MCJD_ECOLX
ID MCJD_ECOLX Reviewed; 580 AA.
AC Q9X2W0;
DT 29-MAR-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1999, sequence version 1.
DT 03-AUG-2022, entry version 94.
DE RecName: Full=Microcin-J25 export ATP-binding/permease protein McjD;
DE AltName: Full=Microcin-J25 immunity protein;
DE AltName: Full=Microcin-J25 secretion ATP-binding protein McjD;
GN Name=mcjD;
OS Escherichia coli.
OG Plasmid pTUC100.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=562;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC STRAIN=AY25;
RX PubMed=10198038; DOI=10.1128/jb.181.8.2659-2662.1999;
RA Solbiati J.O., Ciaccio M., Farias R.N., Gonzalez-Pastor J.E., Moreno F.,
RA Salomon R.A.;
RT "Sequence analysis of the four plasmid genes required to produce the
RT circular peptide antibiotic microcin J25.";
RL J. Bacteriol. 181:2659-2662(1999).
RN [2]
RP FUNCTION.
RC STRAIN=AY25;
RX PubMed=8655570; DOI=10.1128/jb.178.12.3661-3663.1996;
RA Solbiati J.O., Ciaccio M., Farias R.N., Salomon R.A.;
RT "Genetic analysis of plasmid determinants for microcin J25 production and
RT immunity.";
RL J. Bacteriol. 178:3661-3663(1996).
CC -!- FUNCTION: Is able to protect a cell, which harbors the plasmid pTUC100
CC encoding microcin J25, against microcin J25. Is required for microcin
CC J25 export out of the producing cells. {ECO:0000269|PubMed:10198038,
CC ECO:0000269|PubMed:8655570}.
CC -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Multi-pass
CC membrane protein {ECO:0000255|PROSITE-ProRule:PRU00441}.
CC -!- SIMILARITY: Belongs to the ABC transporter superfamily. {ECO:0000305}.
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DR EMBL; AF061787; AAD28497.1; -; Genomic_DNA.
DR PDB; 4PL0; X-ray; 2.70 A; A/B=1-580.
DR PDB; 5EG1; X-ray; 3.42 A; A/B=1-580.
DR PDB; 5OFP; X-ray; 4.71 A; A=1-580.
DR PDB; 5OFR; X-ray; 3.40 A; A/B=1-580.
DR PDBsum; 4PL0; -.
DR PDBsum; 5EG1; -.
DR PDBsum; 5OFP; -.
DR PDBsum; 5OFR; -.
DR AlphaFoldDB; Q9X2W0; -.
DR SMR; Q9X2W0; -.
DR TCDB; 3.A.1.118.1; the atp-binding cassette (abc) superfamily.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0140359; F:ABC-type transporter activity; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0030153; P:bacteriocin immunity; IEA:UniProtKB-KW.
DR GO; GO:0043213; P:bacteriocin transport; IEA:UniProtKB-KW.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR Gene3D; 1.20.1560.10; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR011527; ABC1_TM_dom.
DR InterPro; IPR036640; ABC1_TM_sf.
DR InterPro; IPR003439; ABC_transporter-like_ATP-bd.
DR InterPro; IPR017871; ABC_transporter-like_CS.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF00664; ABC_membrane; 1.
DR Pfam; PF00005; ABC_tran; 1.
DR SMART; SM00382; AAA; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR SUPFAM; SSF90123; SSF90123; 1.
DR PROSITE; PS50929; ABC_TM1F; 1.
DR PROSITE; PS00211; ABC_TRANSPORTER_1; 1.
DR PROSITE; PS50893; ABC_TRANSPORTER_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; ATP-binding; Bacteriocin immunity; Bacteriocin transport;
KW Cell inner membrane; Cell membrane; Membrane; Nucleotide-binding; Plasmid;
KW Protein transport; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..580
FT /note="Microcin-J25 export ATP-binding/permease protein
FT McjD"
FT /id="PRO_0000092491"
FT TRANSMEM 25..45
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TRANSMEM 66..86
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TRANSMEM 143..163
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TRANSMEM 167..187
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TRANSMEM 261..281
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TRANSMEM 286..306
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT DOMAIN 25..312
FT /note="ABC transmembrane type-1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT DOMAIN 345..578
FT /note="ABC transporter"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00434"
FT BINDING 378..385
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00434"
FT HELIX 5..8
FT /evidence="ECO:0007829|PDB:4PL0"
FT HELIX 10..15
FT /evidence="ECO:0007829|PDB:4PL0"
FT HELIX 19..53
FT /evidence="ECO:0007829|PDB:4PL0"
FT TURN 54..56
FT /evidence="ECO:0007829|PDB:5OFR"
FT HELIX 59..110
FT /evidence="ECO:0007829|PDB:4PL0"
FT HELIX 114..117
FT /evidence="ECO:0007829|PDB:4PL0"
FT HELIX 122..145
FT /evidence="ECO:0007829|PDB:4PL0"
FT HELIX 148..163
FT /evidence="ECO:0007829|PDB:4PL0"
FT HELIX 167..214
FT /evidence="ECO:0007829|PDB:4PL0"
FT HELIX 216..221
FT /evidence="ECO:0007829|PDB:4PL0"
FT HELIX 225..277
FT /evidence="ECO:0007829|PDB:4PL0"
FT HELIX 283..322
FT /evidence="ECO:0007829|PDB:4PL0"
FT TURN 323..325
FT /evidence="ECO:0007829|PDB:4PL0"
FT STRAND 345..368
FT /evidence="ECO:0007829|PDB:4PL0"
FT STRAND 373..377
FT /evidence="ECO:0007829|PDB:4PL0"
FT HELIX 384..391
FT /evidence="ECO:0007829|PDB:4PL0"
FT STRAND 399..404
FT /evidence="ECO:0007829|PDB:4PL0"
FT HELIX 409..411
FT /evidence="ECO:0007829|PDB:4PL0"
FT HELIX 414..420
FT /evidence="ECO:0007829|PDB:4PL0"
FT STRAND 421..424
FT /evidence="ECO:0007829|PDB:4PL0"
FT STRAND 432..434
FT /evidence="ECO:0007829|PDB:4PL0"
FT HELIX 435..440
FT /evidence="ECO:0007829|PDB:4PL0"
FT HELIX 448..457
FT /evidence="ECO:0007829|PDB:4PL0"
FT STRAND 461..463
FT /evidence="ECO:0007829|PDB:4PL0"
FT HELIX 476..478
FT /evidence="ECO:0007829|PDB:4PL0"
FT HELIX 483..496
FT /evidence="ECO:0007829|PDB:4PL0"
FT STRAND 500..506
FT /evidence="ECO:0007829|PDB:4PL0"
FT TURN 507..510
FT /evidence="ECO:0007829|PDB:4PL0"
FT HELIX 513..526
FT /evidence="ECO:0007829|PDB:4PL0"
FT STRAND 527..529
FT /evidence="ECO:0007829|PDB:5OFR"
FT STRAND 531..535
FT /evidence="ECO:0007829|PDB:4PL0"
FT STRAND 537..539
FT /evidence="ECO:0007829|PDB:4PL0"
FT TURN 540..544
FT /evidence="ECO:0007829|PDB:4PL0"
FT STRAND 545..552
FT /evidence="ECO:0007829|PDB:4PL0"
FT STRAND 555..558
FT /evidence="ECO:0007829|PDB:4PL0"
FT HELIX 565..568
FT /evidence="ECO:0007829|PDB:4PL0"
FT HELIX 570..576
FT /evidence="ECO:0007829|PDB:4PL0"
SQ SEQUENCE 580 AA; 65425 MW; B0F6D0E929F203C6 CRC64;
MERKQKNSLF NYIYSLMDVR GKFLFFSMLF ITSLSSIIIS ISPLILAKIT DLLSGSLSNF
SYEYLVLLAC LYMFCVISNK ASVFLFMILQ SSLRINMQKK MSLKYLRELY NENITNLSKN
NAGYTTQSLN QASNDIYILV RNVSQNILSP VIQLISTIVV VLSTKDWFSA GVFFLYILVF
VIFNTRLTGS LASLRKHSMD ITLNSYSLLS DTVDNMIAAK KNNALRLISE RYEDALTQEN
NAQKKYWLLS SKVLLLNSLL AVILFGSVFI YNILGVLNGV VSIGHFIMIT SYIILLSTPV
ENIGALLSEI RQSMSSLAGF IQRHAENKAT SPSIPFLNME RKLNLSIREL SFSYSDDKKI
LNSVSLDLFT GKMYSLTGPS GSGKSTLVKI ISGYYKNYFG DIYLNDISLR NISDEDLNDA
IYYLTQDDYI FMDTLRFNLR LANYDASENE IFKVLKLANL SVVNNEPVSL DTHLINRGNN
YSGGQKQRIS LARLFLRKPA IIIIDEATSA LDYINESEIL SSIRTHFPDA LIINISHRIN
LLECSDCVYV LNEGNIVASG HFRDLMVSNE YISGLASVTE
