ID   MCH_XANAU               Reviewed;         337 AA.
AC   Q9RQ01;
DT   16-NOV-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   25-MAY-2022, entry version 79.
DE   RecName: Full=Methenyltetrahydromethanopterin cyclohydrolase;
DE            EC=3.5.4.27;
DE   AltName: Full=Methenyl-H4MPT cyclohydrolase;
GN   Name=mch;
OS   Xanthobacter autotrophicus.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Xanthobacteraceae; Xanthobacter.
OX   NCBI_TaxID=280;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 35674 / DSM 432 / JCM 1202 / LMG 7043;
RX   PubMed=10482517; DOI=10.1128/jb.181.18.5750-5757.1999;
RA   Vorholt J.A., Chistoserdova L.V., Stolyar S.M., Thauer R.K., Lidstrom M.E.;
RT   "Distribution of tetrahydromethanopterin-dependent enzymes in
RT   methylotrophic bacteria and phylogeny of methenyl tetrahydromethanopterin
RT   cyclohydrolases.";
RL   J. Bacteriol. 181:5750-5757(1999).
CC   -!- FUNCTION: Catalyzes the hydrolysis of methenyl-H(4)MPT(+) to 5-formyl-
CC       H(4)MPT. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O = H(+) +
CC         N(5)-formyl-5,6,7,8-tetrahydromethanopterin; Xref=Rhea:RHEA:19053,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:58018,
CC         ChEBI:CHEBI:58337; EC=3.5.4.27;
CC   -!- PATHWAY: One-carbon metabolism; formaldehyde degradation; formate from
CC       formaldehyde (H(4)MPT route): step 3/5.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the MCH family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AF139593; AAD55896.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q9RQ01; -.
DR   SMR; Q9RQ01; -.
DR   UniPathway; UPA00562; UER00703.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0018759; F:methenyltetrahydromethanopterin cyclohydrolase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0046294; P:formaldehyde catabolic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0006730; P:one-carbon metabolic process; IEA:UniProtKB-UniRule.
DR   CDD; cd00545; MCH; 1.
DR   HAMAP; MF_00486; McH; 1.
DR   InterPro; IPR003209; METHMP_CycHdrlase.
DR   Pfam; PF02289; MCH; 1.
DR   TIGRFAMs; TIGR03120; one_C_mch; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Hydrolase; One-carbon metabolism.
FT   CHAIN           1..337
FT                   /note="Methenyltetrahydromethanopterin cyclohydrolase"
FT                   /id="PRO_0000140898"
SQ   SEQUENCE   337 AA;  34783 MW;  AC53B27E8DDFBF08 CRC64;
     MTSQATSQAT SPALTTPVDA APQISLATCV APLVEALIRD ADALRLKVSR GPRDALIVDA
     GITAAGGLEA GRRIAEICLG GLGRVALVPT GRFSPWDTLA SVSTSGPVLA CLGSQYAGWS
     LAAGDFFALG SGPGRAIAAV ETLYGELGYR DRGEKVVLVL ETAVVPPAEV VDEIAARCAV
     APSDITLILT PTSSLAGTVQ IVARVLEVAL HKAHALHFPL EHIADGVGSA PICPPSPDFL
     TAMGRTNDAV LYGGDVHLFV HGPAEAAKDL ATRLPSLASR DYGRPFGEIF AGYDCDFYKV
     DPLLFSPARV TVTAIDHGES FTAGGFDPIL IARSFGG