ID   MCH_METOR               Reviewed;         324 AA.
AC   Q9RPW7;
DT   16-NOV-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   25-MAY-2022, entry version 77.
DE   RecName: Full=Methenyltetrahydromethanopterin cyclohydrolase;
DE            EC=3.5.4.27;
DE   AltName: Full=Methenyl-H4MPT cyclohydrolase;
GN   Name=mch;
OS   Methylobacterium organophilum.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Methylobacteriaceae; Methylobacterium.
OX   NCBI_TaxID=410;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 27886 / DSM 760 / JCM 2833 / NBRC 15689 / NCIMB 11278 / VKM
RC   B-2066 / XX;
RX   PubMed=10482517; DOI=10.1128/jb.181.18.5750-5757.1999;
RA   Vorholt J.A., Chistoserdova L.V., Stolyar S.M., Thauer R.K., Lidstrom M.E.;
RT   "Distribution of tetrahydromethanopterin-dependent enzymes in
RT   methylotrophic bacteria and phylogeny of methenyl tetrahydromethanopterin
RT   cyclohydrolases.";
RL   J. Bacteriol. 181:5750-5757(1999).
CC   -!- FUNCTION: Catalyzes the hydrolysis of methenyl-H(4)MPT(+) to 5-formyl-
CC       H(4)MPT. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O = H(+) +
CC         N(5)-formyl-5,6,7,8-tetrahydromethanopterin; Xref=Rhea:RHEA:19053,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:58018,
CC         ChEBI:CHEBI:58337; EC=3.5.4.27;
CC   -!- PATHWAY: One-carbon metabolism; formaldehyde degradation; formate from
CC       formaldehyde (H(4)MPT route): step 3/5.
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the MCH family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AF142650; AAD55900.1; -; Genomic_DNA.
DR   RefSeq; WP_012317537.1; NZ_QEKZ01000004.1.
DR   AlphaFoldDB; Q9RPW7; -.
DR   SMR; Q9RPW7; -.
DR   UniPathway; UPA00562; UER00703.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0018759; F:methenyltetrahydromethanopterin cyclohydrolase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0046294; P:formaldehyde catabolic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0006730; P:one-carbon metabolic process; IEA:UniProtKB-UniRule.
DR   CDD; cd00545; MCH; 1.
DR   HAMAP; MF_00486; McH; 1.
DR   InterPro; IPR003209; METHMP_CycHdrlase.
DR   Pfam; PF02289; MCH; 1.
DR   TIGRFAMs; TIGR03120; one_C_mch; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Hydrolase; One-carbon metabolism.
FT   CHAIN           1..324
FT                   /note="Methenyltetrahydromethanopterin cyclohydrolase"
FT                   /id="PRO_0000140892"
SQ   SEQUENCE   324 AA;  33268 MW;  A11F6103F2E84CDB CRC64;
     MSASQSYPSI NALSGPLVER LVADAPTLRL SVSQAAGGAR MVDAGAQARG SIEAGRRIAE
     ICLGGLGTVT IAPSGPIASW PYSVTVHSAD PVLACLGSQY AGWSLADETG DSGFFALGSG
     PGRAVAAVED LYQELGFRDS ATKTALVLEA AGGPPESVVA KVAEASGLKP EDLTFIYAPT
     QSLAGSTQVV ARVLEVALHK AHTVGFDLHA IVDGIGSAPL SPPHPDFIKA MGRTNDAIIY
     GGRVQLFVDA DDADAKQLAE QLPSTTSSDH GAPFAEIFAR VNGDFYKIDG ALFSPAEAIV
     TSVRSGATYR GGRLEPTLVD ASFA