ID   MCH_METEA               Reviewed;         323 AA.
AC   O85014; C5B142;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1998, sequence version 1.
DT   03-AUG-2022, entry version 125.
DE   RecName: Full=Methenyltetrahydromethanopterin cyclohydrolase;
DE            EC=3.5.4.27;
DE   AltName: Full=Methenyl-H4MPT cyclohydrolase;
GN   Name=mch; OrderedLocusNames=MexAM1_META1p1763;
OS   Methylorubrum extorquens (strain ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB
OS   9133 / AM1) (Methylobacterium extorquens).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Methylobacteriaceae; Methylorubrum.
OX   NCBI_TaxID=272630;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=9651254; DOI=10.1126/science.281.5373.99;
RA   Chistoserdova L.V., Vorholt J.A., Thauer R.K., Lidstrom M.E.;
RT   "C1 transfer enzymes and coenzymes linking methylotrophic bacteria and
RT   methanogenic Archaea.";
RL   Science 281:99-102(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1;
RX   PubMed=19440302; DOI=10.1371/journal.pone.0005584;
RA   Vuilleumier S., Chistoserdova L., Lee M.-C., Bringel F., Lajus A., Zhou Y.,
RA   Gourion B., Barbe V., Chang J., Cruveiller S., Dossat C., Gillett W.,
RA   Gruffaz C., Haugen E., Hourcade E., Levy R., Mangenot S., Muller E.,
RA   Nadalig T., Pagni M., Penny C., Peyraud R., Robinson D.G., Roche D.,
RA   Rouy Z., Saenampechek C., Salvignol G., Vallenet D., Wu Z., Marx C.J.,
RA   Vorholt J.A., Olson M.V., Kaul R., Weissenbach J., Medigue C.,
RA   Lidstrom M.E.;
RT   "Methylobacterium genome sequences: a reference blueprint to investigate
RT   microbial metabolism of C1 compounds from natural and industrial sources.";
RL   PLoS ONE 4:E5584-E5584(2009).
RN   [3]
RP   CHARACTERIZATION, AND PROTEIN SEQUENCE OF 1-15.
RX   PubMed=10215859; DOI=10.1046/j.1432-1327.1999.00291.x;
RA   Pomper B.K., Vorholt J.A., Chistoserdova L.V., Lidstrom M.E., Thauer R.K.;
RT   "A methenyl tetrahydromethanopterin cyclohydrolase and a methenyl
RT   tetrahydrofolate cyclohydrolase in Methylobacterium extorquens AM1.";
RL   Eur. J. Biochem. 261:475-480(1999).
CC   -!- FUNCTION: Catalyzes the hydrolysis of methenyl-H(4)MPT(+) to 5-formyl-
CC       H(4)MPT.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O = H(+) +
CC         N(5)-formyl-5,6,7,8-tetrahydromethanopterin; Xref=Rhea:RHEA:19053,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:58018,
CC         ChEBI:CHEBI:58337; EC=3.5.4.27;
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       pH dependence:
CC         Optimum pH is 8.5.;
CC       Temperature dependence:
CC         Optimum temperature is 40 degrees Celsius.;
CC   -!- PATHWAY: One-carbon metabolism; formaldehyde degradation; formate from
CC       formaldehyde (H(4)MPT route): step 3/5.
CC   -!- SUBUNIT: Homodimer.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- MISCELLANEOUS: Requires salt for optimal activity.
CC   -!- SIMILARITY: Belongs to the MCH family. {ECO:0000305}.
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DR   EMBL; AF032114; AAC27022.1; -; Genomic_DNA.
DR   EMBL; CP001510; ACS39606.1; -; Genomic_DNA.
DR   RefSeq; WP_003597572.1; NC_012808.1.
DR   AlphaFoldDB; O85014; -.
DR   SMR; O85014; -.
DR   STRING; 272630.MexAM1_META1p1763; -.
DR   EnsemblBacteria; ACS39606; ACS39606; MexAM1_META1p1763.
DR   KEGG; mea:Mex_1p1763; -.
DR   eggNOG; COG3252; Bacteria.
DR   HOGENOM; CLU_876031_0_0_5; -.
DR   OMA; DKGMFAP; -.
DR   OrthoDB; 1691428at2; -.
DR   BioCyc; MetaCyc:MON-4021; -.
DR   UniPathway; UPA00562; UER00703.
DR   Proteomes; UP000009081; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0018759; F:methenyltetrahydromethanopterin cyclohydrolase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0046294; P:formaldehyde catabolic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0006730; P:one-carbon metabolic process; IEA:UniProtKB-UniRule.
DR   CDD; cd00545; MCH; 1.
DR   HAMAP; MF_00486; McH; 1.
DR   InterPro; IPR003209; METHMP_CycHdrlase.
DR   Pfam; PF02289; MCH; 1.
DR   TIGRFAMs; TIGR03120; one_C_mch; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; Direct protein sequencing; Hydrolase; One-carbon metabolism.
FT   CHAIN           1..323
FT                   /note="Methenyltetrahydromethanopterin cyclohydrolase"
FT                   /id="PRO_0000140889"
SQ   SEQUENCE   323 AA;  33283 MW;  30A4E9978E2F5521 CRC64;
     MSSNTSAPSL NALAGPLVES LVADAAKLRL IVAQENGART VDAGANARGS IEAGRRIAEI
     CLGGLGTVTI APIGPVASWP YTVVVHSADP VLACLGSQYA GWSLADEEGD SGFFALGSGP
     GRAVAVVEEL YKELGYRDNA TTTALVLESG SAPPASVVNK VAAATGLAPE NVTFIYAPTQ
     SLAGSTQVVA RVLEVALHKA HTVGFDLHKI LDGIGSAPLS PPHPDFIQAM GRTNDAIIYG
     GRVQLFVDAD DADAKQLAEQ IPSTTSADHG APFAEIFSRV NGDFYKIDGA LFSPAEAIVT
     SVKTGKSFRG GRLEPQLVDA SFV