ID   MCH_METFK               Reviewed;         327 AA.
AC   Q9RQ04; Q1H0R1;
DT   16-NOV-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   25-MAY-2022, entry version 103.
DE   RecName: Full=Methenyltetrahydromethanopterin cyclohydrolase;
DE            EC=3.5.4.27;
DE   AltName: Full=Methenyl-H4MPT cyclohydrolase;
GN   Name=mch; OrderedLocusNames=Mfla_1658;
OS   Methylobacillus flagellatus (strain KT / ATCC 51484 / DSM 6875).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
OC   Methylophilaceae; Methylobacillus.
OX   NCBI_TaxID=265072;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=10482517; DOI=10.1128/jb.181.18.5750-5757.1999;
RA   Vorholt J.A., Chistoserdova L.V., Stolyar S.M., Thauer R.K., Lidstrom M.E.;
RT   "Distribution of tetrahydromethanopterin-dependent enzymes in
RT   methylotrophic bacteria and phylogeny of methenyl tetrahydromethanopterin
RT   cyclohydrolases.";
RL   J. Bacteriol. 181:5750-5757(1999).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=KT / ATCC 51484 / DSM 6875;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S.,
RA   Brettin T., Bruce D., Han C., Tapia R., Saunders E., Gilna P., Schmutz J.,
RA   Larimer F., Land M., Kyrpides N., Anderson I., Richardson P.;
RT   "Complete sequence of Methylobacillus flagellatus KT.";
RL   Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the hydrolysis of methenyl-H(4)MPT(+) to 5-formyl-
CC       H(4)MPT. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O = H(+) +
CC         N(5)-formyl-5,6,7,8-tetrahydromethanopterin; Xref=Rhea:RHEA:19053,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:58018,
CC         ChEBI:CHEBI:58337; EC=3.5.4.27;
CC   -!- PATHWAY: One-carbon metabolism; formaldehyde degradation; formate from
CC       formaldehyde (H(4)MPT route): step 3/5.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the MCH family. {ECO:0000305}.
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DR   EMBL; AF139592; AAD55893.1; -; Genomic_DNA.
DR   EMBL; CP000284; ABE49926.1; -; Genomic_DNA.
DR   RefSeq; WP_011479880.1; NC_007947.1.
DR   AlphaFoldDB; Q9RQ04; -.
DR   SMR; Q9RQ04; -.
DR   STRING; 265072.Mfla_1658; -.
DR   EnsemblBacteria; ABE49926; ABE49926; Mfla_1658.
DR   KEGG; mfa:Mfla_1658; -.
DR   eggNOG; COG3252; Bacteria.
DR   HOGENOM; CLU_876031_0_0_4; -.
DR   OMA; DKGMFAP; -.
DR   OrthoDB; 1691428at2; -.
DR   UniPathway; UPA00562; UER00703.
DR   Proteomes; UP000002440; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0018759; F:methenyltetrahydromethanopterin cyclohydrolase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0046294; P:formaldehyde catabolic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0006730; P:one-carbon metabolic process; IEA:UniProtKB-UniRule.
DR   CDD; cd00545; MCH; 1.
DR   HAMAP; MF_00486; McH; 1.
DR   InterPro; IPR003209; METHMP_CycHdrlase.
DR   Pfam; PF02289; MCH; 1.
DR   TIGRFAMs; TIGR03120; one_C_mch; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Hydrolase; One-carbon metabolism; Reference proteome.
FT   CHAIN           1..327
FT                   /note="Methenyltetrahydromethanopterin cyclohydrolase"
FT                   /id="PRO_0000140890"
SQ   SEQUENCE   327 AA;  34910 MW;  6C3793ED34F1EBB7 CRC64;
     MSESKLDVTL WPSVNQLTNP LVKHLVDNAK ALRLLVEKLP SGATIIDAGI KAEGGLEAGR
     LIAEICMGGL GHVNLHSSST FPHWPWTLSV HSNNPVLSCL GSQYAGWSLS HEKFFSLGSG
     PGRALAGREE LYKELGYKDS ADAAVLVLES DKVPPQEVIE KVARDTGVNP ENLTFILTPT
     RSLAGTVQIV ARVLEVALHK IHTLHFPLEH VVDGAASAPL PPPAPDFLIG MGRTNDAILF
     GGHAHIFVKG SDEAAAKLAK ELPSSASRDY GRPFAEVFKA VNMDFYKIDP MLFSPAAVTV
     TAVESGKSFI GGKLDATLLD QSFGYSA