ID   MCH_METTR               Reviewed;         260 AA.
AC   Q9RPD4;
DT   16-NOV-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   25-MAY-2022, entry version 76.
DE   RecName: Full=Methenyltetrahydromethanopterin cyclohydrolase;
DE            EC=3.5.4.27;
DE   AltName: Full=Methenyl-H4MPT cyclohydrolase;
DE   Flags: Fragment;
GN   Name=mch;
OS   Methylosinus trichosporium.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Methylocystaceae; Methylosinus.
OX   NCBI_TaxID=426;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 35070 / NCIMB 11131 / ACM 3311 / OB3b;
RX   PubMed=10482517; DOI=10.1128/jb.181.18.5750-5757.1999;
RA   Vorholt J.A., Chistoserdova L.V., Stolyar S.M., Thauer R.K., Lidstrom M.E.;
RT   "Distribution of tetrahydromethanopterin-dependent enzymes in
RT   methylotrophic bacteria and phylogeny of methenyl tetrahydromethanopterin
RT   cyclohydrolases.";
RL   J. Bacteriol. 181:5750-5757(1999).
CC   -!- FUNCTION: Catalyzes the hydrolysis of methenyl-H(4)MPT(+) to 5-formyl-
CC       H(4)MPT. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O = H(+) +
CC         N(5)-formyl-5,6,7,8-tetrahydromethanopterin; Xref=Rhea:RHEA:19053,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:58018,
CC         ChEBI:CHEBI:58337; EC=3.5.4.27;
CC   -!- PATHWAY: One-carbon metabolism; formaldehyde degradation; formate from
CC       formaldehyde (H(4)MPT route): step 3/5.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the MCH family. {ECO:0000305}.
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DR   EMBL; AF162786; AAD56174.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q9RPD4; -.
DR   SMR; Q9RPD4; -.
DR   UniPathway; UPA00562; UER00703.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0018759; F:methenyltetrahydromethanopterin cyclohydrolase activity; IEA:UniProtKB-EC.
DR   GO; GO:0046294; P:formaldehyde catabolic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0006730; P:one-carbon metabolic process; IEA:UniProtKB-KW.
DR   HAMAP; MF_00486; McH; 1.
DR   InterPro; IPR003209; METHMP_CycHdrlase.
DR   Pfam; PF02289; MCH; 1.
DR   TIGRFAMs; TIGR03120; one_C_mch; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Hydrolase; One-carbon metabolism.
FT   CHAIN           <1..260
FT                   /note="Methenyltetrahydromethanopterin cyclohydrolase"
FT                   /id="PRO_0000140896"
FT   NON_TER         1
SQ   SEQUENCE   260 AA;  27622 MW;  BD647C0DBF03C6A8 CRC64;
     ICLGGLGKVT LAPAPGQTNW PFWLTVTSND PVVACLASQY AGWSLSHEKF FALGSGPGRS
     LARKEPLFQE LPYEDSASRA TIVLEAGAPP PEPVVAKVAE SCGVSPDKLA FIYAPTQSLA
     GSVQVVGRVL EVALHKAHEL KFPLEHIVDG IATAPLSPPH PDFVTAMGRT NDAIIYSGRA
     HLFVRGSAEA AKALAEKLPS SNSRDYGRPF AEIFKAYKGE FYKIDPSLFS PAEAIVTAVE
     TGETFRAGAI DEKLLDASFG