., Wu M., Heidelberg J.F., Paulsen I.T., Fouts D.E., Ravel J.,
RA   Tettelin H., Ren Q., Read T.D., DeBoy R.T., Seshadri R., Salzberg S.L.,
RA   Jensen H.B., Birkeland N.K., Nelson W.C., Dodson R.J., Grindhaug S.H.,
RA   Holt I.E., Eidhammer I., Jonasen I., Vanaken S., Utterback T.R.,
RA   Feldblyum T.V., Fraser C.M., Lillehaug J.R., Eisen J.A.;
RT   "Genomic insights into methanotrophy: the complete genome sequence of
RT   Methylococcus capsulatus (Bath).";
RL   PLoS Biol. 2:1616-1628(2004).
CC   -!- FUNCTION: Catalyzes the hydrolysis of methenyl-H(4)MPT(+) to 5-formyl-
CC       H(4)MPT. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O = H(+) +
CC         N(5)-formyl-5,6,7,8-tetrahydromethanopterin; Xref=Rhea:RHEA:19053,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:58018,
CC         ChEBI:CHEBI:58337; EC=3.5.4.27;
CC   -!- PATHWAY: One-carbon metabolism; formaldehyde degradation; formate from
CC       formaldehyde (H(4)MPT route): step 3/5.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the MCH family. {ECO:0000305}.
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DR   EMBL; AF142649; AAD55899.1; -; Genomic_DNA.
DR   EMBL; AE017282; AAU91100.1; -; Genomic_DNA.
DR   RefSeq; WP_010962058.1; NC_002977.6.
DR   AlphaFoldDB; Q9RPW8; -.
DR   SMR; Q9RPW8; -.
DR   STRING; 243233.MCA2863; -.
DR   EnsemblBacteria; AAU91100; AAU91100; MCA2863.
DR   KEGG; mca:MCA2863; -.
DR   eggNOG; COG3252; Bacteria.
DR   HOGENOM; CLU_876031_0_0_6; -.
DR   OMA; DKGMFAP; -.
DR   OrthoDB; 1691428at2; -.
DR   BRENDA; 3.5.4.27; 3305.
DR   UniPathway; UPA00562; UER00703.
DR   Proteomes; UP000006821; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0018759; F:methenyltetrahydromethanopterin cyclohydrolase activity; ISS:JCVI.
DR   GO; GO:0046294; P:formaldehyde catabolic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0015947; P:methane metabolic process; ISS:JCVI.
DR   GO; GO:0006730; P:one-carbon metabolic process; IEA:UniProtKB-UniRule.
DR   CDD; cd00545; MCH; 1.
DR   HAMAP; MF_00486; McH; 1.
DR   InterPro; IPR003209; METHMP_CycHdrlase.
DR   Pfam; PF02289; MCH; 1.
DR   TIGRFAMs; TIGR03120; one_C_mch; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Hydrolase; One-carbon metabolism; Reference proteome.
FT   CHAIN           1..331
FT                   /note="Methenyltetrahydromethanopterin cyclohydrolase"
FT                   /id="PRO_0000140888"
SQ   SEQUENCE   331 AA;  35897 MW;  72B219414FDC0D38 CRC64;
     MQNRVSVNAH SQPIVKYLIT YADKLRLQID RLPNGCTVID AGIKVPGSLE AGRLIGEICM
     GGLGRITLTH TDTFTKWPLT VNVHTSNPVI ACLGSQYAGW SLSHGEGKDG FYALGSGPAR
     AMATKIKDGN EEPVEELYKE LNYRDKHTET AIVMEVDKVP PVEVTDKIAR ACKLDPSHLT
     VILTPTSSLA GGMQVVARVL EVALHKAHAL HFPLENIIDG TGCAPVPPPH PNFIKAMGRT
     NDAILFGGRV HLFVKGSDEA AETLANELPS SVSRDYGKPF AEVFKEYKYD FFKVDAMLFS
     PASVIVTTVD SGRSFHAGEV DVELLERSFG G