MCH_METTR
ID MCH_METTR Reviewed; 260 AA.
AC Q9RPD4;
DT 16-NOV-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 25-MAY-2022, entry version 76.
DE RecName: Full=Methenyltetrahydromethanopterin cyclohydrolase;
DE EC=3.5.4.27;
DE AltName: Full=Methenyl-H4MPT cyclohydrolase;
DE Flags: Fragment;
GN Name=mch;
OS Methylosinus trichosporium.
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Methylocystaceae; Methylosinus.
OX NCBI_TaxID=426;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 35070 / NCIMB 11131 / ACM 3311 / OB3b;
RX PubMed=10482517; DOI=10.1128/jb.181.18.5750-5757.1999;
RA Vorholt J.A., Chistoserdova L.V., Stolyar S.M., Thauer R.K., Lidstrom M.E.;
RT "Distribution of tetrahydromethanopterin-dependent enzymes in
RT methylotrophic bacteria and phylogeny of methenyl tetrahydromethanopterin
RT cyclohydrolases.";
RL J. Bacteriol. 181:5750-5757(1999).
CC -!- FUNCTION: Catalyzes the hydrolysis of methenyl-H(4)MPT(+) to 5-formyl-
CC H(4)MPT. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O = H(+) +
CC N(5)-formyl-5,6,7,8-tetrahydromethanopterin; Xref=Rhea:RHEA:19053,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:58018,
CC ChEBI:CHEBI:58337; EC=3.5.4.27;
CC -!- PATHWAY: One-carbon metabolism; formaldehyde degradation; formate from
CC formaldehyde (H(4)MPT route): step 3/5.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the MCH family. {ECO:0000305}.
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DR EMBL; AF162786; AAD56174.1; -; Genomic_DNA.
DR AlphaFoldDB; Q9RPD4; -.
DR SMR; Q9RPD4; -.
DR UniPathway; UPA00562; UER00703.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0018759; F:methenyltetrahydromethanopterin cyclohydrolase activity; IEA:UniProtKB-EC.
DR GO; GO:0046294; P:formaldehyde catabolic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0006730; P:one-carbon metabolic process; IEA:UniProtKB-KW.
DR HAMAP; MF_00486; McH; 1.
DR InterPro; IPR003209; METHMP_CycHdrlase.
DR Pfam; PF02289; MCH; 1.
DR TIGRFAMs; TIGR03120; one_C_mch; 1.
PE 3: Inferred from homology;
KW Cytoplasm; Hydrolase; One-carbon metabolism.
FT CHAIN <1..260
FT /note="Methenyltetrahydromethanopterin cyclohydrolase"
FT /id="PRO_0000140896"
FT NON_TER 1
SQ SEQUENCE 260 AA; 27622 MW; BD647C0DBF03C6A8 CRC64;
ICLGGLGKVT LAPAPGQTNW PFWLTVTSND PVVACLASQY AGWSLSHEKF FALGSGPGRS
LARKEPLFQE LPYEDSASRA TIVLEAGAPP PEPVVAKVAE SCGVSPDKLA FIYAPTQSLA
GSVQVVGRVL EVALHKAHEL KFPLEHIVDG IATAPLSPPH PDFVTAMGRT NDAIIYSGRA
HLFVRGSAEA AKALAEKLPS SNSRDYGRPF AEIFKAYKGE FYKIDPSLFS PAEAIVTAVE
TGETFRAGAI DEKLLDASFG