MCH_METHJ
ID MCH_METHJ Reviewed; 315 AA.
AC Q2FR24;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 21-MAR-2006, sequence version 1.
DT 25-MAY-2022, entry version 82.
DE RecName: Full=Methenyltetrahydromethanopterin cyclohydrolase {ECO:0000255|HAMAP-Rule:MF_00486};
DE EC=3.5.4.27 {ECO:0000255|HAMAP-Rule:MF_00486};
DE AltName: Full=Methenyl-H4MPT cyclohydrolase {ECO:0000255|HAMAP-Rule:MF_00486};
GN Name=mch {ECO:0000255|HAMAP-Rule:MF_00486}; OrderedLocusNames=Mhun_0444;
OS Methanospirillum hungatei JF-1 (strain ATCC 27890 / DSM 864 / NBRC 100397 /
OS JF-1).
OC Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC Methanomicrobiales; Methanospirillaceae; Methanospirillum.
OX NCBI_TaxID=323259;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 27890 / DSM 864 / NBRC 100397 / JF-1;
RX PubMed=26744606; DOI=10.1186/s40793-015-0124-8;
RA Gunsalus R.P., Cook L.E., Crable B., Rohlin L., McDonald E., Mouttaki H.,
RA Sieber J.R., Poweleit N., Zhou H., Lapidus A.L., Daligault H.E., Land M.,
RA Gilna P., Ivanova N., Kyrpides N., Culley D.E., McInerney M.J.;
RT "Complete genome sequence of Methanospirillum hungatei type strain JF1.";
RL Stand. Genomic Sci. 11:2-2(2016).
CC -!- FUNCTION: Catalyzes the reversible interconversion of 5-formyl-H(4)MPT
CC to methenyl-H(4)MPT(+). {ECO:0000255|HAMAP-Rule:MF_00486}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O = H(+) +
CC N(5)-formyl-5,6,7,8-tetrahydromethanopterin; Xref=Rhea:RHEA:19053,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:58018,
CC ChEBI:CHEBI:58337; EC=3.5.4.27; Evidence={ECO:0000255|HAMAP-
CC Rule:MF_00486};
CC -!- PATHWAY: One-carbon metabolism; methanogenesis from CO(2); 5,10-
CC methenyl-5,6,7,8-tetrahydromethanopterin from CO(2): step 3/3.
CC {ECO:0000255|HAMAP-Rule:MF_00486}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00486}.
CC -!- SIMILARITY: Belongs to the MCH family. {ECO:0000255|HAMAP-
CC Rule:MF_00486}.
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DR EMBL; CP000254; ABD40206.1; -; Genomic_DNA.
DR RefSeq; WP_011447494.1; NC_007796.1.
DR AlphaFoldDB; Q2FR24; -.
DR SMR; Q2FR24; -.
DR STRING; 323259.Mhun_0444; -.
DR EnsemblBacteria; ABD40206; ABD40206; Mhun_0444.
DR GeneID; 3925006; -.
DR KEGG; mhu:Mhun_0444; -.
DR eggNOG; arCOG02675; Archaea.
DR HOGENOM; CLU_876031_0_0_2; -.
DR OMA; DKGMFAP; -.
DR OrthoDB; 31274at2157; -.
DR UniPathway; UPA00640; UER00694.
DR Proteomes; UP000001941; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0018759; F:methenyltetrahydromethanopterin cyclohydrolase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0019386; P:methanogenesis, from carbon dioxide; IEA:UniProtKB-UniPathway.
DR GO; GO:0006730; P:one-carbon metabolic process; IEA:UniProtKB-UniRule.
DR CDD; cd00545; MCH; 1.
DR HAMAP; MF_00486; McH; 1.
DR InterPro; IPR003209; METHMP_CycHdrlase.
DR Pfam; PF02289; MCH; 1.
DR TIGRFAMs; TIGR03120; one_C_mch; 1.
PE 3: Inferred from homology;
KW Cytoplasm; Hydrolase; Methanogenesis; One-carbon metabolism;
KW Reference proteome.
FT CHAIN 1..315
FT /note="Methenyltetrahydromethanopterin cyclohydrolase"
FT /id="PRO_1000014398"
SQ SEQUENCE 315 AA; 33984 MW; D43F86E187FB26E9 CRC64;
MISVNELALE IFDNLANYSE DFNVAYHELD NGAKIVDCGV SVPGGYAAGR AFTEICMGGL
GEVNFRMGQI KHVPMPFIEV STDYPAISCL GAQKAGWTVK AGNYFAMGSG PARALALKPK
HTYEVIGYED ESDDAVIALE SDHLPNGEVM EKIAKDCGID VENLCAVVAP TASLVGSIQV
CGRCVETAVY KLNELGFDTR KITAAMGTAP IPPVKADATK AMGTTNDATI YHGQISLTMK
APDIVDFLEK IPSNKSKGYG KPFYEIFKEA NFDFYQIDTS LFSPAEVTIN ELTEGKVYHV
GEVNPDVTLK SFGYL
