ID   MCH_METNO               Reviewed;         324 AA.
AC   B8ITX3;
DT   28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   03-MAR-2009, sequence version 1.
DT   25-MAY-2022, entry version 63.
DE   RecName: Full=Methenyltetrahydromethanopterin cyclohydrolase {ECO:0000255|HAMAP-Rule:MF_00486};
DE            EC=3.5.4.27 {ECO:0000255|HAMAP-Rule:MF_00486};
DE   AltName: Full=Methenyl-H4MPT cyclohydrolase {ECO:0000255|HAMAP-Rule:MF_00486};
GN   Name=mch {ECO:0000255|HAMAP-Rule:MF_00486}; OrderedLocusNames=Mnod_6004;
OS   Methylobacterium nodulans (strain LMG 21967 / CNCM I-2342 / ORS 2060).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Methylobacteriaceae; Methylobacterium.
OX   NCBI_TaxID=460265;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=LMG 21967 / CNCM I-2342 / ORS 2060;
RG   US DOE Joint Genome Institute;
RA   Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H.,
RA   Bruce D., Goodwin L., Pitluck S., Sims D., Brettin T., Detter J.C., Han C.,
RA   Larimer F., Land M., Hauser L., Kyrpides N., Ivanova N., Marx C.J.,
RA   Richardson P.;
RT   "Complete sequence of chromosome of Methylobacterium nodulans ORS 2060.";
RL   Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the hydrolysis of methenyl-H(4)MPT(+) to 5-formyl-
CC       H(4)MPT. {ECO:0000255|HAMAP-Rule:MF_00486}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O = H(+) +
CC         N(5)-formyl-5,6,7,8-tetrahydromethanopterin; Xref=Rhea:RHEA:19053,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:58018,
CC         ChEBI:CHEBI:58337; EC=3.5.4.27; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_00486};
CC   -!- PATHWAY: One-carbon metabolism; formaldehyde degradation; formate from
CC       formaldehyde (H(4)MPT route): step 3/5. {ECO:0000255|HAMAP-
CC       Rule:MF_00486}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00486}.
CC   -!- SIMILARITY: Belongs to the MCH family. {ECO:0000255|HAMAP-
CC       Rule:MF_00486}.
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DR   EMBL; CP001349; ACL60831.1; -; Genomic_DNA.
DR   RefSeq; WP_015932425.1; NC_011894.1.
DR   AlphaFoldDB; B8ITX3; -.
DR   SMR; B8ITX3; -.
DR   STRING; 460265.Mnod_6004; -.
DR   EnsemblBacteria; ACL60831; ACL60831; Mnod_6004.
DR   KEGG; mno:Mnod_6004; -.
DR   eggNOG; COG3252; Bacteria.
DR   HOGENOM; CLU_876031_0_0_5; -.
DR   OMA; DKGMFAP; -.
DR   OrthoDB; 1691428at2; -.
DR   UniPathway; UPA00562; UER00703.
DR   Proteomes; UP000008207; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0018759; F:methenyltetrahydromethanopterin cyclohydrolase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0046294; P:formaldehyde catabolic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0006730; P:one-carbon metabolic process; IEA:UniProtKB-UniRule.
DR   CDD; cd00545; MCH; 1.
DR   HAMAP; MF_00486; McH; 1.
DR   InterPro; IPR003209; METHMP_CycHdrlase.
DR   Pfam; PF02289; MCH; 1.
DR   TIGRFAMs; TIGR03120; one_C_mch; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Hydrolase; One-carbon metabolism; Reference proteome.
FT   CHAIN           1..324
FT                   /note="Methenyltetrahydromethanopterin cyclohydrolase"
FT                   /id="PRO_1000191112"
SQ   SEQUENCE   324 AA;  33355 MW;  9BFD07415EC68F05 CRC64;
     MSSTTVRPSV NALAAPLVEA LVADAAKLRL TVTANSGEAR LVNAGATVLG SIEAGRRIAE
     ICLGGLGTVT IAPTGPVAAW PYTITVHSTD PVLACLGSQY AGWSLADEGG TSGFFALGSG
     PGRAAAAVEH LFEELHYKDN AVQIALVLES ASAPPASVVA KVAEAAGVPL EAVTFIYAPT
     QSLAGSTQVV ARVLEVALHK AHSVGFDLAK IVDGIGAAPL SPPHPDFIQA MGRTNDAIIY
     GGRVQLFVDA EDADAKGLAE ALPSTTSRDH GAPFAEIFAR FNGDFYAIDS HLFSPAEVVV
     TSVRTGHSHR SGRLVPDLVE RSFA