MCM2_ORYSJ
ID MCM2_ORYSJ Reviewed; 961 AA.
AC Q2R482; A0A0N7KSX8;
DT 16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT 24-JAN-2006, sequence version 1.
DT 03-AUG-2022, entry version 140.
DE RecName: Full=DNA replication licensing factor MCM2 {ECO:0000305};
DE EC=3.6.4.12 {ECO:0000305};
DE AltName: Full=Minichromosome maintenance protein 2 {ECO:0000303|PubMed:18755073};
DE Short=OsMCM2 {ECO:0000303|PubMed:18755073};
GN Name=MCM2 {ECO:0000303|PubMed:18755073};
GN OrderedLocusNames=Os11g0484300, LOC_Os11g29380;
GN ORFNames=OsJ_33907 {ECO:0000312|EMBL:EEE52107.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16188032; DOI=10.1186/1741-7007-3-20;
RG The rice chromosomes 11 and 12 sequencing consortia;
RT "The sequence of rice chromosomes 11 and 12, rich in disease resistance
RT genes and recent gene duplications.";
RL BMC Biol. 3:20-20(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [7]
RP GENE FAMILY.
RX PubMed=17556508; DOI=10.1104/pp.107.101105;
RA Shultz R.W., Tatineni V.M., Hanley-Bowdoin L., Thompson W.F.;
RT "Genome-wide analysis of the core DNA replication machinery in the higher
RT plants Arabidopsis and rice.";
RL Plant Physiol. 144:1697-1714(2007).
RN [8]
RP FUNCTION, INTERACTION WITH CSN5, AND TISSUE SPECIFICITY.
RX PubMed=18755073; DOI=10.5483/bmbrep.2008.41.8.581;
RA Cho J.H., Kim H.B., Kim H.S., Choi S.B.;
RT "Identification and characterization of a rice MCM2 homologue required for
RT DNA replication.";
RL BMB Rep. 41:581-586(2008).
CC -!- FUNCTION: Probable component of the MCM2-7 complex (MCM complex) that
CC may function as a DNA helicase and which is essential to undergo a
CC single round of replication initiation and elongation per cell cycle in
CC eukaryotic cells. Can complement the fission yeast mcm2 mutant.
CC {ECO:0000269|PubMed:18755073}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC Evidence={ECO:0000305};
CC -!- SUBUNIT: Component of the minichromosome maintenance (MCM) complex, a
CC heterotetramer composed of MCM2, MCM3, MCM4, MCM5, MCM6 and MCM7 (By
CC similarity). Interacts with CSN5 (PubMed:18755073).
CC {ECO:0000250|UniProtKB:Q9LPD9, ECO:0000269|PubMed:18755073}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Widely expressed, with higher expression in
CC developing tissues. {ECO:0000269|PubMed:18755073}.
CC -!- SIMILARITY: Belongs to the MCM family. {ECO:0000305}.
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DR EMBL; DP000010; ABA93692.1; -; Genomic_DNA.
DR EMBL; AP008217; BAF28273.1; -; Genomic_DNA.
DR EMBL; AP014967; BAT14058.1; -; Genomic_DNA.
DR EMBL; CM000148; EEE52107.1; -; Genomic_DNA.
DR EMBL; AK121422; BAH00480.1; -; mRNA.
DR RefSeq; XP_015615188.1; XM_015759702.1.
DR AlphaFoldDB; Q2R482; -.
DR SMR; Q2R482; -.
DR STRING; 4530.OS11T0484300-01; -.
DR iPTMnet; Q2R482; -.
DR PaxDb; Q2R482; -.
DR PRIDE; Q2R482; -.
DR EnsemblPlants; Os11t0484300-01; Os11t0484300-01; Os11g0484300.
DR GeneID; 4350526; -.
DR Gramene; Os11t0484300-01; Os11t0484300-01; Os11g0484300.
DR KEGG; osa:4350526; -.
DR eggNOG; KOG0477; Eukaryota.
DR HOGENOM; CLU_000995_0_1_1; -.
DR InParanoid; Q2R482; -.
DR OMA; DCVKCGY; -.
DR OrthoDB; 266497at2759; -.
DR PlantReactome; R-OSA-9640882; Assembly of pre-replication complex.
DR PlantReactome; R-OSA-9645850; Activation of pre-replication complex.
DR PlantReactome; R-OSA-9675824; DNA replication Initiation.
DR Proteomes; UP000000763; Chromosome 11.
DR Proteomes; UP000007752; Chromosome 11.
DR Proteomes; UP000059680; Chromosome 11.
DR Genevisible; Q2R482; OS.
DR GO; GO:0000785; C:chromatin; IEA:EnsemblPlants.
DR GO; GO:0042555; C:MCM complex; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0000347; C:THO complex; IEA:EnsemblPlants.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003678; F:DNA helicase activity; IEA:UniProtKB-EC.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0003697; F:single-stranded DNA binding; IBA:GO_Central.
DR GO; GO:0006268; P:DNA unwinding involved in DNA replication; IDA:UniProtKB.
DR GO; GO:0000727; P:double-strand break repair via break-induced replication; IBA:GO_Central.
DR GO; GO:0009793; P:embryo development ending in seed dormancy; IEA:EnsemblPlants.
DR GO; GO:1902975; P:mitotic DNA replication initiation; IBA:GO_Central.
DR GO; GO:1905775; P:negative regulation of DNA helicase activity; IEA:InterPro.
DR GO; GO:0042127; P:regulation of cell population proliferation; IEA:EnsemblPlants.
DR GO; GO:0010082; P:regulation of root meristem growth; IEA:EnsemblPlants.
DR Gene3D; 2.40.50.140; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR031327; MCM.
DR InterPro; IPR008045; MCM2.
DR InterPro; IPR018525; MCM_CS.
DR InterPro; IPR001208; MCM_dom.
DR InterPro; IPR041562; MCM_lid.
DR InterPro; IPR027925; MCM_N.
DR InterPro; IPR033762; MCM_OB.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR11630; PTHR11630; 1.
DR Pfam; PF00493; MCM; 1.
DR Pfam; PF12619; MCM2_N; 1.
DR Pfam; PF17855; MCM_lid; 1.
DR Pfam; PF14551; MCM_N; 1.
DR Pfam; PF17207; MCM_OB; 1.
DR PRINTS; PR01657; MCMFAMILY.
DR PRINTS; PR01658; MCMPROTEIN2.
DR SMART; SM00350; MCM; 1.
DR SUPFAM; SSF50249; SSF50249; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS00847; MCM_1; 1.
DR PROSITE; PS50051; MCM_2; 1.
PE 1: Evidence at protein level;
KW ATP-binding; Cell cycle; DNA replication; DNA-binding; Helicase; Hydrolase;
KW Metal-binding; Nucleotide-binding; Nucleus; Reference proteome; Zinc;
KW Zinc-finger.
FT CHAIN 1..961
FT /note="DNA replication licensing factor MCM2"
FT /id="PRO_0000425987"
FT DOMAIN 524..730
FT /note="MCM"
FT ZN_FING 380..406
FT /note="C4-type"
FT /evidence="ECO:0000255"
FT REGION 1..81
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 120..220
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 706..709
FT /note="Arginine finger"
FT COMPBIAS 1..35
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 38..79
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 120..157
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 205..220
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 574..581
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000250"
SQ SEQUENCE 961 AA; 108823 MW; B36AB1D62B8D38BB CRC64;
MDDSENNAPS TPGSPGFSTD RLPPNTTTSR GATDPSSYSD DDDDDVVGAE EAEVDPNVLP
EDDGVVAAEE EEDGEDLFND NYLDDYRRMD EQDQYESVGL DDSIEDERNL DEIMADRRAA
EAELDARDVR TGAAPDRKLP RMLHDQDTDE DMSFRRPKRH RANFRPPREP RTPRSDDDGD
GATPSSPGRS QRGMYSGGDV PMTDQTDDDP YEDEFDEEDE MNMYRVQGTL REWVTRDEVR
RFIAKKFKEF LLTYVNPKNE QGEFEYVRLI NEMVLANKCS LEIDYKQFIY IHPNIAIWLA
DAPQSVLEVM EEVAKNVVFD LHKNYRNIHQ KIYVRITNLP VYDQIRNIRQ IHLNTMIRIG
GVVTRRSGVF PQLQQVKFDC SKCGTVLGPF FQNSYTEVKV GSCPECQSKG PFTINVEQTI
YRNYQKLTLQ ESPGIVPAGR LPRYKEVILL NDLIDCARPG EEIEVTGIYT NNFDLSLNTK
NGFPVFATVV EANYVAKKQD LFSAYKLTDE DKAEIEKLAK DPRIGERIVK SIAPSIYGHE
DIKTAIALAM FGGQEKNVKG KHRLRGDINV LLLGDPGTAK SQFLKYVEKT GHRAVYTTGK
GASAVGLTAA VHKDPVTREW TLEGGALVLA DRGICLIDEF DKMNDQDRVS IHEAMEQQSI
SISKAGIVTS LQARCSVIAA ANPIGGRYDS SKTFTQNVEL TDPIISRFDV LCVVKDIVDP
FTDEMLARFV VDSHARSQPK GANLEDRVPT DVEDDPLAAA RQADPDILSQ DMLKKYITYA
KLNVFPKIHD ADLDKISHVY AELRRESSHG QGVPIAVRHI ESIIRMSEAH ARMHLRSYVS
QEDVDMAIRV LLDSFISTQK FGVQKALQKN FRKYMTYKKD YNELLLLLLR TLVKDVLHFE
EIVSGPTTRL THIEVKVEDL KNKAQEYEIY DLRPFFSSAH FRDNNFVLDE GRGIIRHPLA
A
