MCH_METKA
ID MCH_METKA Reviewed; 316 AA.
AC P94954;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 4.
DT 25-MAY-2022, entry version 134.
DE RecName: Full=Methenyltetrahydromethanopterin cyclohydrolase;
DE EC=3.5.4.27;
DE AltName: Full=Methenyl-H4MPT cyclohydrolase;
GN Name=mch; OrderedLocusNames=MK0625;
OS Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938).
OC Archaea; Euryarchaeota; Methanopyri; Methanopyrales; Methanopyraceae;
OC Methanopyrus.
OX NCBI_TaxID=190192;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND CHARACTERIZATION.
RC STRAIN=AV19 / DSM 6324 / JCM 9639 / NBRC 100938;
RX PubMed=9676239; DOI=10.1007/s007920050038;
RA Vaupel M., Vorholt J.A., Thauer R.K.;
RT "Overproduction and one-step purification of the N5,N10-
RT methenyltetrahydromethanopterin cyclohydrolase (Mch) from the
RT hyperthermophilic Methanopyrus kandleri.";
RL Extremophiles 2:15-22(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AV19 / DSM 6324 / JCM 9639 / NBRC 100938;
RX PubMed=11930014; DOI=10.1073/pnas.032671499;
RA Slesarev A.I., Mezhevaya K.V., Makarova K.S., Polushin N.N.,
RA Shcherbinina O.V., Shakhova V.V., Belova G.I., Aravind L., Natale D.A.,
RA Rogozin I.B., Tatusov R.L., Wolf Y.I., Stetter K.O., Malykh A.G.,
RA Koonin E.V., Kozyavkin S.A.;
RT "The complete genome of hyperthermophile Methanopyrus kandleri AV19 and
RT monophyly of archaeal methanogens.";
RL Proc. Natl. Acad. Sci. U.S.A. 99:4644-4649(2002).
RN [3]
RP X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).
RC STRAIN=AV19 / DSM 6324 / JCM 9639 / NBRC 100938;
RX PubMed=10545331; DOI=10.1016/s0969-2126(00)80059-3;
RA Grabarse W., Vaupel M., Vorholt J.A., Shima S., Thauer R.K.,
RA Wittershagen A., Bourenkov G., Bartunik H.D., Ermler U.;
RT "The crystal structure of methenyltetrahydromethanopterin cyclohydrolase
RT from the hyperthermophilic archaeon Methanopyrus kandleri.";
RL Structure 7:1257-1268(1999).
CC -!- FUNCTION: Catalyzes the reversible interconversion of 5-formyl-H(4)MPT
CC to methenyl-H(4)MPT(+).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O = H(+) +
CC N(5)-formyl-5,6,7,8-tetrahydromethanopterin; Xref=Rhea:RHEA:19053,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:58018,
CC ChEBI:CHEBI:58337; EC=3.5.4.27;
CC -!- PATHWAY: One-carbon metabolism; methanogenesis from CO(2); 5,10-
CC methenyl-5,6,7,8-tetrahydromethanopterin from CO(2): step 3/3.
CC -!- SUBUNIT: Homotrimer.
CC -!- SUBCELLULAR LOCATION: Cytoplasm.
CC -!- SIMILARITY: Belongs to the MCH family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAM01840.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=CAA70072.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; Y08849; CAA70072.1; ALT_INIT; Genomic_DNA.
DR EMBL; AE009439; AAM01840.1; ALT_INIT; Genomic_DNA.
DR PIR; T45099; T45099.
DR PDB; 1QLM; X-ray; 2.00 A; A=1-316.
DR PDBsum; 1QLM; -.
DR AlphaFoldDB; P94954; -.
DR SMR; P94954; -.
DR STRING; 190192.MK0625; -.
DR EnsemblBacteria; AAM01840; AAM01840; MK0625.
DR KEGG; mka:MK0625; -.
DR PATRIC; fig|190192.8.peg.663; -.
DR HOGENOM; CLU_876031_0_0_2; -.
DR OMA; DKGMFAP; -.
DR UniPathway; UPA00640; UER00694.
DR EvolutionaryTrace; P94954; -.
DR Proteomes; UP000001826; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0018759; F:methenyltetrahydromethanopterin cyclohydrolase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0019386; P:methanogenesis, from carbon dioxide; IEA:UniProtKB-UniPathway.
DR GO; GO:0006730; P:one-carbon metabolic process; IEA:UniProtKB-UniRule.
DR CDD; cd00545; MCH; 1.
DR HAMAP; MF_00486; McH; 1.
DR InterPro; IPR003209; METHMP_CycHdrlase.
DR Pfam; PF02289; MCH; 1.
DR TIGRFAMs; TIGR03120; one_C_mch; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasm; Hydrolase; Methanogenesis; One-carbon metabolism;
KW Reference proteome.
FT INIT_MET 1
FT /note="Removed"
FT CHAIN 2..316
FT /note="Methenyltetrahydromethanopterin cyclohydrolase"
FT /id="PRO_0000140882"
FT HELIX 4..17
FT /evidence="ECO:0007829|PDB:1QLM"
FT HELIX 19..22
FT /evidence="ECO:0007829|PDB:1QLM"
FT STRAND 25..28
FT /evidence="ECO:0007829|PDB:1QLM"
FT STRAND 34..42
FT /evidence="ECO:0007829|PDB:1QLM"
FT HELIX 46..56
FT /evidence="ECO:0007829|PDB:1QLM"
FT TURN 57..59
FT /evidence="ECO:0007829|PDB:1QLM"
FT STRAND 61..70
FT /evidence="ECO:0007829|PDB:1QLM"
FT STRAND 73..84
FT /evidence="ECO:0007829|PDB:1QLM"
FT HELIX 85..89
FT /evidence="ECO:0007829|PDB:1QLM"
FT TURN 90..93
FT /evidence="ECO:0007829|PDB:1QLM"
FT STRAND 97..101
FT /evidence="ECO:0007829|PDB:1QLM"
FT STRAND 104..110
FT /evidence="ECO:0007829|PDB:1QLM"
FT HELIX 113..116
FT /evidence="ECO:0007829|PDB:1QLM"
FT HELIX 120..126
FT /evidence="ECO:0007829|PDB:1QLM"
FT STRAND 135..140
FT /evidence="ECO:0007829|PDB:1QLM"
FT HELIX 147..157
FT /evidence="ECO:0007829|PDB:1QLM"
FT HELIX 161..163
FT /evidence="ECO:0007829|PDB:1QLM"
FT STRAND 164..169
FT /evidence="ECO:0007829|PDB:1QLM"
FT STRAND 171..173
FT /evidence="ECO:0007829|PDB:1QLM"
FT HELIX 174..181
FT /evidence="ECO:0007829|PDB:1QLM"
FT HELIX 184..194
FT /evidence="ECO:0007829|PDB:1QLM"
FT HELIX 200..202
FT /evidence="ECO:0007829|PDB:1QLM"
FT STRAND 203..211
FT /evidence="ECO:0007829|PDB:1QLM"
FT HELIX 219..233
FT /evidence="ECO:0007829|PDB:1QLM"
FT STRAND 235..240
FT /evidence="ECO:0007829|PDB:1QLM"
FT HELIX 246..252
FT /evidence="ECO:0007829|PDB:1QLM"
FT HELIX 255..257
FT /evidence="ECO:0007829|PDB:1QLM"
FT TURN 259..262
FT /evidence="ECO:0007829|PDB:1QLM"
FT HELIX 265..271
FT /evidence="ECO:0007829|PDB:1QLM"
FT TURN 272..274
FT /evidence="ECO:0007829|PDB:1QLM"
FT HELIX 276..278
FT /evidence="ECO:0007829|PDB:1QLM"
FT HELIX 281..283
FT /evidence="ECO:0007829|PDB:1QLM"
FT STRAND 287..293
FT /evidence="ECO:0007829|PDB:1QLM"
FT TURN 294..296
FT /evidence="ECO:0007829|PDB:1QLM"
FT STRAND 299..303
FT /evidence="ECO:0007829|PDB:1QLM"
FT HELIX 307..313
FT /evidence="ECO:0007829|PDB:1QLM"
SQ SEQUENCE 316 AA; 34044 MW; D6D4097DE6BE0500 CRC64;
MVSVNENALP LVERMIERAE LLNVEVQELE NGTTVIDCGV EAAGGFEAGL LFSEVCMGGL
ATVELTEFEH DGLCLPAVQV TTDHPAVSTL AAQKAGWQVQ VGDYFAMGSG PARALALKPK
ETYEEIDYED DADVAILCLE SSELPDEDVA EHVADECGVD PENLYLLVAP TASIVGSVQV
SARVVETGLY KLLEVLEYDV TRVKYATGTA PIAPVADDDG EAMGRTNDCI LYGGTVYLYV
EGDDELPEVV EELPSEASED YGKPFMKIFE EADYDFYKID PGVFAPARVV VNDLSTGKTY
TAGEINVDVL KESFGL
