MCH_METED
ID MCH_METED Reviewed; 323 AA.
AC Q8KMJ5; C7CCN5;
DT 21-NOV-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 25-MAY-2022, entry version 91.
DE RecName: Full=Methenyltetrahydromethanopterin cyclohydrolase;
DE EC=3.5.4.27;
DE AltName: Full=Methenyl-H4MPT cyclohydrolase;
GN Name=mch; Synonyms=mchA; OrderedLocusNames=METDI2515;
OS Methylorubrum extorquens (strain DSM 6343 / CIP 106787 / DM4)
OS (Methylobacterium extorquens).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Methylobacteriaceae; Methylorubrum.
OX NCBI_TaxID=661410;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=12055310; DOI=10.1099/00221287-148-6-1915;
RA Kayser M.F., Ucurum Z., Vuilleumier S.;
RT "Dichloromethane metabolism and C1 utilization genes in Methylobacterium
RT strains.";
RL Microbiology 148:1915-1922(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 6343 / CIP 106787 / DM4;
RX PubMed=19440302; DOI=10.1371/journal.pone.0005584;
RA Vuilleumier S., Chistoserdova L., Lee M.-C., Bringel F., Lajus A., Zhou Y.,
RA Gourion B., Barbe V., Chang J., Cruveiller S., Dossat C., Gillett W.,
RA Gruffaz C., Haugen E., Hourcade E., Levy R., Mangenot S., Muller E.,
RA Nadalig T., Pagni M., Penny C., Peyraud R., Robinson D.G., Roche D.,
RA Rouy Z., Saenampechek C., Salvignol G., Vallenet D., Wu Z., Marx C.J.,
RA Vorholt J.A., Olson M.V., Kaul R., Weissenbach J., Medigue C.,
RA Lidstrom M.E.;
RT "Methylobacterium genome sequences: a reference blueprint to investigate
RT microbial metabolism of C1 compounds from natural and industrial sources.";
RL PLoS ONE 4:E5584-E5584(2009).
CC -!- FUNCTION: Catalyzes the hydrolysis of methenyl-H(4)MPT(+) to 5-formyl-
CC H(4)MPT. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O = H(+) +
CC N(5)-formyl-5,6,7,8-tetrahydromethanopterin; Xref=Rhea:RHEA:19053,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:58018,
CC ChEBI:CHEBI:58337; EC=3.5.4.27;
CC -!- PATHWAY: One-carbon metabolism; formaldehyde degradation; formate from
CC formaldehyde (H(4)MPT route): step 3/5.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the MCH family. {ECO:0000305}.
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DR EMBL; AJ421477; CAD13315.1; -; Genomic_DNA.
DR EMBL; FP103042; CAX24170.1; -; Genomic_DNA.
DR AlphaFoldDB; Q8KMJ5; -.
DR SMR; Q8KMJ5; -.
DR EnsemblBacteria; CAX24170; CAX24170; METD_I2515.
DR KEGG; mdi:METDI2515; -.
DR HOGENOM; CLU_876031_0_0_5; -.
DR OMA; DKGMFAP; -.
DR UniPathway; UPA00562; UER00703.
DR Proteomes; UP000008070; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0018759; F:methenyltetrahydromethanopterin cyclohydrolase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0046294; P:formaldehyde catabolic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0006730; P:one-carbon metabolic process; IEA:UniProtKB-UniRule.
DR CDD; cd00545; MCH; 1.
DR HAMAP; MF_00486; McH; 1.
DR InterPro; IPR003209; METHMP_CycHdrlase.
DR Pfam; PF02289; MCH; 1.
DR TIGRFAMs; TIGR03120; one_C_mch; 1.
PE 3: Inferred from homology;
KW Cytoplasm; Hydrolase; One-carbon metabolism.
FT CHAIN 1..323
FT /note="Methenyltetrahydromethanopterin cyclohydrolase"
FT /id="PRO_0000140894"
SQ SEQUENCE 323 AA; 33268 MW; 900FF2379E84F531 CRC64;
MSSNTSAPSL NALAGPLVES LVADAAKLRL IVAQENGART VDAGANARGS IEAGRRIAEI
CLGGLGTVTI APIGPVASWP YTVVVHSADP VLACLGSQYA GWSLADEDGD SGFFALGSGP
GRAVAVVEEL YKELGYRDNA TTTALVLESG SAPPASVVNK VAAATGLAPE NVTFIYAPTQ
SLAGSTQVVA RVLEVALHKA HTVGFDLHKI LDGIGSAPLS PPHPDFIQAM GRTNDAIIYG
GRVQLFVDAD DADAKQLAEQ IPSTTSADHG APFAEIFSRV NGDFYKIDGA LFSPAEAIVT
SVKTGKSFRG GRLEPQLVDA SFV
