ID   MCH_METTO               Reviewed;         136 AA.
AC   Q9RPW3;
DT   16-NOV-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   25-MAY-2022, entry version 66.
DE   RecName: Full=Methenyltetrahydromethanopterin cyclohydrolase;
DE            EC=3.5.4.27;
DE   AltName: Full=Methenyl-H4MPT cyclohydrolase;
DE   Flags: Fragment;
GN   Name=mch;
OS   Methylococcus thermophilus.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Methylococcales;
OC   Methylococcaceae; Methylococcus.
OX   NCBI_TaxID=53405;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=IIIp;
RX   PubMed=10482517; DOI=10.1128/jb.181.18.5750-5757.1999;
RA   Vorholt J.A., Chistoserdova L.V., Stolyar S.M., Thauer R.K., Lidstrom M.E.;
RT   "Distribution of tetrahydromethanopterin-dependent enzymes in
RT   methylotrophic bacteria and phylogeny of methenyl tetrahydromethanopterin
RT   cyclohydrolases.";
RL   J. Bacteriol. 181:5750-5757(1999).
CC   -!- FUNCTION: Catalyzes the hydrolysis of methenyl-H(4)MPT(+) to 5-formyl-
CC       H(4)MPT. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O = H(+) +
CC         N(5)-formyl-5,6,7,8-tetrahydromethanopterin; Xref=Rhea:RHEA:19053,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:58018,
CC         ChEBI:CHEBI:58337; EC=3.5.4.27;
CC   -!- PATHWAY: One-carbon metabolism; formaldehyde degradation; formate from
CC       formaldehyde (H(4)MPT route): step 3/5.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the MCH family. {ECO:0000305}.
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DR   EMBL; AF142654; AAD55904.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q9RPW3; -.
DR   SMR; Q9RPW3; -.
DR   UniPathway; UPA00562; UER00703.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0018759; F:methenyltetrahydromethanopterin cyclohydrolase activity; IEA:UniProtKB-EC.
DR   GO; GO:0046294; P:formaldehyde catabolic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0006730; P:one-carbon metabolic process; IEA:UniProtKB-KW.
DR   InterPro; IPR003209; METHMP_CycHdrlase.
DR   Pfam; PF02289; MCH; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Hydrolase; One-carbon metabolism.
FT   CHAIN           <1..>136
FT                   /note="Methenyltetrahydromethanopterin cyclohydrolase"
FT                   /id="PRO_0000140895"
FT   NON_TER         1
FT   NON_TER         136
SQ   SEQUENCE   136 AA;  14337 MW;  33BB5F41C53DFB69 CRC64;
     SLSHGEGKNA FYALGSGPAR ALATKVKDGN EEPVEELYKE LGYRDHSNET AIVMEVDKVP
     PVEVIEKIAK ACKVDASGVH VILTPTSSLA GGMQVVGRVL EVALHKAHSL HFPLGNIIDG
     TGTAPVPPPH PNFVKA