MCM10_DROME
ID MCM10_DROME Reviewed; 776 AA.
AC Q9VIE6; Q95RC7;
DT 20-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 2.
DT 03-AUG-2022, entry version 140.
DE RecName: Full=Protein MCM10 homolog;
DE AltName: Full=Sensitized chromosome inheritance modifier 19;
GN Name=Mcm10; Synonyms=Scim19; ORFNames=CG9241;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 555-776.
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4]
RP FUNCTION, INTERACTION WITH MCM2; DUP; ORC2; CDC45 AND SU(VAR)205, AND
RP SELF-ASSOCIATION.
RX PubMed=12808023; DOI=10.1091/mbc.e02-11-0706;
RA Christensen T.W., Tye B.K.;
RT "Drosophila MCM10 interacts with members of the prereplication complex and
RT is required for proper chromosome condensation.";
RL Mol. Biol. Cell 14:2206-2215(2003).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-87 AND SER-88, AND
RP IDENTIFICATION BY MASS SPECTROMETRY.
RC TISSUE=Embryo;
RX PubMed=18327897; DOI=10.1021/pr700696a;
RA Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
RT "Phosphoproteome analysis of Drosophila melanogaster embryos.";
RL J. Proteome Res. 7:1675-1682(2008).
CC -!- FUNCTION: Proposed to be involved in DNA replication and to participate
CC in the activation of the pre-replication complex (pre-RC). May be
CC involved in chromosome condensation. {ECO:0000269|PubMed:12808023}.
CC -!- SUBUNIT: Self-associates. Interacts with Mcm2, dup, Orc2, CDC45L and
CC Su(var)205. {ECO:0000269|PubMed:12808023}.
CC -!- INTERACTION:
CC Q9VIE6; O96989: Cdc45; NbExp=2; IntAct=EBI-91264, EBI-156880;
CC Q9VIE6; Q7JVY2: dup; NbExp=2; IntAct=EBI-91264, EBI-164632;
CC Q9VIE6; P49735: Mcm2; NbExp=2; IntAct=EBI-91264, EBI-138228;
CC Q9VIE6; Q24168: Orc2; NbExp=2; IntAct=EBI-91264, EBI-179453;
CC Q9VIE6; P05205: Su(var)205; NbExp=2; IntAct=EBI-91264, EBI-155532;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- DOMAIN: The zinc finger-like domain binds a zinc ion and is involved in
CC self-association. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the MCM10 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAL29025.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AE014134; AAF53976.2; -; Genomic_DNA.
DR EMBL; AY061477; AAL29025.1; ALT_INIT; mRNA.
DR RefSeq; NP_610097.2; NM_136253.4.
DR AlphaFoldDB; Q9VIE6; -.
DR SMR; Q9VIE6; -.
DR BioGRID; 61343; 8.
DR DIP; DIP-20713N; -.
DR IntAct; Q9VIE6; 12.
DR STRING; 7227.FBpp0081004; -.
DR iPTMnet; Q9VIE6; -.
DR PaxDb; Q9VIE6; -.
DR DNASU; 35390; -.
DR EnsemblMetazoa; FBtr0081475; FBpp0081004; FBgn0032929.
DR GeneID; 35390; -.
DR KEGG; dme:Dmel_CG9241; -.
DR CTD; 55388; -.
DR FlyBase; FBgn0032929; Mcm10.
DR VEuPathDB; VectorBase:FBgn0032929; -.
DR eggNOG; KOG3056; Eukaryota.
DR GeneTree; ENSGT00390000007134; -.
DR HOGENOM; CLU_014680_1_1_1; -.
DR InParanoid; Q9VIE6; -.
DR OMA; VFGLRMI; -.
DR OrthoDB; 758449at2759; -.
DR PhylomeDB; Q9VIE6; -.
DR Reactome; R-DME-176187; Activation of ATR in response to replication stress.
DR Reactome; R-DME-68962; Activation of the pre-replicative complex.
DR SignaLink; Q9VIE6; -.
DR BioGRID-ORCS; 35390; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 35390; -.
DR PRO; PR:Q9VIE6; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0032929; Expressed in egg cell and 16 other tissues.
DR ExpressionAtlas; Q9VIE6; baseline and differential.
DR Genevisible; Q9VIE6; DM.
DR GO; GO:0005634; C:nucleus; IDA:FlyBase.
DR GO; GO:0031298; C:replication fork protection complex; IBA:GO_Central.
DR GO; GO:0003688; F:DNA replication origin binding; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0003697; F:single-stranded DNA binding; IBA:GO_Central.
DR GO; GO:0030261; P:chromosome condensation; IMP:FlyBase.
DR GO; GO:0042023; P:DNA endoreduplication; IMP:FlyBase.
DR GO; GO:0006260; P:DNA replication; IMP:FlyBase.
DR GO; GO:0006270; P:DNA replication initiation; IBA:GO_Central.
DR GO; GO:0035214; P:eye-antennal disc development; IMP:FlyBase.
DR GO; GO:1901693; P:negative regulation of compound eye retinal cell apoptotic process; IMP:FlyBase.
DR GO; GO:0045931; P:positive regulation of mitotic cell cycle; IMP:FlyBase.
DR GO; GO:0045678; P:positive regulation of R7 cell differentiation; IMP:FlyBase.
DR Gene3D; 2.40.50.140; -; 1.
DR InterPro; IPR040184; Mcm10.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR015411; Rep_factor_Mcm10_C.
DR InterPro; IPR015408; Znf_Mcm10/DnaG.
DR PANTHER; PTHR13454; PTHR13454; 1.
DR Pfam; PF09332; Mcm10; 1.
DR Pfam; PF09329; zf-primase; 1.
DR SMART; SM01280; Mcm10; 1.
PE 1: Evidence at protein level;
KW DNA replication; Metal-binding; Nucleus; Phosphoprotein;
KW Reference proteome; Zinc; Zinc-finger.
FT CHAIN 1..776
FT /note="Protein MCM10 homolog"
FT /id="PRO_0000278325"
FT REGION 30..49
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 331..356
FT /note="Zinc finger-like"
FT REGION 430..468
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 487..521
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 487..503
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 87
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 88
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
SQ SEQUENCE 776 AA; 86524 MW; 2076F7C06FCDAD9C CRC64;
MGPAQKSGTD ISIDDEEEIL ALEKLLGAAE NENTKSAESE KAKPTAPILV PKLREDNSFA
NAFTFEKIVK PEKQKNAAII KEPELDSSDD EEVKNFLERK YNEYGSDINK RLKQQQENAY
ESKVAREVDQ ELKKSIHVVT STPQPLKNPH NPIKRQSAVS TTFQRPPPVA AAVASTSQSS
APVSAVFTDP VFGLRMINPL VSSSLLQERM TGRKPVPFSG VAYHIERGDL AKDWVIAGAL
VSKNPVKNTK KGDPYSTWKL SDLRGEVKTI SLFLFKEAHK SLWKTAEGLC LAVLNPTIFE
RRAGSSDVAC LSIDSSQKVM ILGQSKDLGT CRATKKNGDK CTSVVNLTDC DYCIFHVKQE
YGKMSRRSEL QSATAGRGIN ELRNKVLGKN EVFYGGQTFT AVPARKSAKL ITKERDRLSM
LAGYDVSPFA HTANHTSKPK TAEPTKIPYA ERGGPVSRLA GGVEASRKQR VQDLERLRLL
KEENERFEKK KQAEGHVLGS DNKKESEAGT PAVSMPTTPV PDKFKNRGFS FDASLTPKLS
GSENFSFEIN VGSRQAQNAK LKAAALLKKK PLEKINPNST RGSESGKRRA IDELNEKFSS
SAKRQKIDED DRELMRKSRI EKIMAATSSH TNLVEMRERE AQEEYFNKLE RKEAMEEKML
TTYKMPCKAV ICQVCKYTAF SASDRCKEQK HPLKVVDAEK RFFQCKDCGN RTTTVFKLPK
QSCKNCKGSR WQRTAMIREK KILTGRETLS VRGDEETFMG CLAGSANLNL LVPDEE
